Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Christopher J. Siebert"'
Autor:
Lee C. Dunn, Harvard W. Morehead, Theodore L. Tisch, Mohsen Navvab, Kenneth W. Talmadge, Christopher J. Siebert, Mohamed Abouelezz, Cheryl Ordunez
Publikováno v:
Journal of Chromatography A. 590:83-91
Three ion-exchange packing materials, Macro-Prep 50 S, Macro-Prep 50 CM and Macro-Prep 50 Q (strong cation, weak cation and strong anion, respectively) were characterized with respect to dynamic protein-binding capacities and chemical stability and r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::056cfb841365c98fe018c6d1a160a002
https://doi.org/10.1016/0021-9673(92)87008-v
https://doi.org/10.1016/0021-9673(92)87008-v
Publikováno v:
Journal of Chromatography A. 587:171-176
A rapid, simple assay for aldehydes generated by oxidation of saccharide units in glycoproteins, using dyes containing hydrazide functionalities, is described. The assay is used, in conjunction with tests of biological activity, to predict oxidation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d2f563204eefe49569baa30ea666bf9
https://doi.org/10.1016/0021-9673(91)85153-7
https://doi.org/10.1016/0021-9673(91)85153-7
Characterization of synthetic macroporous ion-exchange resins in low-pressure cartridges and columns
Autor:
Christopher J. Siebert, Lee C. Dunn, Larry J. Cummings, Mohsen Navvab, Cheryl Ordunez, Kenneth W. Talmadge, Mohamed Aboulezz
Publikováno v:
Journal of Chromatography A. 548:165-178
Three ion-exchange materials and one hydrophobic-interaction chromatography packing, based on a rigid macroporous polymer with large, relatively uniform pores, have been evaluated for low-pressure liquid chromatography of antibodies. These sorbents h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f649612a2c720289a7de66763065ba36
https://doi.org/10.1016/s0021-9673(01)88599-x
https://doi.org/10.1016/s0021-9673(01)88599-x
Publikováno v:
Journal of chromatography. A. 744(1-2)
Capillary polymer sieving electrophoresis using dynamic sieving polymer solutions provides size-based separations of oligonucleotides. The polymer sieving system described here resolves single-stranded oligonucleotides with single-base resolution up
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d96240f478be49edc6476d8c5a75d91a
https://pubmed.ncbi.nlm.nih.gov/8843682
https://pubmed.ncbi.nlm.nih.gov/8843682
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 670:163-169
An uncharged N- hydroxysuccinimide ester derivative of agarose, Affi-Gel 10, exhibited excellent capacity for immobilization, at pH 7.5, of proteins having isoelectric points of 6.5–11.0. Under identical conditions, acidic proteins with isoelectric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fffbe598d054f13eb6df0269d03b20fa
https://doi.org/10.1016/0005-2795(81)90004-0
https://doi.org/10.1016/0005-2795(81)90004-0
Publikováno v:
Journal of Pharmaceutical Sciences. 78:393-398
High-affinity (Ka = 3.0 X 10(8) M-1) monoclonal antidigoxin antibodies were covalently coupled to Sepharose CL-6B, Bio Gel A5m, Affi-gel 15, and agarose:polyacrolein microsphere (APAM) beads. Antibody immobilized to these supports was characterized i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ea0c4276019852e9f71e9423a6c8c17
https://doi.org/10.1002/jps.2600780510
https://doi.org/10.1002/jps.2600780510
Autor:
Lee C. Dunn, Christopher J. Siebert, Larry J. Cummings, Gary S. Ott, J.Keith Duncan, David J. Burke
Publikováno v:
Journal of Chromatography A. 353:425-437
A new anion-exchange material has been developed which allows very rapid resolution of protein mixtures. The Microanalyzer MA7P matrix consists of small (7 micron), spherical, non-porous, polymethacrylate beads with polyethyleneimine (PEI) covalently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26d3f3faa7a67865c90b5e2b8f8f8c54
https://doi.org/10.1016/s0021-9673(01)87113-2
https://doi.org/10.1016/s0021-9673(01)87113-2
Autor:
Ferenc Kilár, Jia-Li Liao, Ming-De Zhu, Stellan Hjertén, Christopher J. Siebert, Karin Elenbring, Albert J.C. Chen
Publikováno v:
Journal of chromatography. 403
A characteristic feature of high-performance electrophoresis (HPE), the electrophoretic counterpart of high-performance liquid chromatography (HPLC), is that the separation chamber is a thin-walled, narrow-bore (0.05-0.3 mm) glass or fused-silica cap
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee2005fa355f5c36affdebcad74f9006
https://pubmed.ncbi.nlm.nih.gov/2445770
https://pubmed.ncbi.nlm.nih.gov/2445770
Publikováno v:
Journal of chromatography. 476
A new affinity column packing for removal of endotoxins has been prepared by coupling USP drug-quality polymyxin B to Affi-Prep, a novel synthetic macroporous polymer. Affi-Prep Polymyxin binds endotoxins from a number of different strains of gram-ne
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bc517f17a698808f4b839c5047a64b4
https://pubmed.ncbi.nlm.nih.gov/2777972
https://pubmed.ncbi.nlm.nih.gov/2777972
Publikováno v:
Journal of chromatography. 397
The performance of a non-porous, anion-exchange packing was evaluated and compared with a number of similar porous high-performance liquid chromatography packings. The non-porous columns were found to be equally efficient for proteins spanning a wide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbbf0a643981290b99d1da9674ec54dc
https://pubmed.ncbi.nlm.nih.gov/3654822
https://pubmed.ncbi.nlm.nih.gov/3654822