Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Christopher Larkin"'
Autor:
Christopher Thompson, William K. Wang, Jeong Lee, Michaela Wendeler, Christopher Larkin, Kelly Wilson
Publikováno v:
Biotechnology Progress. 34:1195-1204
This work presents the optimization and critical evaluation of continuous capture chromatography in the downstream process of a recombinant enzyme. For the upstream manufacturing of this molecule, a perfusion process was implemented due to benefits f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5f40951e03e50d165aa79d889910a5c
https://doi.org/10.1002/btpr.2674
https://doi.org/10.1002/btpr.2674
Autor:
Joomi Ahn, John J.G. Tesmer, Wenmin Yuan, Alisa Glukhova, Anna Schwendeman, Louise Chang, Milton J. Axley, Taylor D. Manett, Christopher Larkin, Kelly A. Manthei, James A. Shayman
Publikováno v:
Journal of Biological Chemistry. 292:20313-20327
Lecithin:cholesterol acyltransferase (LCAT) plays a key role in reverse cholesterol transport by transferring an acyl group from phosphatidylcholine to cholesterol, promoting the maturation of high-density lipoproteins (HDL) from discoidal to spheric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6087b227c87c8f1c286d90d862ee1fc
https://doi.org/10.1074/jbc.m117.802736
https://doi.org/10.1074/jbc.m117.802736
Publikováno v:
Journal of immunological methods. 474
Identity testing of biopharmaceutical products is conducted at multiple steps in the manufacturing process, for drug product lot release, and often for product importation. Because of the chemical and structural similarities of antibody-based product
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb865ecbbcf954b071c19235cf614a08
https://pubmed.ncbi.nlm.nih.gov/31525363
https://pubmed.ncbi.nlm.nih.gov/31525363
Autor:
Liangliang Sun, Norman J. Dovichi, Ronald Schoner, Thomas Albanetti, Guijie Zhu, James B. McGivney, Travis Linkous, Christopher Larkin
Publikováno v:
Biotechnology and Bioengineering. 113:2140-2148
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6bf03ade970ff91054d80d12f82fcf8f
https://doi.org/10.1002/bit.25991
https://doi.org/10.1002/bit.25991
Autor:
Christopher Larkin, Santosh Kumar Paidi, James B. McGivney, Ishan Barman, Soumik Siddhanta, Robert Strouse
Publikováno v:
Analytical Chemistry. 88:4361-4368
Product identification is a critical and required analysis for biotheraputics. In addition to regulatory requirements for identity testing on final drug products, in-process identity testing is implemented to reduce business risks associated with fil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a70bec0c6054ca2994e6d9472faf4a4
https://doi.org/10.1021/acs.analchem.5b04794
https://doi.org/10.1021/acs.analchem.5b04794
Autor:
Srilatha Kuntumalla, Guijie Zhu, Hung Yu Lin, James B. McGivney, Christopher Larkin, Norman J. Dovichi, Jennifer Heidbrink-Thompson, Liangliang Sun
Publikováno v:
ELECTROPHORESIS. 37:616-622
We have evaluated CZE-ESI-MS/MS for detection of trace amounts of host cell protein impurities in recombinant therapeutics. Compared to previously published procedures, we have optimized the buffer pH used in the formation of a pH junction to increas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::10a762a53a6d07b7717c81ccca76c797
https://doi.org/10.1002/elps.201500301
https://doi.org/10.1002/elps.201500301
Autor:
Peter L. Mage, H. Tom Soh, Robert Strouse, James B. McGivney, Elizabeth R. Bagley, Michael Eisenstein, Faye M. Walker, Andrew T. Csordas, Margaret A. Nakamoto, Jose Casas-Finet, Christopher Larkin, Emily M. Gruber
Publikováno v:
Analytical chemistry. 90(5)
Biologic drugs are typically manufactured in mammalian host cells, and it is critical from a drug safety and efficacy perspective to detect and remove host cell proteins (HCPs) during production. This is currently achieved with sets of polyclonal ant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3680bf41a200e2d4b413fc4e3c256221
https://pubmed.ncbi.nlm.nih.gov/29436820
https://pubmed.ncbi.nlm.nih.gov/29436820
Autor:
Stephen E. Stein, Jenny Heidbrink Thompson, Sanford P. Markey, Meghan C. Burke, Yuri A. Mirokhin, Christopher Larkin, Dmitrii V. Tchekhovskoi
Publikováno v:
Journal of proteome research. 16(5)
We present a mass spectral library-based method to identify tandem mass spectra of peptides that contain unanticipated modifications and amino acid variants. We describe this as a "hybrid" method because it combines matching both ion m/z and mass los
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98f0f67c7db00c089cc3e709e4667279
https://pubmed.ncbi.nlm.nih.gov/28367633
https://pubmed.ncbi.nlm.nih.gov/28367633
Autor:
Min Zhu, Christopher Larkin, Wai Keen Chung, David Robbins, Nabila Aboulaich, Jenny Heidbrink Thompson
Publikováno v:
Biotechnology Progress
Co-purification of a subset of host cell proteins (HCPs) with monoclonal antibodies (mAbs) during the capture of mAbs on Protein A affinity chromatography is primarily caused by interactions of HCPs with the mAbs. To date, there is limited informatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9daec6b8a1f089e661e7fabb334212c7
https://doi.org/10.1002/btpr.1948
https://doi.org/10.1002/btpr.1948
Autor:
Travis Linkous, Thomas Albanetti, Norman J. Dovichi, Ronald Schoner, James B. McGivney, Christopher Larkin, Zhenbin Zhang
Publikováno v:
Electrophoresis. 38(3-4)
Four methods were compared for analysis of host-cell protein (HCP) impurities in a recombinant mAb. First, CZE-MS/MS was used to analyze the digest of an HCP sample following extraction of the mAb with proteins A and L affinity columns; 220 protein g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1773a628d56fb79825a1896367d6c0f
https://pubmed.ncbi.nlm.nih.gov/27862059
https://pubmed.ncbi.nlm.nih.gov/27862059