At-line high throughput site-specific glycan profiling using targeted mass spectrometry

Autor: Bertie Chi, Christel Veyssier, Toyin Kasali, Faisal Uddin, Christopher A. Sellick

Publikováno v: Biotechnology Reports, Vol 25, Iss , Pp - (2020)

Protein post-translational modification (PTM) plays an important role in many biological processes; of which glycosylation is arguably one of the most complex and diverse modifications and is crucial for the safety and efficacy of biotherapeutic prot

Externí odkaz: https://doaj.org/article/e6765dcd5c884f75931396c9cec65727

Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin

Autor: Richard Turner, Christopher A. Sellick, Hubert Schwarz, Johan Rockberg, Raymond Field, Magdalena Malm, Paul Varley, Ye Zhang, Veronique Chotteau, Caijuan Zhan

Publikováno v: Journal of Biotechnology. 309:44-52

Process intensification in mammalian cell culture-based recombinant protein production has been achieved by high cell density perfusion exceeding 10(8) cells/mL in the recent years. As the majority ...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc81170e887409cbb16129b51311f0be
https://doi.org/10.1016/j.jbiotec.2019.12.017

Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production

Autor: Jenny Heidbrink Thompson, Richard Turner, Tingting Cui, Toyin Kasali, Christopher A. Sellick, Bertie Chi

Publikováno v: Journal of biotechnology. 305

Monoclonal antibody (mAb) fragmentation is a well-known degradation pathway that results in product loss and can significantly impact product quality, efficacy, or even cause immunogenic reactions, thus potentially endangering patients’ health. It

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3fc9df5bdc08fe673a4e2017be213cd
https://pubmed.ncbi.nlm.nih.gov/31442501

Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single-Cell Level

Autor: Christopher Mark Smales, Guglielmo Rosignoli, Andrew Smith, Claire Pearce, Christopher A. Sellick, Eva Pekle

Publikováno v: Biotechnology journal. 14(7)

Biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells requires the generation of high-producing clonal cell lines. During cell line development, cell cloning using fluorescence-activated cell sorting (FACS) has the potential to comb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414ab5a873d3e867ccbfb654d0ed9c53
https://pubmed.ncbi.nlm.nih.gov/30925020

Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness

Autor: Arfa Maqsood, Alexandra S. Croxford, Royston Goodacre, Christopher A. Sellick, Gill Stephens, Hans V. Westerhoff, Alan J. Dickson

Publikováno v: Biotechnology Journal, 10(9), 1434-1445. Wiley-VCH Verlag
Sellick, C A, Croxford, A S, Maqsood, A R, Stephens, G M, Westerhoff, H V, Goodacre, R & Dickson, A J 2015, ' Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness ', Biotechnology Journal, vol. 10, no. 9, pp. 1434-1445 . https://doi.org/10.1002/biot.201400664
Sellick, C A, Croxford, A S, Maqsood, A R, Stephens, G M, Westerhoff, H V, Goodacre, R & Dickson, A J 2015, ' Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness ', Biotechnology Journal
, vol. 10, no. 9, pp. 1434-1445 . https://doi.org/10.1002/biot.201400664

Whilst development of medium and feeds has provided major advances in recombinant protein production in CHO cells, the fundamental understanding is limited. We have applied metabolite profiling with established robust (GC-MS) analytics to define the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cd49745facca2abbd7f2cca626bebf2
https://doi.org/10.1002/biot.201400664