GTP binding to translation factor eIF2B stimulates its guanine nucleotide exchange activity

Autor: Christopher J. Kershaw, Martin D. Jennings, Francesco Cortopassi, Margherita Guaita, Hawra Al-Ghafli, Graham D. Pavitt

Publikováno v: iScience, Vol 24, Iss 12, Pp 103454- (2021)

Summary: eIF2B is the guanine nucleotide exchange factor (GEF) required for cytoplasmic protein synthesis initiation in eukaryotes and its regulation within the integrated stress response (ISR). It activates its partner factor eIF2, thereby promoting

Externí odkaz: https://doaj.org/article/63576347261d43b39ec105640f9c4851

Interaction of the La-related protein Slf1 with colliding ribosomes maintains translation of oxidative-stress responsive mRNAs

Autor: Martin D Jennings, Priya Srivastava, Christopher J Kershaw, David Talavera, Christopher M Grant, Graham D Pavitt

Publikováno v: Nucleic Acids Research.

In response to oxidative stress cells reprogram gene expression to enhance levels of antioxidant enzymes and promote survival. In Saccharomyces cerevisiae the polysome-interacting La-related proteins (LARPs) Slf1 and Sro9 aid adaptation of protein sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3af404b8973844e50e1fb43a1e6aaa2c
https://doi.org/10.1093/nar/gkad272

Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes

Autor: Martin D Jennings, Christopher J Kershaw, Tomas Adomavicius, Graham D Pavitt

Publikováno v: eLife, Vol 6 (2017)

Phosphorylation of eIF2α controls translation initiation by restricting the levels of active eIF2-GTP/Met-tRNAi ternary complexes (TC). This modulates the expression of all eukaryotic mRNAs and contributes to the cellular integrated stress response.

Externí odkaz: https://doaj.org/article/75f8611e47d04d95b6a8f582950959d1

The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response.

Autor: Christopher J Kershaw, Joseph L Costello, Lydia M Castelli, David Talavera, William Rowe, Paul F G Sims, Mark P Ashe, Simon J Hubbard, Graham D Pavitt, Chris M Grant

Publikováno v: PLoS Genetics, Vol 11, Iss 1, p e1004903 (2015)

The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p and Sro9p are atypical-La motif containing proteins which are members of a superfamily of RNA-binding proteins conserved in eukaryotes.

Externí odkaz: https://doaj.org/article/b192b29bdbf44b96b098f4262be44c89

Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma

Autor: Christopher J. Kershaw, Michael Hölzel, Richard Lisle, Ze'ev Ronai, Thomas Tüting, Paola Falletta, Tatjana Sauka-Spengler, Amy Kenyon, Colin R. Goding, Maike Effern, Rasmus Freter, Anne E. Willis, Mark R. Middleton, Robert Siddaway, Xin Lu, Luis Sánchez-del-Campo, Graham D. Pavitt, Jagat Chauhan, Matthew J. Daniels, Francesca M. Buffa

Publikováno v: Falletta, P, Sanchez-del-Campo, L, Chauhan, J, Effern, M, Kenyon, A, Kershaw, C J, Siddaway, R, Lisle, R J, Freter, R, Daniels, M J, Lu, X, Tüting, T, Middleton, M, Buffa, F M, Willis, A E, Pavitt, G D, Ronai, Z A, Sauka-Spengler, T, Hölzel, M & Goding, C R 2017, ' Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma. ', Genes and development, vol. 31, no. 1, pp. 18-33 . https://doi.org/10.1101/gad.290940.116

The intratumor microenvironment generates phenotypically distinct but interconvertible malignant cell subpopulations that fuel metastatic spread and therapeutic resistance. Whether different microenvironmental cues impose invasive or therapy-resistan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65856e223778261b0e8b4d266238c47d
https://doi.org/10.1101/gad.290940.116