Generation and Comparative Analysis of an

Autor: Ganesh, Warthi, Jessica L, Faulkner, Jaser, Doja, Amr R, Ghanam, Pan, Gao, Allison C, Yang, Orazio J, Slivano, Candee T, Barris, Taylor C, Kress, Scott D, Zawieja, Susan H, Griffin, Xiaoling, Xie, Alan, Ashworth, Christine K, Christie, William B, Bryant, Ajay, Kumar, Michael J, Davis, Xiaochun, Long, Lin, Gan, Eric J Belin, de Chantemèle, Qing, Lyu, Joseph M, Miano

Publikováno v: Nat Cardiovasc Res

All current smooth muscle cell (SMC) Cre mice similarly recombine floxed alleles in vascular and visceral SMCs. Here, we present an Itga8-CreER(T2) knock-in mouse and compare its activity with a Myh11-CreER(T2) mouse. Both Cre drivers demonstrate equ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2df126c8dc2db1aa9eabb92959c61c62
https://pubmed.ncbi.nlm.nih.gov/36424917

CRISPR-Cas9–Mediated Epitope Tagging Provides Accurate and Versatile Assessment of Myocardin—Brief Report

Autor: Yu Han, Sachin A. Gupte, Bing Guo, Qiangzong Yin, Vidhi Dhagia, Paul Herring, Christine K. Christie, Orazio J. Slivano, Joseph M. Miano, Xiaochun Long, Mary E. Wines-Samuelson, Qing Lyu

Publikováno v: Arteriosclerosis, Thrombosis, and Vascular Biology. 38:2184-2190

Objective— Unreliable antibodies often hinder the accurate detection of an endogenous protein, and this is particularly true for the cardiac and smooth muscle cofactor, MYOCD (myocardin). Accordingly, the mouse Myocd locus was targeted with 2 indep

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b0c39849cd7b26d7c75c5bda17214798
https://doi.org/10.1161/atvbaha.118.311171

CRISPR-Cas9 Genome Editing of a Single Regulatory Element Nearly Abolishes Target Gene Expression in Mice—Brief Report

Autor: Albert W. Cheng, Christine K. Christie, Orazio J. Slivano, Yu Han, Joseph M. Miano

Publikováno v: Arteriosclerosis, Thrombosis, and Vascular Biology. 35:312-315

Objective— To ascertain the importance of a single regulatory element in the control of Cnn1 expression using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing. Approach and Results�

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66d7bd9caab22b3cd32ce5e427208502
https://doi.org/10.1161/atvbaha.114.305017

Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice

Autor: Dick Tibboel, Wei Yan, Rutger W W Brouwer, Tono Djuwantono, Qing Lyu, Alice S. Brooks, Erwin Brosens, Alan J. Burns, Karen L. de Mesy Bentley, Joke B. G. M. Verheij, Hans J. Stoop, Suowen Xu, Christine K. Christie, Maria M. Alves, Joseph M. Miano, Daniel Oliver, Orazio J. Slivano, Vivek Nanda, Wilfred F. J. van IJcken, Zheng Gen Jin, Michael Doukas, Michael P. Wilson, Danny Halim, Robert M.W. Hofstra, Yu Han

Publikováno v: Proceedings of the National Academy of Sciences of the U.S.A., 114(13), E2739-E2747. National Academy of Sciences
Proceedings of the National Academy of Science of the United States of America, 114(13), E2739-E2747. NATL ACAD SCIENCES

Megacystis microcolon intestinal hypoperistalsis syndrome (MMIHS) is a congenital visceral myopathy characterized by severe dilation of the urinary bladder and defective intestinal motility. The genetic basis of MMIHS has been ascribed to spontaneous

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dc548a35984283da87acebfc178921d
https://pure.eur.nl/en/publications/4acfe589-9d42-4512-97f7-5fce3f8c06cb