Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Chungjung Chou"'
Publikováno v:
PLoS ONE, Vol 9, Iss 9, p e105467 (2014)
Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic
Externí odkaz:
https://doaj.org/article/108a375650b64f6b914fa886f508b214
Autor:
Alexander Deiters, Rajendra Uprety, Ji Luo, Jason W. Chin, Eyal Arbely, Chungjung Chou, J. Zhang
Publikováno v:
Chemical Communications. 52:8529-8532
We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cad1aac6533d59f652361f150f7e175e
https://doi.org/10.1039/c6cc03934k
https://doi.org/10.1039/c6cc03934k
Publikováno v:
Langmuir
Langmuir, American Chemical Society, 2019, 35 (5), pp.1909-1918. ⟨10.1021/acs.langmuir.8b02483⟩
Langmuir, American Chemical Society, 2019, 35 (5), pp.1909-1918. ⟨10.1021/acs.langmuir.8b02483⟩
International audience; Hydrogels are hydrated networks of flexible polymers with versatile biomedical applications, and their resistance to nonspecific protein adsorption is critical. On the other hand, functionalization with other biomacromolecules
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ffb93990453cb982d62eb00901478a8
https://pubmed.ncbi.nlm.nih.gov/30343571
https://pubmed.ncbi.nlm.nih.gov/30343571
Autor:
Robert M. Kelly, Chungjung Chou, Michael W. W. Adams, Jeffrey V. Zurawski, Piyum A. Khatibi, Andrew J. Loder
The archaeon Pyrococcus furiosus is emerging as a metabolic engineering platform for production of fuels and chemicals, such that more must be known about this organism's characteristics in bioprocessing contexts. Its ability to grow at temperatures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41e70ef8e61f4903dce1b9755dbf1dbf
https://europepmc.org/articles/PMC5809124/
https://europepmc.org/articles/PMC5809124/
Publikováno v:
ACS Synthetic Biology
Controlled manipulation of proteins and their function is important in almost all biological disciplines. Here, we demonstrate control of protein activity with light. We present two different applications—light-triggered transcription and light-tri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f3a777baee12dbf5e753b2f7294d97b
https://doi.org/10.1021/sb400192a
https://doi.org/10.1021/sb400192a
Autor:
Kathrin Lang, Alexander Deiters, Chungjung Chou, Jessica Torres-Kolbus, Lloyd S. Davis, Jason W. Chin
Publikováno v:
Nature Chemistry. 4:298-304
The site-specific incorporation of bioorthogonal groups via genetic code expansion provides a powerful general strategy for site-specifically labelling proteins with any probe. However, the slow reactivity of the bioorthogonal functional groups that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ffb00b2259b87c04c6d79cfb7964037
https://doi.org/10.1038/nchem.1250
https://doi.org/10.1038/nchem.1250
Autor:
Alexander Deiters, Chungjung Chou
Publikováno v:
Angewandte Chemie. 123:6971-6974
A general approach for targeted gene modification with precise external control and high spatial and temporal resolution will greatly advance investigations in genetics, gene therapy, and developmental biology. However, traditional methods such as ho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7965785cadaa397352bdfb444a53a3b0
https://doi.org/10.1002/ange.201101157
https://doi.org/10.1002/ange.201101157
Publikováno v:
Chem. Sci.. 2:480-483
Photocrosslinking is an important approach that allows discovery and detailed investigation of protein–protein, protein–oligonucleotide, and protein–small molecule interactions with high temporal and spatial resolution. A major limitation to th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dbc4fa0398527dbddc5141649f1b94e2
https://doi.org/10.1039/c0sc00373e
https://doi.org/10.1039/c0sc00373e
Publikováno v:
ChemBioChem. 11:972-977
A light-activatable bacteriophage T7 RNA polymerase (T7RNAP) has been generated through the site-specific introduction of a photocaged tyrosine residue at the crucial position Tyr639 within the active site of the enzyme. The photocaged tyrosine disru
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9b556d3cd483f6f610d78fd709c172e
https://doi.org/10.1002/cbic.201000041
https://doi.org/10.1002/cbic.201000041
Publikováno v:
Angewandte Chemie. 121:6064-6067
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8b3a5853e8b0232bb8e81de707ecd79
https://doi.org/10.1002/ange.200901115
https://doi.org/10.1002/ange.200901115