Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1

Autor: Kim M. Summers, Hiu-Gwen Tsang, Brendan Corcoran, Greg R. Markby, Simon G. Lillico, Violeta Trejo-Reveles, Mary E. B. McCulloch, C. Bruce A. Whitelaw, Vicky E MacRae, Christopher Proudfoot

This paper describes a genome editing project using CRISPR-Cas9. The objective was to create a large animal model of human Marfan syndrome by targeting the FBN1 gene of the pig, Sus scrofa, using a single guide and non-homologous end joining which wa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::15525d670117e564190d8238622b0c34
https://doi.org/10.1101/2020.07.20.213108

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

Autor: Stephen McCleary, C. Bruce A. Whitelaw, Jane C. Edwards, Maeve Ballantyne, Simon G. Lillico, Samantha Watson, Christine Tait-Burkard, Rebecca Strong, Christopher Proudfoot, Helen Crooke, Ronan R. McCarthy, Emma L. Howes, Pedro J. Sánchez-Cordón, Alan J. Mileham, Lisa M. Stevens, Falko Steinbach, Alejandro Núñez

Publikováno v: McCleary, S, Strong, R, McCarthy, R R, Edwards, J C, Howes, E L, Stevens, L M, Sanchez-Cordon, P J, Nunez, A, Watson, S, Mileham, A J, Lillico, S, Tait-Burkard, C, Proudfoot, C, Ballantyne, M, Whitelaw, B, Steinbach, F & Crooke, H R 2020, ' Substitution of warthog NF-B motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus ', Scientific Reports . https://doi.org/10.1038/s41598-020-65808-1
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports

Copyright © 2020 The Author(s). African swine fever virus (ASFV) causes a lethal, haemorrhagic disease in domestic swine that threatens pig production across the globe. Unlike domestic pigs, warthogs, which are wildlife hosts of the virus, do not su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::987412e3583baafe9634dfcb5b1f2e52
http://bura.brunel.ac.uk/handle/2438/20960

Livestock breeding for the 21st century: the promise of the editing revolution

Autor: Gus R. McFarlane, Christopher Proudfoot, Simon G. Lillico, Bruce Whitelaw

Publikováno v: Frontiers of Agricultural Science and Engineering, Vol 7, Iss 2, Pp 129-135 (2020)

In recent years there has been a veritable explosion in the use of genome editors to create site-specific changes, both in vitro and in vivo, to the genomes of a multitude of species for both basic research and biotechnology. Livestock, which form a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ecb3e5f6c26cae3143af5455ae6a15c
https://doi.org/10.15302/j-fase-2019304

Highly efficient targeted chromosome deletions using CRISPR/Cas9

Autor: C. Bruce A. Whitelaw, Simon G. Lillico, Alan J. Mileham, Zuyong He, David G. McLaren, Christopher Proudfoot

Publikováno v: Biotechnology and Bioengineering. 112:1060-1064

The CRISPR/Cas9 system has emerged as an intriguing new technology for genome engineering. It utilizes the bacterial endonuclease Cas9 which, when delivered to eukaryotic cells in conjunction with a user-specified small guide RNA (gRNA), cleaves the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::23a26791de31668923cbe1b140fc6bb4
https://doi.org/10.1002/bit.25490

Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells

Autor: Zuyong He, Simon G. Lillico, Christopher Proudfoot, C. Bruce A. Whitelaw

Publikováno v: He, Z, Proudfoot, C, Whitelaw, C & Lillico, S 2016, ' Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells ', SpringerPlus, vol. 5, no. 1, 814 . https://doi.org/10.1186/s40064-016-2536-3
SpringerPlus

Background Genome editors such as CRISPR/Cas9 and TALENs are at the forefront of research into methodologies for targeted modification of the mammalian genome. To date few comparative studies have been carried out to investigate the difference of gen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb2fb4ffb9050a20300b2805945240c8
https://www.pure.ed.ac.uk/ws/files/26394346/art_3A10.1186_2Fs40064_016_2536_3.pdf