Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Christopher M. Whitford"'
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102190- (2023)
Summary: Polycyclopropanated (POP) compounds show promise as fuels as their energy density can be greater than jet and rocket fuels in current use, but realizing their full potential requires significant development. This protocol guides the producti
Externí odkaz:
https://doaj.org/article/1cd7f3ddb9584d64b4110ea1135dab88
Autor:
Tetiana Gren, Christopher M. Whitford, Omkar S. Mohite, Tue S. Jørgensen, Eftychia E. Kontou, Julie B. Nielsen, Sang Yup Lee, Tilmann Weber
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Abstract Streptomyces griseofuscus DSM 40191 is a fast growing Streptomyces strain that remains largely underexplored as a heterologous host. Here, we report the genome mining of S. griseofuscus, followed by the detailed exploration of its phenotype,
Externí odkaz:
https://doaj.org/article/1d6bc7c1088b4caf88db11a4fa49fa8b
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
CRISPR prime editing enables double-strand break free engineering of the genome. Here the authors present a toolkit for prime editing in E. coli.
Externí odkaz:
https://doaj.org/article/6836131df35f472ab3558ca5e1c7dd02
Autor:
Christopher M. Whitford, Saskia Dymek, Denise Kerkhoff, Camilla März, Olga Schmidt, Maximilian Edich, Julian Droste, Boas Pucker, Christian Rückert, Jörn Kalinowski
Publikováno v:
Journal of Biological Engineering, Vol 12, Iss 1, Pp 1-28 (2018)
Abstract Background Biosafety is a key aspect in the international Genetically Engineered Machine (iGEM) competition, which offers student teams an amazing opportunity to pursue their own research projects in the field of Synthetic Biology. iGEM proj
Externí odkaz:
https://doaj.org/article/ca2dc8ae866b43efb79edcbbc0b9ae6f
Autor:
Christopher M. Whitford, Peter Gockel, David Faurdal, Tetiana Gren, Renata Sigrist, Tilmann Weber
Type I CRISPR systems are widespread in bacteria and archaea. The main differences compared to more widely applied type II systems are multi-effector CASCADE needed for crRNA processing and target recognition, as well as the processive nature of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07318b628f574799016657597675a81d
https://doi.org/10.1101/2023.05.09.539971
https://doi.org/10.1101/2023.05.09.539971
Autor:
Yaojun Tong, Tue Sparholt Jørgensen, Tilmann Weber, Sang Yup Lee, Christopher M. Whitford, Kai Blin
Publikováno v:
Nature Protocols. 15:2470-2502
Streptomycetes are prominent sources of bioactive natural products, but metabolic engineering of the natural products of these organisms is greatly hindered by relatively inefficient genetic manipulation approaches. New advances in genome editing tec
Publikováno v:
Essays in biochemistry. 65(2)
Streptomycetes are producers of a wide range of specialized metabolites of great medicinal and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the drivers of the golden age of antibiotics in the 1950s and 1960s, te
Publikováno v:
Microbiology Resource Announcements
Gren, T, Jørgensen, T S, Whitford, C M & Weber, T 2020, ' High-Quality Sequencing, Assembly, and Annotation of the Streptomyces griseofuscus DSM 40191 Genome ', Microbiology Resource Announcements, vol. 9, no. 47, pp. e01100-20-e01100-20 . https://doi.org/10.1128/MRA.01100-20
Gren, T, Jørgensen, T S, Whitford, C M & Weber, T 2020, ' High-Quality Sequencing, Assembly, and Annotation of the Streptomyces griseofuscus DSM 40191 Genome ', Microbiology Resource Announcements, vol. 9, no. 47, pp. e01100-20-e01100-20 . https://doi.org/10.1128/MRA.01100-20
Here, we report the sequencing, assembly, and annotation of the genome of Streptomyces griseofuscus DSM 40191. The genome of S. griseofuscus was sequenced using PacBio and Illumina technologies. It consists of a linear 8,721,740-bp chromosome and thr
Autor:
Tue Sparholt Jørgensen, Tetiana Gren, Christopher M. Whitford, Julie B. Nielsen, Tilmann Weber, Eftychia E. Kontou, Sang Yup Lee, Omkar Satyavan Mohite
Streptomyces griseofuscusDSM 40191 is a fast growingStreptomycesstrain that remains largely underexplored as a heterologous host. Here, we report the genome mining ofS. griseofuscus, followed by the detailed exploration of its phenotype, including pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b5b1ca87ca040d2dea95f2e889af6d6c
https://doi.org/10.1101/2020.11.06.372458
https://doi.org/10.1101/2020.11.06.372458
In most prokaryotes, missing and poorly active non-homologous end joining (NHEJ) DNA repair pathways heavily restrict the direct application of CRISPR-Cas for DNA double-strand break (DSB)-based genome engineering without providing editing templates.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::063edf8892c126fa6b29ff4e7b823b78
https://doi.org/10.1101/2020.09.02.279141
https://doi.org/10.1101/2020.09.02.279141