Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Christoph J Wienken"'
Autor:
Karolina Corin, Philipp Baaske, Deepali B Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Sandra Geissler, Christoph J Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Shuguang Zhang
Publikováno v:
PLoS ONE, Vol 6, Iss 10, p e23036 (2011)
Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E. coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immuno
Externí odkaz:
https://doaj.org/article/08442d96fbdd466ca1606d4e76038eda
Autor:
Karolina Corin, Philipp Baaske, Deepali B Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Christoph J Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Yuan Luo, Dieter Braun, Shuguang Zhang
Publikováno v:
PLoS ONE, Vol 6, Iss 11, p e25067 (2011)
A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid productio
Externí odkaz:
https://doaj.org/article/1b4d966cc2c14c76a1c8cf17b5b8e4f9
Autor:
Moran Jerabek-Willemsen, Susanne A. I. Seidel, Christoph J. Wienken, Sandra Geissler, Dieter Braun, Dirk Trauner, Stefan Duhr, Philipp Baaske, Alwin Reiter
Publikováno v:
Angewandte Chemie (International Ed. in English)
Look, no label! Microscale thermophoresis makes use of the intrinsic fluorescence of proteins to quantify the binding affinities of ligands and discriminate between binding sites. This method is suitable for studying binding interactions of very smal
Autor:
Sandra Geissler, Stefan Duhr, Moran Jerabek-Willemsen, Philipp Baaske, Susanne A. I. Seidel, Alwin Reiter, Christoph J. Wienken, Dieter Braun, Dirk Trauner
Publikováno v:
Angewandte Chemie. 124:10810-10814
Publikováno v:
Angewandte Chemie. 122:2286-2290
Publikováno v:
Angewandte Chemie International Edition. 49:2238-2241
Quantification of biomolecular binding reactions in theirnative environment is crucial for biology and medicine.However, reliable methods are rare. We have developed anew immobilization-free method in which thermophoresis,the movement of molecules in
Autor:
Dieter Braun, Philipp Baaske, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Shuguang Zhang, Xiaoqiang Wang, Karolina Corin
Two major bottlenecks in elucidating the structure and function of membrane proteins are the difficulty of producing large quantities of functional receptors, and stabilizing them for a sufficient period of time. Selecting the right surfactant is thu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c7ca68b3617db99072efcc585885ab9
https://europepmc.org/articles/PMC3107261/
https://europepmc.org/articles/PMC3107261/
Autor:
Christoph J. Wienken, Philipp Baaske, Sandra Geissler, Stefan Duhr, Shuguang Zhang, Karolina Corin, Dieter Braun
Publikováno v:
Nature Publishing Group
Scientific Reports
Scientific Reports
The vomeronasal system is one of several fine-tuned scent-detecting signaling systems in mammals. However, despite significant efforts, how these receptors detect scent remains an enigma. One reason is the lack of sufficient purified receptors to per
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::467e2a856855a2570c265483f887bced
http://hdl.handle.net/1721.1/88274
http://hdl.handle.net/1721.1/88274
Autor:
Moran Jerabek-Willemsen, Karolina Corin, Christoph J. Wienken, Deepali Ravel, Junyao Song, Dieter Braun, Xiaoqiang Wang, Sandra Geissler, Stefan Duhr, Shuguang Zhang, Philipp Baaske, Emily J. Brown
Publikováno v:
PLoS ONE
PLoS
PLoS ONE, Vol 6, Iss 10, p e23036 (2011)
PLoS
PLoS ONE, Vol 6, Iss 10, p e23036 (2011)
Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoa
Autor:
Deepali Ravel, Karolina Corin, Christoph J. Wienken, Moran Jerabek-Willemsen, Dieter Braun, Yuan Luo, Philipp Baaske, Junyao Song, Emily J. Brown, Stefan Duhr, Shuguang Zhang, Xiaoqiang Wang
Publikováno v:
PLoS ONE, Vol 6, Iss 11, p e25067 (2011)
PLoS ONE
PLoS
PLoS ONE
PLoS
A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid productio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e57f4e06718713fd147f6ce101d57f3