Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Christopher M. Topham"'
Autor:
Thomas Walther, Christopher M. Topham, Romain Irague, Clément Auriol, Audrey Baylac, Hélène Cordier, Clémentine Dressaire, Luce Lozano-Huguet, Nathalie Tarrat, Nelly Martineau, Marion Stodel, Yannick Malbert, Marc Maestracci, Robert Huet, Isabelle André, Magali Remaud-Siméon, Jean Marie François
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
2,4-Dihydroxybutyric acid has potential to be a precursor to a range of industrially important products, however a natural metabolic pathway for its synthesis does not exist. Here the authors rationally design a synthetic pathway inE. coliby engineer
Externí odkaz:
https://doaj.org/article/40ffedfba15045e1b9289f7018cf9337
Autor:
Romain Irague, Christopher M Topham, Nelly Martineau, Audrey Baylac, Clément Auriol, Thomas Walther, Jean-Marie François, Isabelle André, Magali Remaud-Siméon
Publikováno v:
PLoS ONE, Vol 13, Iss 2, p e0193036 (2018)
An end-point ADP/NAD+ acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD+ co-enzyme formation by the conventio
Externí odkaz:
https://doaj.org/article/9a630c2d6ddf461a9b721db2b7d8872e
Autor:
B. David, Michel Chateau, Christopher M. Topham, M. Cot, E. Guémard, Vincent Tournier, E. Kamionka, A. Gilles, Gianluca Cioci, M. Dalibey, Elisabeth Moya-Leclair, C. Folgoas, Sophie Barbe, Alain Marty, Marie-Laure Desrousseaux, S. Gavalda, J. Nomme, Sophie Duquesne, Isabelle André, Helene Texier
Publikováno v:
Nature
Nature, Nature Publishing Group, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩
Nature, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩
Nature, Nature Publishing Group, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩
Nature, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩
Present estimates suggest that of the 359 million tons of plastics produced annually worldwide1, 150–200 million tons accumulate in landfill or in the natural environment2. Poly(ethylene terephthalate) (PET) is the most abundant polyester plastic,
Autor:
Christopher M. Topham, Jeremy C. Smith
Publikováno v:
Journal of computer-aided molecular design. 35(3)
Sequence-specific targeting of double-stranded DNA and non-coding RNA via triple-helix-forming peptide nucleic acids (PNAs) has attracted considerable attention in therapeutic, diagnostic and nanotechnological fields. An E-base (3-oxo-2,3-dihydropyri
Publikováno v:
Journal of Chemical Theory and Computation
Journal of Chemical Theory and Computation, American Chemical Society, 2016, 12 (8), pp.4146-4168. ⟨10.1021/acs.jctc.6b00090⟩
Journal of Chemical Theory and Computation, 2016, 12 (8), pp.4146-4168. ⟨10.1021/acs.jctc.6b00090⟩
Journal of Chemical Theory and Computation, American Chemical Society, 2016, 12 (8), pp.4146-4168. ⟨10.1021/acs.jctc.6b00090⟩
Journal of Chemical Theory and Computation, 2016, 12 (8), pp.4146-4168. ⟨10.1021/acs.jctc.6b00090⟩
Shortcomings in the definition of effective free-energy surfaces of proteins are recognized to be a major contributory factor responsible for the low success rates of existing automated methods for computational protein design (CPD). The formulation
Autor:
Cláudio Remedios Frazão, Yoann Malbert, Christopher M. Topham, Jean Marie François, Thomas Walther
Publikováno v:
Biochemical Journal
Biochemical Journal, Portland Press, 2018, 475 (23), pp.3887-3901. ⟨10.1042/BCJ20180765⟩
Biochemical Journal, 2018, 475 (23), pp.3887-3901. ⟨10.1042/BCJ20180765⟩
Biochemical Journal, Portland Press, 2018, 475 (23), pp.3887-3901. ⟨10.1042/BCJ20180765⟩
Biochemical Journal, 2018, 475 (23), pp.3887-3901. ⟨10.1042/BCJ20180765⟩
A synthetic pathway for the production of 2,4-dihydroxybutyric acid from homoserine, composed of two consecutive enzymatic reaction steps has been recently reported. An important step in this pathway consists in the reduction of 2-keto-4-hydroxybutyr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d0bd00abd957dae8df1fbe14ced31a5
https://hal.archives-ouvertes.fr/hal-01978892
https://hal.archives-ouvertes.fr/hal-01978892
Autor:
Isabelle André, Nelly Martineau, Audrey Baylac, Clément Auriol, Magali Remaud-Simeon, Jean-Marie François, Christopher M. Topham, Romain Irague, Thomas Walther
Publikováno v:
PLoS ONE
PLoS ONE, Public Library of Science, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, Vol 13, Iss 2, p e0193036 (2018)
Plos One 2 (13), 15 p.. (2018)
PLoS ONE, Public Library of Science, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, Vol 13, Iss 2, p e0193036 (2018)
Plos One 2 (13), 15 p.. (2018)
An end-point ADP/NAD(+) acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD(+) co-enzyme formation by the conve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29381b9349a19b8604e314ec1ddd02c3
https://hal.archives-ouvertes.fr/hal-01886455/file/journal.pone.0193036.pdf
https://hal.archives-ouvertes.fr/hal-01886455/file/journal.pone.0193036.pdf
Autor:
Christopher M. Topham, Jeremy C. Smith
Publikováno v:
Computational Biology and Chemistry. 54:33-43
Relative amino acid residue solvent accessibility values allow the quantitative comparison of atomic solvent-accessible surface areas in different residue types and physical environments in proteins and in protein structural alignments. Geometry-opti
Autor:
Isabelle André, Luce Lozano-Huguet, Christopher M. Topham, Audrey Baylac, Magali Remaud-Simeon, Nathalie Tarrat, Nelly Martineau, Clément Auriol, Hélène Cordier, Marc Maestracci, Marion Stodel, Romain Irague, Clémentine Dressaire, Jean Marie François, Thomas Walther, Yannick Malbert, Robert Huet
Publikováno v:
Nature Communications
Nature Communications, Nature Publishing Group, 2017, 8 (1), pp.15828. ⟨10.1038/ncomms15828⟩
Nature Communications, 2017, 8 (1), pp.15828. ⟨10.1038/ncomms15828⟩
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Nature Communications (8), 12 p.. (2017)
Nature Communications, Nature Publishing Group, 2017, 8 (1), pp.15828. ⟨10.1038/ncomms15828⟩
Nature Communications, 2017, 8 (1), pp.15828. ⟨10.1038/ncomms15828⟩
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Nature Communications (8), 12 p.. (2017)
2,4-Dihydroxybutyric acid (DHB) is a molecule with considerable potential as a versatile chemical synthon. Notably, it may serve as a precursor for chemical synthesis of the methionine analogue 2-hydroxy-4-(methylthio)butyrate, thus, targeting a cons
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0480afe75482467bd655bd42067c686c
https://hal.archives-ouvertes.fr/hal-01608248/document
https://hal.archives-ouvertes.fr/hal-01608248/document
Publikováno v:
Protein Science. 22:1754-1765
The amylosucrase from Neisseria polysaccharea is a transglucosidase from the GH13 family of glycoside-hydrolases that naturally catalyzes the synthesis of α-glucans from the widely available donor sucrose. Interestingly, natural molecular evolution