Zobrazeno 1 - 10
of 48
pro vyhledávání: '"Christopher S. Cohan"'
Publikováno v:
Methods in cell biology. 145
This chapter covers methods that are useful for the in vitro culture and live-cell study of insect spermatocytes in general and of crane-fly spermatocytes in particular. The merits of crane-fly spermatocytes are detailed in the Introduction section.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b53fdfc5ba659c7deac6ce9b3cfb21dd
https://pubmed.ncbi.nlm.nih.gov/29957207
https://pubmed.ncbi.nlm.nih.gov/29957207
Autor:
Hui Yin Tan, Cong Yu, Meerim Choi, Christopher S. Cohan, Adam J. Stanenas, Piero R. Bianco, Alicia K. Byrd, Kevin D. Raney
Publikováno v:
Genes to Cells. 21:163-184
The E. coli single-stranded DNA-binding protein (SSB) binds to the fork DNA helicases RecG and PriA in vitro. Typically for binding to occur, 1.3 M ammonium sulfate must be present, bringing into question the validity of these data as these are non-p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::008aab695060490574bacbeea1ecc305
https://doi.org/10.1111/gtc.12334
https://doi.org/10.1111/gtc.12334
This chapter covers methods that are useful for the in vitro culture and live-cell study of insect spermatocytes in general and of crane-fly spermatocytes in particular. The merits of crane-fly spermatocytes are detailed in the Introduction section.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::946ce19ce1f645b265f90f694f7c2ce9
https://doi.org/10.1016/bs.mcb.2018.03.027
https://doi.org/10.1016/bs.mcb.2018.03.027
Publikováno v:
Molecular Biology of the Cell
Experiments reveal pac-man motility in kinetochores of X-Y chromosomes, even though their normal behavior is dominated by traction fiber mechanics. A laser microbeam is used to release kinetochores in anaphase from tension. There is a poleward motion
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7af82072366031d63a6781ad61a7ada8
http://europepmc.org/articles/PMC3418308
http://europepmc.org/articles/PMC3418308
Autor:
Christopher S. Cohan, Adam G. Stanenas, Piero R. Bianco, Kevin D. Raney, Meerim Choi, Juan Liu, Alicia K. Byrd
Publikováno v:
Protein Science. 20:1005-1020
The Escherichia coli single-stranded DNA binding protein (SSB) is a central player in DNA metabolism where it organizes genome maintenance complexes and stabilizes single-stranded DNA (ssDNA) intermediates generated during DNA processing. Due to the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::846a0acf464f2e333e9f12bb7c988975
https://doi.org/10.1002/pro.633
https://doi.org/10.1002/pro.633
Publikováno v:
Molecular Biology of the Cell. 15:5724-5732
Microtubule flux in spindles of insect spermatocytes, long-used models for studies on chromosome behavior during meiosis, was revealed after iontophoretic microinjection of rhodamine-conjugated (rh)-tubulin and fluorescent speckle microscopy. In time
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50bd309f701e26f21a9f69425b17c61d
https://doi.org/10.1091/mbc.e04-08-0750
https://doi.org/10.1091/mbc.e04-08-0750
Publikováno v:
The Journal of Cell Biology
Ît is commonly believed that growth cone turning during pathfinding is initiated by reorganization of actin filaments in response to guidance cues, which then affects microtubule structure to complete the turning process. However, a major unanswere
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dd2497db93c79251ea23df6d8fc352e
http://europepmc.org/articles/PMC2173410
http://europepmc.org/articles/PMC2173410
Publikováno v:
Cell Motility and the Cytoskeleton. 48:109-120
Growth cones at the distal tips of growing nerve axons contain bundles of actin filaments distributed throughout the lamellipodium and that project into filopodia. The regulation of actin bundling by specific actin binding proteins is likely to play
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4f3f6228786864459ff035c41ac4153
https://doi.org/10.1002/1097-0169(200102)48:2<109::aid-cm1002>3.0.co
https://doi.org/10.1002/1097-0169(200102)48:2<109::aid-cm1002>3.0.co
Publikováno v:
The Journal of Neuroscience. 19:7971-7982
The ability of calcium (Ca(2+)) to effect changes in growth cone motility requires remodeling of the actin cytoskeleton. To understand the mechanisms involved, we evaluated the effect of elevated intracellular calcium ([Ca(2+)](i)) on actin bundle dy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3529c4618fc589231fd270841cf2a60b
https://doi.org/10.1523/jneurosci.19-18-07971.1999
https://doi.org/10.1523/jneurosci.19-18-07971.1999
Publikováno v:
Journal of Membrane Biology. 171:151-169
Neuronal shape and volume changes require accompanying cell surface adjustments. In response to osmotic perturbations, neurons show evidence of surface area regulation; shrinking neurons invaginate membrane at the substratum, pinch off vacuoles, and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ecd6d87672d03d6e973674101a6ce8e
https://doi.org/10.1007/s002329900567
https://doi.org/10.1007/s002329900567