Protease S of entomopathogenic bacterium Photorhabdus laumondii: expression, purification and effect on greater wax moth Galleria mellonella.

Autor: Svetlova AO; National Research Centre 'Kurchatov Institute', Moscow, Russia., Karaseva MA; National Research Centre 'Kurchatov Institute', Moscow, Russia., Berdyshev IM; National Research Centre 'Kurchatov Institute', Moscow, Russia., Chukhontseva KN; National Research Centre 'Kurchatov Institute', Moscow, Russia., Pobeguts OV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Galyamina MA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Smirnov IP; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Polyakov NB; Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia., Zavialova MG; Skolkovo Institute of Science and Technology, Moscow, Russia.; Institute of Biomedical Chemistry, Moscow, Russia., Kostrov SV; National Research Centre 'Kurchatov Institute', Moscow, Russia., Demidyuk IV; National Research Centre 'Kurchatov Institute', Moscow, Russia. ilyaduk@yandex.ru.

Publikováno v: Molecular biology reports [Mol Biol Rep] 2024 Jun 01; Vol. 51 (1), pp. 713. Date of Electronic Publication: 2024 Jun 01.

NMR structure of emfourin, a novel protein metalloprotease inhibitor: Insights into the mechanism of action.

Autor: Bozin TN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia; National Research Centre 'Kurchatov Institute', Moscow, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Berdyshev IM; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Chukhontseva KN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Karaseva MA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Konarev PV; Shubnikov Institute of Crystallography of the Federal Scientific Research Centre 'Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russia., Varizhuk AM; Moscow Institute of Physics and Technology, State University, Dolgoprudny, Russia., Lesovoy DM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Arseniev AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Kostrov SV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Bocharov EV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Moscow Institute of Physics and Technology, State University, Dolgoprudny, Russia., Demidyuk IV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia. Electronic address: duk@img.ras.ru.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2023 Apr; Vol. 299 (4), pp. 104585. Date of Electronic Publication: 2023 Mar 06.

The protealysin operon encodes emfourin, a prototype of a novel family of protein metalloprotease inhibitors.

Autor: Chukhontseva KN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Berdyshev IM; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Safina DR; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Karaseva MA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Bozin TN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia; National Research Centre 'Kurchatov Institute', Moscow, Russia., Salnikov VV; Kazan Federal University, Kazan, Russia; Kazan Institute of Biochemistry and Biophysics, Federal Research Center 'Kazan Scientific Center of the Russian Academy of Sciences', Kazan, Russia., Konarev PV; Shubnikov Institute of Crystallography of the Federal Scientific Research Centre 'Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russia., Volkov VV; Shubnikov Institute of Crystallography of the Federal Scientific Research Centre 'Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russia., Grishin AV; Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia., Kozlovskiy VI; Chernogolovka Branch of the Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia., Kostrov SV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Demidyuk IV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia. Electronic address: duk@img.ras.ru.

Publikováno v: International journal of biological macromolecules [Int J Biol Macromol] 2021 Feb 01; Vol. 169, pp. 583-596. Date of Electronic Publication: 2020 Dec 29.

Protealysin is not Secreted Constitutively.

Autor: Chukhontseva KN; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation., Salnikov VV; Kazan Federal University, Kazan, Russian Federation.; Kazan Institute of Biochemistry and Biophysics, Federal Research Center 'Kazan Scientific Center of Russian Academy of Sciences', Kazan, Russian Federation., Morenkov OS; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russian Federation., Kostrov SV; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation., Demidyuk IV; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation.

Publikováno v: Protein and peptide letters [Protein Pept Lett] 2019; Vol. 26 (3), pp. 221-226.