Targeting Ras-binding domain of ELMO1 by computational nanobody design

Autor: Chunlai Tam, Mutsuko Kukimoto-Niino, Yukako Miyata-Yabuki, Kengo Tsuda, Chiemi Mishima-Tsumagari, Kentaro Ihara, Mio Inoue, Mayumi Yonemochi, Kazuharu Hanada, Takehisa Matsumoto, Mikako Shirouzu, Kam Y. J. Zhang

Publikováno v: Communications Biology, Vol 6, Iss 1, Pp 1-11 (2023)

Structural analysis of the Ras-binding domain (RBD) of ELMO1 alone and in complex with the activator RhoG enables rational engineering and validation of a nanobody targeting ELMO1 RBD.

Externí odkaz: https://doaj.org/article/57ffcbf5665046feb62686a925bac3cb

NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses

Autor: Chunlai Tam, Ashutosh Kumar, Kam Y. J. Zhang

Publikováno v: Pharmaceuticals, Vol 14, Iss 10, p 968 (2021)

Modeling the binding pose of an antibody is a prerequisite to structure-based affinity maturation and design. Without knowing a reliable binding pose, the subsequent structural simulation is largely futile. In this study, we have developed a method o

Externí odkaz: https://doaj.org/article/4f8993e081954a7ab2d3a696596e4636

FPredX: Interpretable models for the prediction of spectral maxima, brightness, and oligomeric states of fluorescent proteins

Autor: Kam Y. J. Zhang, Chunlai Tam

Publikováno v: ProteinsREFERENCES. 90(3)

Fluorescent protein (FP) design is among the challenging protein design problems due to the tradeoffs among multiple properties to be optimized. Despite the accumulated efforts in design and characterization, progress has been slow in gaining a full

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27def2090b019602a5e16d9ddb66356b
https://pubmed.ncbi.nlm.nih.gov/34676905

Coptisine-induced inhibition ofHelicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process

Autor: Jian-Hui Xie, Chaodan Luo, Lixiang Zhu, Chunlai Tam, Hanbin Chen, Kam-Bo Wong, Zi-Ren Su, Qiang Lu, Ping Huang, Lihua Tan, Yifei Xu, Cailan Li

Publikováno v: Journal of Enzyme Inhibition and Medicinal Chemistry
Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 33, Iss 1, Pp 1362-1375 (2018)

In this study, we examined the anti-Helicobactor pylori effects of the main protoberberine-type alkaloids in Rhizoma Coptidis. Coptisine exerted varying antibacterial and bactericidal effects against three standard H. pylori strains and eleven clinic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fc2f4ccde45fafcc1f135ea6161f4d5
https://doi.org/10.1080/14756366.2018.1501044

Targeting Ras-binding domain of ELMO1 by computational nanobody design.

Autor: Tam, Chunlai^1,2 (AUTHOR), Kukimoto-Niino, Mutsuko³ (AUTHOR) kukimoto@riken.jp, Miyata-Yabuki, Yukako⁴ (AUTHOR), Tsuda, Kengo³ (AUTHOR), Mishima-Tsumagari, Chiemi³ (AUTHOR), Ihara, Kentaro³ (AUTHOR), Inoue, Mio³ (AUTHOR), Yonemochi, Mayumi⁴ (AUTHOR), Hanada, Kazuharu³ (AUTHOR), Matsumoto, Takehisa⁴ (AUTHOR), Shirouzu, Mikako^3,4 (AUTHOR), Zhang, Kam Y. J.^1,2 (AUTHOR) kamzhang@riken.jp

Publikováno v: Communications Biology. 3/17/2023, Vol. 6 Issue 1, p1-11. 11p.