Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Christopher J. C. Whitehouse"'
A study was conducted to investigate the desaturation of alkylbenezenes by cytochrome P450BM3 (CYP102A1). It was observed during the study that oxidation of alkylbenzenes with CYP102A1 involves a gamut of P450 activity types that are terminal, sub-te
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04fb07b3ee6805d194cb439d2d9cf88d
https://doi.org/10.1002/chem.200801927
https://doi.org/10.1002/chem.200801927
P450(BM3) (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been extensively studied over a period of almost forty years. The enzyme has been redesigned to catalyse the oxidation of non-natural substrates as diverse as pharmaceutical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0964d68af52e332a9431385646c4e661
https://ora.ox.ac.uk/objects/uuid:b5250b6e-2307-4b36-ade2-b129c43a5251
https://ora.ox.ac.uk/objects/uuid:b5250b6e-2307-4b36-ade2-b129c43a5251
Autor:
Stephen Bell, Benjamin C Rowlatt, Luet-Lok Wong, Anthony J F Strong, Christopher J. C. Whitehouse, Jake A. Yorke
Fatty acid binding and oxidation kinetics for wild type P450(BM3) (CYP102A1) from Bacillus megaterium have been found to display chain length-dependent homotropic behavior. Laurate and 13-methyl-myristate display Michaelis-Menten behavior while there
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48a5b92ad83d46a0418056324a755b28
https://ora.ox.ac.uk/objects/uuid:6c4af262-7dab-4f4a-922d-db73844afc64
https://ora.ox.ac.uk/objects/uuid:6c4af262-7dab-4f4a-922d-db73844afc64
Autor:
Stephen Bell, Richard J. P. Kenny, Henry G. Tufton, Christopher J. C. Whitehouse, Luet-Lok Wong, Lydia C. I. Ogilvie
The evolution of CYP102A1 variants with enhanced activity and altered specificity characteristics.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6d5c6ff652837eb24ecc958ce475c69
https://ora.ox.ac.uk/objects/uuid:455202d9-778e-48b3-a218-c6da5d160852
https://ora.ox.ac.uk/objects/uuid:455202d9-778e-48b3-a218-c6da5d160852
Publikováno v:
ChemInform. 43
P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been extensively studied over a period of almost forty years. The enzyme has been redesigned to catalyse the oxidation of non-natural substrates as diverse as pharmaceuticals,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::85e26883ea1899c48ee0e5c612001b95
https://doi.org/10.1002/chin.201217268
https://doi.org/10.1002/chin.201217268
Autor:
Mark Bartlam, Christopher J. C. Whitehouse, Wen Yang, Jake A. Yorke, Zihe Rao, Stephen Bell, Henry G. Tufton, Lydia C. I. Ogilvie, Luet-Lok Wong, Weihong Zhou
Publikováno v:
Dalton transactions (Cambridge, England : 2003). 40(40)
The substrate-free crystal structure of a five-mutation directed evolution variant of CYP102A1 (P450(BM3)) with generic activity-enhancing properties ("KT2") has been determined to 1.9-Å resolution. There is a close resemblance to substrate-bound st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a4e1f5333d773afe74cd197d2a4f2e6
https://pubmed.ncbi.nlm.nih.gov/21603690
https://pubmed.ncbi.nlm.nih.gov/21603690
Autor:
Christopher J. C. Whitehouse, Mark Bartlam, Benjamin C Rowlatt, Luet-Lok Wong, Anthony J F Strong, Wen Yang, Stephen Bell, Zihe Rao, Jake A. Yorke, Christopher F. Blanford
Publikováno v:
Chembiochem : a European journal of chemical biology. 11(18)
The crystal structures of the haem domains of Ala330Pro and Ile401Pro, two single-site proline variants of CYP102A1 (P450(BM3)) from Bacillus megaterium, have been solved. In the A330P structure, the active site is constricted by the relocation of th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dd3a344676d053ddb383a4872323228
http://ora.ox.ac.uk/objects/uuid:a1e57133-b9e5-4a60-bd0a-d1f9a383fa5d
http://ora.ox.ac.uk/objects/uuid:a1e57133-b9e5-4a60-bd0a-d1f9a383fa5d
Publikováno v:
Chemistry (Weinheim an der Bergstrasse, Germany). 17(24)
The oxidation of o-xylene by P450(BM3) from Bacillus megaterium yields, in addition to the products formed by microsomal P450s, two metabolites containing an NIH-shifted methyl group, one of which lacks the aromatic character of the substrate. The fa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d6454f1c75f6c6b1c66c1a8f0092552
https://pubmed.ncbi.nlm.nih.gov/21523836
https://pubmed.ncbi.nlm.nih.gov/21523836
Autor:
Anthony J F Strong, Wen Yang, Jake A. Yorke, Luet Lok Wong, Mark Bartlam, Stephen Bell, Edward J. Morse, Zihe Rao, Christopher F. Blanford, Christopher J. C. Whitehouse
Publikováno v:
Chembiochem : a European journal of chemical biology. 10(10)
The power of proline: Bold amino acid substitutions in sensitive protein regions are frequently unproductive, while more subtle mutations can be sufficient to bring about dramatic changes. But introducing proline at the residue next to the sulfur lig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c648a0656d93a2c8321dbead3aec7d3a
https://pubmed.ncbi.nlm.nih.gov/19492389
https://pubmed.ncbi.nlm.nih.gov/19492389
Publikováno v:
The Ubiquitous Roles of Cytochrome P450 Proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa86d0ffd8f40dfba16b2c98e530184e
https://doi.org/10.1002/9780470028155.ch14
https://doi.org/10.1002/9780470028155.ch14