CHOmics: A web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines.

Autor: Dongdong Lin, Hima B Yalamanchili, Xinmin Zhang, Nathan E Lewis, Christina S Alves, Joost Groot, Johnny Arnsdorf, Sara P Bjørn, Tune Wulff, Bjørn G Voldborg, Yizhou Zhou, Baohong Zhang

Publikováno v: PLoS Computational Biology, Vol 16, Iss 12, p e1008498 (2020)

Chinese hamster ovary (CHO) cell lines are widely used in industry for biological drug production. During cell culture development, considerable effort is invested to understand the factors that greatly impact cell growth, specific productivity and p

Externí odkaz: https://doaj.org/article/849744a569d14e8f84cf44db421cb425

CHO genome mining for synthetic promoter design

Autor: Chapman Wright, Yusuf B. Johari, Rashmi Kshirsagar, David C. James, Scott Estes, Adam J. Brown, Christina S. Alves, Yizhou Zhou

Publikováno v: Journal of Biotechnology. 294:1-13

Synthetic promoters are an attractive alternative for use in mammalian hosts such as CHO cells as they can be designed de novo with user-defined functionalities. In this study, we describe and validate a method for bioprocess-directed design of synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a371f7d7bea22a82cb66ef50271390
https://doi.org/10.1016/j.jbiotec.2019.01.015

Benchmarking and optimization of a high-throughput sequencing based method for transgene sequence variant analysis in biotherapeutic cell line development

Autor: Christina S. Alves, Chongfeng Xu, Yizhou Zhou, Baohong Zhang, Justin Crisafulli, Eric Marshall, Patrick Cullen, Fergal Casey, Thomas M. Carlile, Chao Sun, Dongdong Lin, Joost Groot

Publikováno v: Biotechnology journalREFERENCES. 16(8)

In recent years High-Throughput Sequencing (HTS) based methods to detect mutations in biotherapeutic transgene products have become a key quality step deployed during the development of manufacturing cell line clones. Previously we reported on a high

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c7ec4f571cfa5cc295a3e29b246dc19
https://pubmed.ncbi.nlm.nih.gov/34018310

CHOmics: A web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines

Autor: Nathan E. Lewis, Dongdong Lin, Hima Yalamanchili, Christina S. Alves, Johnny Arnsdorf, Yizhou Zhou, Baohong Zhang, Tune Wulff, Xinmin Zhang, Sara Peterson Bjørn, Bjørn G. Voldborg, Joost Groot

Publikováno v: PLoS computational biology, vol 16, iss 12
PLoS Computational Biology
Lin, D, Yalamanchili, H B, Zhang, X, Lewis, N E, Alves, C S, Groot, J, Arnsdorf, J, Bjørn, S P, Wulff, T, Voldborg, B G, Zhou, Y & Zhang, B 2020, ' CHOmics : A web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines ', PLOS Computational Biology, vol. 16, no. 12, e1008498 . https://doi.org/10.1371/journal.pcbi.1008498
PLoS Computational Biology, Vol 16, Iss 12, p e1008498 (2020)

Chinese hamster ovary (CHO) cell lines are widely used in industry for biological drug production. During cell culture development, considerable effort is invested to understand the factors that greatly impact cell growth, specific productivity and p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce535c630d3b5c1781f87633a7e721c
https://escholarship.org/uc/item/7kn8h9gx

PDGF suppresses the sulfation of CD44v and potentiates CD44v-mediated binding of colon carcinoma cells to fibrin under flow.

Autor: Christina S Alves, Konstantinos Konstantopoulos

Publikováno v: PLoS ONE, Vol 7, Iss 10, p e41472 (2012)

Fibrin(ogen) mediates sustained tumor cell adhesion and survival in the pulmonary vasculature, thereby facilitating the metastatic dissemination of tumor cells. CD44 is the major functional fibrin receptor on colon carcinoma cells. Growth factors, su

Externí odkaz: https://doaj.org/article/6559e23e0d4448aa96911e02fe31863e