Zobrazeno 1 - 10
of 124
pro vyhledávání: '"Christoph Eckerskorn"'
Publikováno v:
ELECTROPHORESIS. 31:1754-1763
Since its introduction five decades ago, free-flow electrophoresis (FFE) has been mainly employed for the isolation and fractionation of cells, cell organelles and protein mixtures. In the meantime, the growing interest in the proteome of these bio-p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8682fabc29ca2aef52699068bf0f0600
https://doi.org/10.1002/elps.200900771
https://doi.org/10.1002/elps.200900771
Autor:
Monika Hauptmann, Robert Wildgruber, Ute Sukop, Christian Obermaier, Gerhard Weber, Mikkel Nissum, Johan Malmström, Christoph Eckerskorn, Sabine Kuhfuss
Publikováno v:
PROTEOMICS. 7:4218-4227
Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3460b0170b06e852f82f1f9ab6800369
https://doi.org/10.1002/pmic.200700166
https://doi.org/10.1002/pmic.200700166
Publikováno v:
ELECTROPHORESIS. 25:1735-1747
We have elaborated a protocol for the fractionation of both hydrophilic and hydrophobic proteins using as a model the matrix and membrane compartments of highly purified rat liver peroxisomes because of their distinct proteomes and characteristic com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e980ee7cc9c9bdbad2549be7df80be1
https://doi.org/10.1002/elps.200305834
https://doi.org/10.1002/elps.200305834
Autor:
Friedrich Lottspeich, Kristin Bauer, Jörg Hagmann, Christoph Eckerskorn, Marcus Otte, Wolfgang Siess, Michael A. A. Kratzer, Georg J. Arnold, Karin Luber de Quintana
Publikováno v:
Blood. 96:4236-4245
A 38-kd protein that associates with F-actin structures in activated platelets and endothelial cells was purified, cloned, and characterized. The protein contains an N-terminal PDZ motif, a large intervening sequence, and a C-terminal LIM domain and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b42b0abed0018fcae433059f4031f292
https://doi.org/10.1182/blood.v96.13.4236
https://doi.org/10.1182/blood.v96.13.4236
Publikováno v:
Electrophoresis. 21:2209-2218
The human plasma protein patterns obtained by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is a good model system for post-translational modifications because of the existence of several "ladders" of protein spots [Anderson, N. L., A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c8bcd0422560788d9d60b906c05a7fe
https://doi.org/10.1002/1522-2683(20000601)21:11<2209::aid-elps2209>3.0.co
https://doi.org/10.1002/1522-2683(20000601)21:11<2209::aid-elps2209>3.0.co
Autor:
Maria Luiza Vilela Oliva, Christoph Eckerskorn, Misako U. Sampaio, Jose Carlos Souza-Pinto, Mariana S. Araujo, Reini Mentele, Isabel de Fátima Correia Batista, Vera Lucia Flor Silveira, Claudio A. M. Sampaio, Ennes A. Auerswald
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1477:64-74
A serine proteinase inhibitor isolated from Leucaena leucocephala seeds (LlTI) was purified to homogeneity by acetone fractionation, ion exchange chromatography, gel filtration and reverse phase chromatography (HPLC). SDS-PAGE indicated a protein wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5651720f9fb42fb0391dc247cf41dd69
https://doi.org/10.1016/s0167-4838(99)00285-x
https://doi.org/10.1016/s0167-4838(99)00285-x
Publikováno v:
Planta. 209:478-486
Delta(5)-3beta-Etaydroxysteroid dehydrogenase (Delta(5)-3beta-HSD; EC 1.1.1.145), an enzyme converting pregn-5-ene-3beta-ol-20-one (pregnenolone) to pregn-5-ene-3,20-dione (isoprogesterone), was isolated from the soluble fraction of suspension-cultur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::166cc6d0270610837ce7e671544eb8ce
https://doi.org/10.1007/s004250050751
https://doi.org/10.1007/s004250050751
Publikováno v:
FEBS Letters. 456:68-72
The molecular cloning of the first subunit C of the plant vacuolar H + -ATPase is reported. Tonoplast vesicles were purified from barley leaves by sucrose gradient centrifugation, and the tonoplast polypeptides were separated by two-dimen- sional (2-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::662a1a89781cece7fcb69d0c30327c17
https://doi.org/10.1016/s0014-5793(99)00937-0
https://doi.org/10.1016/s0014-5793(99)00937-0
Autor:
Christoph Eckerskorn, Joachim Rassow, Frank Gärtner, Heiko Martin, F. Lottspeich, Nikolaus Pfanner
Publikováno v:
Analytical Biochemistry. 265:123-128
We have developed a protocol for the sequential release of the intermembrane space (IMS) content of Saccharomyces cerevisiae mitochondria. Two distinct fractions were obtained: a soluble IMS with cytochrome b2 as key marker and a salt-extractable IMS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c791f398d7b9f7882bf7971359e7676
https://doi.org/10.1006/abio.1998.2863
https://doi.org/10.1006/abio.1998.2863
Publikováno v:
Journal of Bacteriology. 180:5997-6004
Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a82556d17559da8bf782109ca324821
https://doi.org/10.1128/jb.180.22.5997-6004.1998
https://doi.org/10.1128/jb.180.22.5997-6004.1998