Zobrazeno 1 - 10
of 2 019
pro vyhledávání: '"Chromatofocusing"'
Experimental protocol for pH-gradient chromatofocusing of the native and desialylated human apo-transferrin. Supplemental information for the published paper: Friganovi��, Tomislav; Toma��i��, Antonela; ��eba, Tino; Biru��, Ivan;
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::226540393360832c8e66b14edc2a6d86
https://www.bib.irb.hr/1112990
https://www.bib.irb.hr/1112990
Publikováno v:
Electrophoresis, 39(8)
Electrophoresis, 39(8), 1031-1039. Wiley-VCH Verlag
Electrophoresis
Electrophoresis, 39(8), 1031-1039. Wiley-VCH Verlag
Electrophoresis
A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro‐column, pH gradient generation, and fraction collection in a single device. Using a sieve micro‐valve, anion exchange particles were packed in
Autor:
Jayne Murphy, Gary Walsh
Publikováno v:
Extremophiles. 23:783-792
Intracellular β-galactosidase (E.C 3.2.1.23) produced by the thermoacidophilic archeon Picrophilus torridus DSM 9790 was purified to homogeneity using a combination of DEAE Sepharose, gel filtration, hydroxyapatite and chromatofocusing chromatograph
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1984 Jan 01. 81(1), 238-242.
Externí odkaz:
https://www.jstor.org/stable/23197
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1983 Apr . 80(8), 2216-2220.
Externí odkaz:
https://www.jstor.org/stable/13481
Autor:
Raphael Wolfisberg, Thomas Nowak, Andreas Hemmerle, Carlos Ros, Nathan J. Roth, Oliver Caliaro, Remo Leisi
Anion-exchange chromatography (AEX) is used in the downstream purification of monoclonal antibodies to remove impurities and potential viral contamination based on electrostatic interactions. Although the isoelectric point (pI) of viruses is consider
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1ca06b6d654ec8380a9a77cea80aaf3
Autor:
Deldari, Sevda
For personalized medicines, national emergencies, military operations, and related applications there is a growing need to produce single-dose levels of therapeutic proteins at the point of care. To satisfy this need, the ?biologically-derived medici
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::047470b79a297f105a19e48e7eb64475
Autor:
Liu, Yang
Chromatofocusing is a special form of ion-exchange chromatography (IEX) that uses a self-generated pH gradient that travels through the column as a set of retained waves. The nonlinear adsorption behavior and displacement effect under mass overloaded
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6ff2d8fc1934ddac8e060589047e33c8
Publikováno v:
OENO One, Vol 24, Iss 1, Pp 13-28 (1990)
Les auteurs étudient les glycoprotéines dans des moûts de raisins blancs. Ces glycoprotéines sont fractionnées par chromatofocalisation et caractérisées par différentes techniques séparatives : CLHP, électrophorèse, SDS-PAGE, analyse des a
Externí odkaz:
https://doaj.org/article/9526d07ebf90465ca224ff3db4532664
Publikováno v:
Applied Biochemistry and Biotechnology. 183:318-331
Viral clearance is a critical aspect of biopharmaceutical manufacturing process validation. To determine the viral clearance efficacy of downstream chromatography and filtration steps, live viral "spiking" studies are conducted with model mammalian v