Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Christopher M, Humphreys"'
Publikováno v:
Virulence, Vol 14, Iss 1 (2023)
ABSTRACTClostridium botulinum, a polyphyletic Gram-positive taxon of bacteria, is classified purely by their ability to produce botulinum neurotoxin (BoNT). BoNT is the primary virulence factor and the causative agent of botulism. A potentially fatal
Externí odkaz:
https://doaj.org/article/a3873da4b3c84f789e1ae81c261b1c92
Autor:
François M. Seys, Christopher M. Humphreys, Claudio Tomi-Andrino, Qi Li, Thomas Millat, Sheng Yang, Nigel P. Minton
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As such, they hol
Externí odkaz:
https://doaj.org/article/acfdb420b5ce4854a65c792a3344a09f
Publikováno v:
Fermentation, Vol 8, Iss 11, p 585 (2022)
Replicating plasmid shuttle vectors are key tools for efficient genetic and metabolic engineering applications, allowing the development of sustainable bioprocesses using non-model organisms with unique metabolic capabilities. To date, very limited g
Externí odkaz:
https://doaj.org/article/e96f1afbade74d9dbd941033d1659ae3
Autor:
Barbara, Bourgade, Christopher M, Humphreys, James, Millard, Nigel P, Minton, M Ahsanul, Islam
Publikováno v:
ACS synthetic biology. 11(5)
The platform chemical ethylene glycol (EG) is used to manufacture various commodity chemicals of industrial importance, but largely remains synthesized from fossil fuels. Although several novel metabolic pathways have been reported for its bioproduct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6fa12e3cf5765909543239cd573266c8
https://pubmed.ncbi.nlm.nih.gov/35543716
https://pubmed.ncbi.nlm.nih.gov/35543716
Autor:
Craig Woods, Christopher M. Humphreys, Claudio Tomi-Andrino, Anne M. Henstra, Michael Köpke, Sean D. Simpson, Klaus Winzer, Nigel P. Minton
Publikováno v:
Applied and Environmental Microbiology. 88
Although microbial genome sequences are relatively easily determined, assigning gene function remains a bottleneck. Consequently, relatively few genes are well characterized, leaving the function of many as either hypothetical or entirely unknown.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c400a7decb9269ce1220daaadbaae972
https://doi.org/10.1128/aem.02479-21
https://doi.org/10.1128/aem.02479-21
Autor:
Anne M. Henstra, Rupert Norman, Florence J. Annan, Sean Dennis Simpson, Klaus Winzer, Michael Köpke, Bakir Al-Sinawi, Nigel P. Minton, Christopher M. Humphreys
Publikováno v:
Applied Microbiology and Biotechnology
Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reporte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28e138646829a318828cad9f2b93d45b
https://doi.org/10.1007/s00253-019-09763-6
https://doi.org/10.1007/s00253-019-09763-6
Autor:
Craig Woods, Michael Köpke, Claudio Tomi-Andrino, Christopher M. Humphreys, Sean Dennis Simpson, Nigel P. Minton, Anne M. Henstra, Klaus Winzer
The majority of the genes present in bacterial genomes remain poorly characterised with up to one third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::353785788756052b86600c367e1a2bc2
https://doi.org/10.1101/2021.05.19.444907
https://doi.org/10.1101/2021.05.19.444907
Publikováno v:
Genes
Genes, Vol 11, Iss 458, p 458 (2020)
Genes, Vol 11, Iss 458, p 458 (2020)
Phenotypic complementation of gene knock-outs is an essential step in establishing function. Here we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele is replaced in situ with a wild type (WT) allele in a p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8398a9f8bb484f12c4f29f3c57707c4c
https://doi.org/10.20944/preprints202003.0321.v1
https://doi.org/10.20944/preprints202003.0321.v1
Publikováno v:
Current Opinion in Biotechnology. 50:174-181
The future sustainable production of chemicals and fuels from non-petrochemical sources, while at the same time reducing greenhouse gas (GHG) emissions, represent two of society's greatest challenges. Microbial chassis able to grow on waste carbon mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7492c331e555dca1b074ac3dc090de6
https://doi.org/10.1016/j.copbio.2017.12.023
https://doi.org/10.1016/j.copbio.2017.12.023
Autor:
Yang Gu, Daphne Groothuis, Christopher M. Humphreys, Nigel P. Minton, Peter Rowe, Patrick Ingle, He Huang, Weihong Jiang, Alan Cockayne, Sarah A. Kuehne
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Understanding the molecular pathogenesis of Clostridioides difficile has relied on the use of ermB-based mutagens in erythromycin-sensitive strains. However, the repeated subcultures required to isolate sensitive variants can lead to the acquisition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e8fec8cf43db35f39ba9fe87b80f4d3
https://doi.org/10.1038/s41598-019-44458-y
https://doi.org/10.1038/s41598-019-44458-y