Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Christin Pohl"'
Autor:
Christin Pohl, Gregory Effantin, Eaazhisai Kandiah, Sebastian Meier, Guanghong Zeng, Werner Streicher, Dorotea Raventos Segura, Per H. Mygind, Dorthe Sandvang, Line Anker Nielsen, Günther H. J. Peters, Guy Schoehn, Christoph Mueller-Dieckmann, Allan Noergaard, Pernille Harris
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Here the authors report the cryo-EM structure of a triple-mutant of the anti-microbial peptide plectasin, PPI42, assembling in a pH- and concentration dependent manner into helical non-amyloid fibrils. The fibrils formation is reversible, and follows
Externí odkaz:
https://doaj.org/article/40dae761724f4f05a632e7c5544c619d
Autor:
Diana C. Rodriguez Camargo, Sean Chia, Joseph Menzies, Benedetta Mannini, Georg Meisl, Martin Lundqvist, Christin Pohl, Katja Bernfur, Veronica Lattanzi, Johnny Habchi, Samuel IA Cohen, Tuomas P. J Knowles, Michele Vendruscolo, Sara Linse
Publikováno v:
Frontiers in Molecular Biosciences, Vol 8 (2021)
The aggregation of the human islet amyloid polypeptide (IAPP) is associated with diabetes type II. A quantitative understanding of this connection at the molecular level requires that the aggregation mechanism of IAPP is resolved in terms of the unde
Externí odkaz:
https://doaj.org/article/06a8b25e7ff84e83a621b38a48768838
Autor:
Alina Kulakova, Dillen Augustijn, Inas El Bialy, Lorenzo Gentiluomo, Maria Laura Greco, Stefan Hervø-Hansen, Sowmya Indrakumar, Sujata Mahapatra, Marcello Martinez Morales, Christin Pohl, Marco Polimeni, Aisling Roche, Hristo L. Svilenov, Andreas Tosstorff, Matja Zalar, Robin Curtis, Jeremy P. Derrick, Wolfgang Frieß, Alexander P. Golovanov, Mikael Lund, Allan Nørgaard, Tarik A. Khan, Günther H. J. Peters, Alain Pluen, Dierk Roessner, Werner W. Streicher, Christopher F. van der Walle, Jim Warwicker, Shahid Uddin, Gerhard Winter, Jens Thostrup Bukrinski, Åsmund Rinnan, Pernille Harris
Publikováno v:
Molecular Pharmaceutics.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b30e33ef72e37b5def8fabb536c102a3
https://doi.org/10.1021/acs.molpharmaceut.3c00013
https://doi.org/10.1021/acs.molpharmaceut.3c00013
Autor:
Werner Streicher, Sujata Mahapatra, Günther H.J. Peters, Allan Nørgaard, Pernille Harris, Christin Pohl, Alina Kulakova
Publikováno v:
Pohl, C, Mahapatra, S, Kulakova, A, Streicher, W, Peters, G H J, Nørgaard, A & Harris, P 2022, ' Combination of high throughput and structural screening to assess protein stability-A screening perspective ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 171 . https://doi.org/10.1016/j.ejpb.2021.08.018
European journal of pharmaceutics and biopharmaceutics 171, 1-10 (2022). doi:10.1016/j.ejpb.2021.08.018
Pohl, C, Mahapatra, S, Kulakova, A, Streicher, W, Peters, G H J, Nørgaard, A & Harris, P 2022, ' Combination of high throughput and structural screening to assess protein stability – A screening perspective ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 171, pp. 1-10 . https://doi.org/10.1016/j.ejpb.2021.08.018
European journal of pharmaceutics and biopharmaceutics 171, 1-10 (2022). doi:10.1016/j.ejpb.2021.08.018
Pohl, C, Mahapatra, S, Kulakova, A, Streicher, W, Peters, G H J, Nørgaard, A & Harris, P 2022, ' Combination of high throughput and structural screening to assess protein stability – A screening perspective ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 171, pp. 1-10 . https://doi.org/10.1016/j.ejpb.2021.08.018
European journal of pharmaceutics and biopharmaceutics 171, 1 - 10 (2022). doi:10.1016/j.ejpb.2021.08.018
High throughput screening for measuring the stability of industrially relevant proteins and their variants is necessary for quality assessm
High throughput screening for measuring the stability of industrially relevant proteins and their variants is necessary for quality assessm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea860997e4adeb3ebbd6a386757edca3
Autor:
