Zobrazeno 1 - 10
of 573
pro vyhledávání: '"Chromatin Immunoprecipitation (ChIP)"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103163- (2024)
Summary: Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here
Externí odkaz:
https://doaj.org/article/045462ed796443e6be44479291f352c1
Autor:
Andrew Katznelson, Kenneth Zaret
Publikováno v:
STAR Protocols, Vol 5, Iss 1, Pp 102866- (2024)
Summary: Cleavage under targets & release using nuclease (CUT&RUN) is a technique for identifying genomic sites where proteins or histone modifications are present in chromatin in permeabilized cells. Here, we present a fluorescence-based protocol to
Externí odkaz:
https://doaj.org/article/1a617f4304af490c9177fa5a878ea822
Publikováno v:
Data in Brief, Vol 50, Iss , Pp 109499- (2023)
The tumor suppressor p53 exerts its role mainly as a transcription factor. The TP53 gene, which encodes the p53 protein, is the most commonly mutated gene in human cancers, particularly triple negative breast cancer (TNBC). Variations in the TP53 gen
Externí odkaz:
https://doaj.org/article/99323abafef0442bb7123cb86d6ffda9
Autor:
Deshui Yu, Renchun Fan, Ling Zhang, Pengya Xue, Libing Liao, Meizhen Hu, Yanjun Cheng, Jine Li, Ting Qi, Shaojuan Jing, Qiuyun Wang, Arvind Bhatt, Qian-Hua Shen
Publikováno v:
Crop Journal, Vol 11, Iss 1, Pp 99-107 (2023)
Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner. In barley, HvWRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus, Blumeria graminis
Externí odkaz:
https://doaj.org/article/aae86eea55e64a4d9cd0ea1bc0a045f5
Akademický článek
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Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102109- (2023)
Summary: Transduction with lentiviral vectors is a useful approach to study the molecular function of specific genes in mammalian cells. Here, we present a calcium phosphate-based transfection protocol that guarantees highly efficient production and
Externí odkaz:
https://doaj.org/article/5d8420be8e8f45c8b9da30491da3e358
Autor:
Yuta Hiraike
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102093- (2023)
Summary: Chromatin immunoprecipitation (ChIP) experiments with differentiated adipocytes are challenging because lipid droplets interfere with immunoprecipitation efficiency. Here, the author describes optimized procedures to minimize the burden of l
Externí odkaz:
https://doaj.org/article/612fb670630d478884d1076f9fb8bba9
Autor:
Ajit K. Sharma, Amira Mohammed Fitieh, Andrew J. Locke, Jana Yasser Hafez Ali, Ismail Hassan Ismail
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 101917- (2023)
Summary: Here, we present a chromatin-immunoprecipitation-based protocol to quantify the recruitment of proteins adjacent to site-specific DNA double-strand breaks (DSBs), such as proteins involved in DSB repair. We describe steps to induce DSBs in U
Externí odkaz:
https://doaj.org/article/b7ab18c768fd4966b7d86e69e1696ec8
Publikováno v:
STAR Protocols, Vol 3, Iss 4, Pp 101918- (2022)
Summary: Genome-wide mapping of transcription factors (TFs) is critical to understand their functions. In chromatin immunoprecipitation (ChIP)-seq assay, it’s challenging to study recruitment of low-abundant TFs transiently boud to the genome. Here
Externí odkaz:
https://doaj.org/article/42c260d40ab4412a98ae11e206d6fa94
Akademický článek
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