The topology of DNA entrapment by cohesin rings

Autor: Christophe Chapard, Robert Jones, Johanna C. Scheinost, K. A. Nasmyth, van Oepen T

SummaryCohesin entraps sister DNAs within tripartite rings created by pairwise interactions between Smc1,Smc3, and Scc1. Because the ATPase heads of Smc1 and Smc3 can interact with each other, cohesin rings in fact have the potential to form a variet

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed659f7b80efa9f2a5620d0063999066
https://doi.org/10.1101/495762

Multiple interactions between Scc1 and Scc2 activate cohesin’s DNA dependent ATPase and replace Pds5 during loading

Autor: Byung-Gil Lee, Kim Nasmyth, Sotaro Kikuchi, Hongtao Yu, Wentao Chen, Eeson Rajendra, Jan Löwe, Jean Metson, Naomi J Petela, Madhusudhan Srinivisan, Thomas G. Gligoris, Bin Hu, Menelaos Voulgaris, Christophe Chapard

SummaryIn addition to sharing with condensin an ability to organize DNA into chromatids, cohesin regulates enhancer-promoter interactions and confers sister chromatid cohesion. Association with chromosomes is regulated by hook-shaped HEAT repeat prot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61a43883ee5e2612689895638b8dbe7c
https://doi.org/10.1101/205914

Biological chromodynamics: a general method for measuring protein occupancy across the genome by calibrating ChIP-seq

Autor: Bin, Hu, Naomi, Petela, Alexander, Kurze, Kok-Lung, Chan, Christophe, Chapard, Kim, Nasmyth

Publikováno v: Nucleic Acids Research

Sequencing DNA fragments associated with proteins following in vivo cross-linking with formaldehyde (known as ChIP-seq) has been used extensively to describe the distribution of proteins across genomes. It is not widely appreciated that this method m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::85074d564f09ad4be6743a5bcc7f2305
https://pubmed.ncbi.nlm.nih.gov/26130708

Cohesin regulates homology search during recombinational DNA repair

Autor: Jérôme Savocco, Fabien Girard, Romain Koszul, Agnès Dumont, Hélène Bordelet, Agnès Thierry, Aurèle Piazza

Publikováno v: Nature Cell Biology
Nature Cell Biology, 2021, 23 (11), pp.1176-1186. ⟨10.1038/s41556-021-00783-x⟩
Nature Cell Biology, Nature Publishing Group, 2021, 23 (11), pp.1176-1186. ⟨10.1038/s41556-021-00783-x⟩
bioRxiv
Biorxiv

SummaryHomologous recombination (HR) is a ubiquitous DNA double-strand break (DSB) repair mechanism that promotes cell survival. It entails a potentially genome-wide homology search step, carried out along a conserved RecA/Rad51-ssDNA nucleoprotein f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ce38c9b218b7ad34aee8a541ea893c4
https://hal.science/hal-03427219

Regulation of Cohesin-Mediated Chromosome Folding by Eco1 and Other Partners

Autor: Romain Koszul, Luciana Lazar-Stefanita, Olivier Gadal, Nathalie Bastié, Agnès Thierry, Rémi Montagne, Lise Dauban, Axel Cournac, Frédéric Beckouët

Publikováno v: Molecular Cell
Molecular Cell, Elsevier, 2020, 77 (6), pp.1279-1293.e4. ⟨10.1016/j.molcel.2020.01.019⟩
Molecular Cell, 2020, 77 (6), pp.1279-1293.e4. ⟨10.1016/j.molcel.2020.01.019⟩

International audience; Cohesin, a member of the SMC complex family, holds sister chromatids together but also shapes chromosomes by promoting the formation of long-range intra-chromatid loops, a process proposed to be mediated by DNA loop extrusion.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::806de202e2e9f177d5d8e071b71481ac
https://hal.archives-ouvertes.fr/hal-03064572