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pro vyhledávání: '"Christopher D. Wassman"'
Autor:
Özlem Demir, Roberta Baronio, Faezeh Salehi, Christopher D Wassman, Linda Hall, G Wesley Hatfield, Richard Chamberlin, Peter Kaiser, Richard H Lathrop, Rommie E Amaro
Publikováno v:
PLoS Computational Biology, Vol 7, Iss 10, p e1002238 (2011)
The tumor suppressor protein p53 can lose its function upon single-point missense mutations in the core DNA-binding domain ("cancer mutants"). Activity can be restored by second-site suppressor mutations ("rescue mutants"). This paper relates the fun
Externí odkaz:
https://doaj.org/article/13916c5e1ca2495eb6707700eecd1972
Autor:
Peter K. Kaiser, Rommie E. Amaro, A. Richard Chamberlin, Brad D. Wallentine, Faezeh Salehi, Dawei Lin, Hartmut Luecke, Chiung-Kuang Chen, Roberta Baronio, Christopher D. Wassman, Linda V. Hall, Özlem Demir, Richard H. Lathrop, Benjamin P. Chung, G. Wesley Hatfield
Publikováno v:
Nature Communications
The tumour suppressor p53 is the most frequently mutated gene in human cancer. Reactivation of mutant p53 by small molecules is an exciting potential cancer therapy. Although several compounds restore wild-type function to mutant p53, their binding s
Publikováno v:
Wassman, Christopher D.; Tam, Phillip Y.; Lathrop, Richard H.; & Weiss, Gregory A.(2004). Predicting oligonucleotide-directed mutagenesis failures in protein engineering. Nucleic Acids Research, 32(21), 6407-6413. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1n20z9x4
Wassman, CD; Tam, PY; Lathrop, RH; & Weiss, GA. (2004). Predicting oligonucleotide-directed mutagenesis failures in protein engineering. Nucleic Acids Research, 32(21), 6407-6413. doi: 10.1093/nar/gkh977. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/27v66496
Wassman, CD; Tam, PY; Lathrop, RH; & Weiss, GA. (2004). Predicting oligonucleotide-directed mutagenesis failures in protein engineering. Nucleic Acids Research, 32(21), 6407-6413. doi: 10.1093/nar/gkh977. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/27v66496
Protein engineering uses oligonucleotide-directed mutagenesis to modify DNA sequences through a two-step process of hybridization and enzymatic synthesis. Inefficient reactions confound attempts to introduce mutations, especially for the construction
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc73938cffbe64f460c738e1c159343b
http://www.escholarship.org/uc/item/1n20z9x4
http://www.escholarship.org/uc/item/1n20z9x4
Autor:
Linda V. Hall, Rommie E. Amaro, Richard Chamberlin, Richard H. Lathrop, G. Wesley Hatfield, Özlem Demir, Faezeh Salehi, Peter K. Kaiser, Roberta Baronio, Christopher D. Wassman
Publikováno v:
PLoS Computational Biology
Demir, Özlem; Baronio, Roberta; Salehi, Faezeh; Wassman, Christopher D; Hall, Linda; Hatfield, G Wesley; et al.(2011). Ensemble-based computational approach discriminates functional activity of p53 cancer and rescue mutants.. PLoS computational biology, 7(10), e1002238-e10022e1002238. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/4nz7j4vb
PLoS Computational Biology, Vol 7, Iss 10, p e1002238 (2011)
Demir, Özlem; Baronio, Roberta; Salehi, Faezeh; Wassman, Christopher D; Hall, Linda; Hatfield, G Wesley; et al.(2011). Ensemble-based computational approach discriminates functional activity of p53 cancer and rescue mutants.. PLoS computational biology, 7(10), e1002238-e10022e1002238. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/4nz7j4vb
PLoS Computational Biology, Vol 7, Iss 10, p e1002238 (2011)
The tumor suppressor protein p53 can lose its function upon single-point missense mutations in the core DNA-binding domain (“cancer mutants”). Activity can be restored by second-site suppressor mutations (“rescue mutants”). This paper relates
Publikováno v:
International Journal of Bioinformatics Research and Applications. 4:324
Gene synthesis is hampered by two obstacles: improper assembly of oligonucleotides; oligonucleotide defects incurred during chemical synthesis. To overcome the first problem, we describe the employment of a Computationally Optimised DNA Assembly (COD