Zobrazeno 1 - 10
of 134
pro vyhledávání: '"Chuichi Hirayama"'
Autor:
Masayo Sakata, Kazuhiro Yanagi, Masashi Kunitake, Chuichi Hirayama, Minoru Nakayama, Mitsuru Sasaki
Publikováno v:
Journal of Liquid Chromatography & Related Technologies. 29:2499-2512
This paper describes a method for the selective removal of DNA from various cellular products using columns packed with polycation‐immobilized cellulose beads. Polyethyleneimine (PEI), poly‐N,N‐dimethylaminopropylacrylamide (poly(DAPA)) and pol
Publikováno v:
Chromatographia. 62:465-470
This paper describes a method for selective removal of DNA from various cellular products using columns packed with cross-linked poly(ethyleneimine) (PEI) beads or cross-linked N,N-dimethylaminopropylacrylamide (DMAPAA) beads. Each bead type showed a
Publikováno v:
KOBUNSHI RONBUNSHU. 62:29-35
プルランから得られた橋かけプルラン球状粒子を基体として, 各種プルラン球状イオン交換体を調製した. これら基体に2-クロロエチルジエチルアミン塩酸塩を反応させたジエチルアミノ
Autor:
Hiroshi Mizokami, Chuichi Hirayama, Minoru Nakayama, Masashi Kunitake, Masayo Sakata, Takashi Kamada
Publikováno v:
Journal of Chromatography A. 1030:117-122
To remove nucleic acids from cellular products as drugs, cross-linked N,N-dimethylaminopropylacrylamide (DMP) particles with cationic functional groups were prepared. The particle's hydrophobicity and its anion-exchange capacity were easily adjusted
Autor:
Hiroshi Mizokami, Chuichi Hirayama, Kenji Soejima, Tatsufumi Onchi, Keishin Sugawara, Shinichi Furukawa, Satoshi Koyanagi, Toshihiro Maeda, Hideo Yoshizaki
Publikováno v:
Journal of Bioscience and Bioengineering. 97:95-98
This study presents a novel method for the production and purification of annexin V using cation exchange chromatography in the presence of calcium ions at neutral or alkaline pH. This method enables the handling of a large quantity of sample at one
Autor:
Chuichi Hirayama, Toshihiko Sakurai, Dai Kato, Masahiko Takeuchi, Masayo Sakata, Shin Ichi Furukawa, Hiroshi Mizokami, Masashi Kunitake
Publikováno v:
Biomaterials. 24:4253-4264
A variety of neutral and cationic polymers based on polyamino acids were prepared and investigated as microcarriers for cell attachment and growth. Among neutral polymer particles including the alkylated poly(gamma-methyl L-glutamate) (PG) particles,
Autor:
Kayo Yoshida, Yukio Asami, Aya Katada, Masayo Sakata, Kentarou Yamanaka, Chuichi Hirayama, Tomomi Harada, Masashi Kunitake, Kiyoshi Sakai, Tan Chun Teng
Publikováno v:
Chemistry of Materials. 15:4091-4097
Dendritic polymer-modified porous silica particles prepared by a poly(amidoamine) (PAMAM) dendrimer synthesis reaction were characterized by elementary analysis (EA) and size exclusion chromatography (SEC). EA demonstrated the successful alternate gr
Publikováno v:
Journal of the American Chemical Society. 125:5057-5065
"Nanotube" structures of the alpha-, beta-, and gamma-cyclodextrins (CyD's), which are similar to that of CyD-polyrotaxane, were constructed by potential-controlled adsorption onto Au(111) surfaces in sodium perchlorate solution without a threaded po
Autor:
Hiroshi Mizokami, Masashi Kunitake, Kouchi Matsumoto, Nagi Obaru, Chuichi Hirayama, Masayo Sakata
Publikováno v:
Journal of Liquid Chromatography & Related Technologies. 26:231-246
To remove nucleic acids from a solution of a cell product used as a drug, water‐insoluble poly(ethyleneimine) (PEI) spherical particles were prepared by suspension cross‐linking with PEI and chloromethyloxirane. The PEI content of the particles w
Autor:
Masayo Sakata, Chuichi Hirayama
Publikováno v:
Journal of Chromatography B. 781:419-432
Endotoxins, constituents of cell walls of gram-negative bacteria, are potential contaminants of the protein solutions originating from biological products. Such contaminants have to be removed from solutions used for intravenous administration, becau