Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Christopher John Taylorson"'
Publikováno v:
Analytical Biochemistry. 259:142-151
This paper describes the employment of a novel phenoxy-substituted acridinium ester (di-ortho-bromophenyl-AE) as a chemiluminescent endpoint indicator for ligand binding assays. The reactivity of this compound is such that it is capable of generating
Publikováno v:
Analytical Biochemistry. 251:280-287
A quantitative hybridization technique for the detection of plasmid DNA by the action of a nuclease enzyme is described. The process utilizes the specific capture and detection of a sandwich hybridization, in a microtiter plate, that occurs in a sing
Autor:
Henny J. Eggelte, Stuart Harbron, Brian R. Rabin, Christopher John Taylorson, Antonio Tarragona-Fiol, John M. Ward, E. Sanchez
Publikováno v:
"Protein Engineering, Design and Selection". 6:901-906
In addition to hydrolysing RNA, bovine pancreatic ribonuclease splits esters of pyrimidine nucleoside 3'-phosphates, including dinucleotides. For a series of 3':5'-linked dinucleotides of general structure CpN, where N is a 5' linked nucleoside, kcat
Publikováno v:
Gene. 118:239-245
The coding sequence for the bovine pancreatic ribonuclease (RNase) precursor has been cloned and produced in Escherichia coli using the polymerase chain reaction (PCR) technique. A PCR amplification has been carried out utilizing as template the reco