Výsledky vyhledávání - "Christopher J. Noren"

Akademický článek

Various mutations compensate for a deleterious lacZα insert in the replication enhancer of M13 bacteriophage.

Autor: Emily M Zygiel, Karen A Noren, Marta A Adamkiewicz, Richard J Aprile, Heather K Bowditch, Christine L Carroll, Maria Abigail S Cerezo, Adelle M Dagher, Courtney R Hebert, Lauren E Hebert, Gloria M Mahame, Stephanie C Milne, Kelly M Silvestri, Sara E Sutherland, Alexandria M Sylvia, Caitlyn N Taveira, David J VanValkenburgh, Christopher J Noren, Marilena Fitzsimons Hall

Publikováno v: PLoS ONE, Vol 12, Iss 4, p e0176421 (2017)

M13 and other members of the Ff class of filamentous bacteriophages have been extensively employed in myriad applications. The Ph.D. series of phage-displayed peptide libraries were constructed from the M13-based vector M13KE. As a direct descendent

Externí odkaz: https://doaj.org/article/705e4724753e4fb193c657c6c9d87199

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Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA

Autor: Lynne Apone, Shaohua Li, Bradley W. Langhorst, Xiaoli Sun, Lixin Chen, Christopher J. Noren, Ming-Qun Xu, Laurence Ettwiller, Aihua Zhang

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports

The use of next-generation sequencing (NGS) has been instrumental in advancing biological research and clinical diagnostics. To fully utilize the power of NGS, complete, uniform coverage of the entire genome is required. In this study, we identified

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4e514d22d25a4b180221086b0dffba6
http://link.springer.com/article/10.1038/s41598-018-34079-2

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Mapping of polyglutamylation in tubulins using nanoLC-ESI-MS/MS

Autor: Ulla Hansen, Christopher J. Noren, Hang Gyeong Chin, Sriharsa Pradhan, Colleen McClung, Cristian I. Ruse

Publikováno v: Analytical Biochemistry. 612:113761

Tubulin polyglutamylation is a polymeric modification that extends from the carboxyl-terminus of tubulins. Molecular description of amino acids and their branching polyglutamyls is a hallmark of tubulin in microtubules. There are different chemical a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::104de8c4c500b109e652ac6e360d5b70
https://doi.org/10.1016/j.ab.2020.113761

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Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries

Autor: Lauren E. Hebert, Kieu T.H. Nguyen, Emily M. Zygiel, Christopher J. Noren, Karen A. Noren, Marta A. Adamkiewicz, Carola B. Meléndez-Ríos, Holly R. Boyle, Marilena Fitzsimons Hall, Christina M. Martone

Publikováno v: Analytical Biochemistry. 462:35-43

A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of a propagation advantage exhibited by the phage clone displaying the peptide. We previously characterized HAIYPRH, from the M13-based Ph.D.-7 phage displa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d36dd8d47cbbd979b0cc89534affdac4

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Single M13 bacteriophage tethering and stretching

Autor: Angela M. Belcher, Ahmad S. Khalil, Ricardo R. Brau, Jorge Ferrer, Christopher J. Noren, Matthew J. Lang, Stephen T. Kottmann

Publikováno v: Proceedings of the National Academy of Sciences. 104:4892-4897

The ability to present biomolecules on the highly organized structure of M13 filamentous bacteriophage is a unique advantage. Where previously this viral template was shown to direct the orientation and nucleation of nanocrystals and materials, here

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d94914086e547eb61fcfdea477a8442
https://doi.org/10.1073/pnas.0605727104

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Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

Autor: Corinna Tuckey, Christopher J. Noren, Cristen Peterson, Na Ke, Mehmet Berkmen, Thomas J. Mansell, Alana Szkodny, Michael Paul Robinson, Paul A. Colussi, Paul Riggs, Christopher H. Taron, Matthew P. DeLisa, Julie Lobstein

Publikováno v: Nature Communications

Current methods for producing immunoglobulin G (IgG) antibodies in engineered cells often require refolding steps or secretion across one or more biological membranes. Here, we describe a robust expression platform for biosynthesis of full-length IgG

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13c424707ae17b70f2004fe62d6140ec
https://doi.org/10.1038/ncomms9072

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Genetically encoded fragment-based discovery of glycopeptide ligands for carbohydrate-binding proteins

Autor: Oksana Gerlits, Beth M. Paschal, Simon Ng, Ping Zhang, Lara K. Mahal, Pavel I. Kitov, Amika Sood, Brian Kasper, Leighton Coates, Christopher J. Noren, Lu Deng, Robert J. Woods, John S. Klassen, Chang-Chun Ling, Katrina F. Tjhung, Ratmir Derda, Edith Y. Lin

Publikováno v: Journal of the American Chemical Society. 137(16)

We describe an approach to accelerate the search for competitive inhibitors for carbohydrate-recognition domains (CRDs). Genetically encoded fragment-based-discovery (GE-FBD) uses selection of phagedisplayed glycopeptides to dock a glycan fragment at

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b168a69d5e33a1084790a430c0353ac2
https://pubmed.ncbi.nlm.nih.gov/25860443

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Biochemical characterization of a Naegleria TET-like oxygenase and its application in single molecule sequencing of 5-methylcytosine

Autor: Zhiyi Sun, Lana Saleh, Shengxi Guan, Jurate Bitinaite, Richard J. Roberts, Ivan R. Corrêa, Alexey Fomenkov, Romualdas Vaisvila, Christopher J. Noren, Esta Tamanaha, Xiaodong Cheng, Yu Zheng, Nan Dai, June E. Pais

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 112(14)

Modified DNA bases in mammalian genomes, such as 5-methylcytosine ( 5m C) and its oxidized forms, are implicated in important epigenetic regulation processes. In human or mouse, successive enzymatic conversion of 5m C to its oxidized forms is carried

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a2664560765a1337e5857ef14b7c6ef
https://pubmed.ncbi.nlm.nih.gov/25831492

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Multivalent Site-Specific Phage Modification Enhances the Binding Affinity of Receptor Ligands

Autor: Jaymes Beech, Joel Linden, Heidi Figler, Lana Saleh, Julie Frentzel, Ivan R. Corrêa, Christopher J. Noren, Brenda Baker, Melissa A. Marshall, Kimberly A. Kelly, Caroline Ramspacher, Siva Sai Krishna Dasa

High-throughput screening of combinatorial chemical libraries is a powerful approach for identifying targeted molecules. The display of combinatorial peptide libraries on the surface of bacteriophages offers a rapid, economical way to screen billions

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00ccd043a81d7578137f022021581643
https://europepmc.org/articles/PMC6252250/

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