Combining recombinase polymerase amplification with tyrosine modified 2'-deoxyuridine-5'-triphosphate for direct voltammetric detection of double-stranded DNA: Application to potato pathogen Dickeya solani.

Autor: Suprun EV; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow, 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia. Electronic address: lenasuprun@mail.ru., Khmeleva SA; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Duskaev IF; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow, 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Ptitsyn KG; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Kurbatov LK; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street, 32, Moscow, 119991, Russia., Radko SP; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow, 119121, Russia.

Publikováno v: Talanta [Talanta] 2024 Jun 01; Vol. 273, pp. 125841. Date of Electronic Publication: 2024 Feb 29.

Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Decrease PCR Efficiency.

Autor: Zasedateleva OA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Surzhikov SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Barsky VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia.

Publikováno v: International journal of molecular sciences [Int J Mol Sci] 2023 Sep 04; Vol. 24 (17). Date of Electronic Publication: 2023 Sep 04.

Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labelling.

Autor: Kuznetsova VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: kuzneimb@gmail.com., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Guseinov TO; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Miftakhov RA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Solyev PN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Novikov RA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Levashova AI; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: chud@eimb.ru.

Publikováno v: Bioorganic chemistry [Bioorg Chem] 2023 Feb; Vol. 131, pp. 106315. Date of Electronic Publication: 2022 Dec 10.

[New Method for Evaluating the Substrate Efficiency of Modified Deoxynucleoside Triphosphates for Selex].

Autor: Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.; lapa@biochip.ru., Antipova OS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

Publikováno v: Molekuliarnaia biologiia [Mol Biol (Mosk)] 2022 May-Jun; Vol. 56 (3), pp. 521-527.

[Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens].

Autor: Lapa SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.; lapa@biochip.ru., Miftakhov RA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Klochikhina ES; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Ammur YI; Mechnikov Institute of Vaccines and Serums, Moscow, 105064 Russia., Blagodatskikh SA; Scientific Center of Applied Microbiology and Biotechnology, Obolensk, 142279 Russia., Shershov VE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Chudinov AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

Publikováno v: Molekuliarnaia biologiia [Mol Biol (Mosk)] 2021 Nov-Dec; Vol. 55 (6), pp. 944-955.