Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Christopher S, Frei"'
Publikováno v:
BioTechniques, Vol 59, Iss 3, Pp 157-161 (2015)
Iterative screening of expressed protein libraries using fluorescence-activated cell sorting (FACS) typically involves culturing the pooled clones after each sort. In these experiments, if cell viability is compromised by the sort conditions and/or e
Externí odkaz:
https://doaj.org/article/cbb0d637176f41c6b0c5464c8651fc4f
Publikováno v:
Protein Engineering, Design and Selection. 31:213-220
Customized transcription factors that control gene expression in response to small molecules can act as endogenous molecular biosensors and are valuable tools for synthetic biology. We previously engineered the Escherichia coli regulatory protein Ara
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5bc55a690df95fea102702317184336
https://doi.org/10.1093/protein/gzy024
https://doi.org/10.1093/protein/gzy024
Autor:
Shuai Qian, Markus Sutter, Patrick C. Cirino, Christopher S Frei, Samuel Deutsch, Zhiqing Wang
Publikováno v:
Protein Science. 25:804-814
The Escherichia coli regulatory protein AraC regulates expression of ara genes in response to l-arabinose. In efforts to develop genetically encoded molecular reporters, we previously engineered an AraC variant that responds to the compound triacetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a666f5931626a49ad3a359e643f3b6fc
https://doi.org/10.1002/pro.2873
https://doi.org/10.1002/pro.2873
Autor:
Christopher S, Frei, Zhiqing, Wang, Shuai, Qian, Samuel, Deutsch, Markus, Sutter, Patrick C, Cirino
Publikováno v:
Protein science : a publication of the Protein Society. 25(4)
The Escherichia coli regulatory protein AraC regulates expression of ara genes in response to l‐arabinose. In efforts to develop genetically encoded molecular reporters, we previously engineered an AraC variant that responds to the compound triacet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2a0781df2d53312dc415f522119deea7
https://pubmed.ncbi.nlm.nih.gov/26749125
https://pubmed.ncbi.nlm.nih.gov/26749125
Publikováno v:
BioTechniques. 59(3)
Iterative screening of expressed protein libraries using fluorescence-activated cell sorting (FACS) typically involves culturing the pooled clones after each sort. In these experiments, if cell viability is compromised by the sort conditions and/or e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::027d0cee53a6f49ab5bdb246b3821759
https://pubmed.ncbi.nlm.nih.gov/26345509
https://pubmed.ncbi.nlm.nih.gov/26345509
Publikováno v:
Biotechnology journal. 7(4)
Nature takes advantage of the malleability of protein and RNA sequence and structure to employ these macromolecules as molecular reporters whose conformation and functional roles depend on the presence of a specific ligand (an "effector" molecule). B
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::520ef879de1de0b251b1da2c38efd302
https://pubmed.ncbi.nlm.nih.gov/22031507
https://pubmed.ncbi.nlm.nih.gov/22031507
Autor:
Christopher S Frei, Patrick C. Cirino
Publikováno v:
Protein Engineering and Design
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b4a57b9f3a471ddfcd4478f93af77f31
https://doi.org/10.1201/9781420076592.ch6
https://doi.org/10.1201/9781420076592.ch6
Publikováno v:
Biotechnology letters. 30(9)
Escherichia coli W3110 was previously engineered to produce xylitol from a mixture of glucose plus xylose by expressing xylose reductase (CbXR) and deleting xylulokinase (DeltaxylB), combined with either plasmid-based expression of a xylose transport
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dfcd34d64e980425c50df4d655c1a98
https://pubmed.ncbi.nlm.nih.gov/18414795
https://pubmed.ncbi.nlm.nih.gov/18414795