Allan Noergaard, Christin Pohl, Christoph Mueller-Dieckmann, Pernille Harris, Sebastian Meier, Grégory Effantin, Guy Schoehn, Günther H.J. Peters, Eaazhisai Kandiah, Guanghong Zeng, Werner Streicher
Self-assembly and fibril formation play important roles in protein behavior. Amyloid fibrils formation is well-studied due to its role in neurodegenerative diseases and characterized by refolding of the protein into predominant β-sheet form. However
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ff79836725c75bbf81dd79e113438ae
https://doi.org/10.1101/2021.09.06.458672
https://doi.org/10.1101/2021.09.06.458672
Autor:
Werner Streicher, Christin Pohl, Alexander P. Golovanov, Pernille Harris, Matja Zalar, Sowmya Indrakumar, Nuska Tschammer, Allan Nørgaard, Günther H.J. Peters
Publikováno v:
Indrakumar, S, Zalar, M, Tschammer, N, Pohl, C A, Nørgaard, A, Streicher, W, Harris, P, Golovanov, A P & Peters, G H J 2021, ' Development of a Fast Screening Method for Selecting Excipients in Formulations using MD simulations, NMR and MicroScale Thermophoresis ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 158, pp. 11-20 . https://doi.org/10.1016/j.ejpb.2020.10.015
Development of peptide therapeutics generally involves screening of excipients that inhibit peptide-peptide interactions, hence aggregation, and improve peptide stability. We used the therapeutic peptide plectasin to develop a fast screening method t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a53ed78bec97c51dee0259e918874e4
https://orbit.dtu.dk/en/publications/4305e7c3-4649-4809-9959-fe8436ce07c3
https://orbit.dtu.dk/en/publications/4305e7c3-4649-4809-9959-fe8436ce07c3
Autor:
Shahid Uddin, Christin Pohl, Christopher F. van der Walle, Inas El Bialy, Jeremy P. Derrick, Andreas Tosstorff, Robin Curtis, Sujata Mahapatra, Werner Streicher, Dillen Augustijn, Aisling Roche, Alina Kulakova, Wolfgang Frieß, Åsmund Rinnan, Hristo L. Svilenov, Dierk Roessner, Lorenzo Gentiluomo, Sowmya Indrakumar, Alain Pluen, Gerhard Winter, Pernille Harris, Allan Nørgaard, Günther H.J. Peters, Maria Laura Greco, Marcello Martinez Morales, Tarik A. Khan
Publikováno v:
Molecular Pharmaceutics
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Vitaliyivna Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Frieß, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Friess, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Vitaliyivna Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Frieß, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Friess, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Therapeutic protein candidates should exhibit favorable properties that render them suitable to become drugs. Nevertheless, there are no well-established guidelines for the efficient selection of proteinaceous molecules with desired features during e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414939ada22b81281c984e3f808bda3d
Autor:
Werner Streicher, Christin Pohl, Pernille Harris, Matja Zalar, Günther H.J. Peters, Allan Nørgaard, Sowmya Indrakumar, Alexander P. Golovanov
Publikováno v:
Indrakumar, S, Zalar, M, Pohl, C, Nørgaard, A, Streicher, W, Harris, P, Golovanov, A P & Peters, G H J 2019, ' Conformational Stability Study of a Therapeutic Peptide Plectasin Using Molecular Dynamics Simulations in Combination with NMR ', Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, vol. 123, no. 23, pp. 4867-4877 . https://doi.org/10.1021/acs.jpcb.9b02370
The Journal of Physical Chemistry B
Indrakumar, S, Zalar, M, Pohl, C, Nørgaard, A, Streicher, W, Harris, P, Golovanov, A P & Peters, G H J 2019, ' Conformational Stability Study of a Therapeutic Peptide Plectasin Using Molecular Dynamics Simulations in Combination with NMR ', The Journal of Physical Chemistry Part B . https://doi.org/10.1021/acs.jpcb.9b02370
The Journal of Physical Chemistry B
Indrakumar, S, Zalar, M, Pohl, C, Nørgaard, A, Streicher, W, Harris, P, Golovanov, A P & Peters, G H J 2019, ' Conformational Stability Study of a Therapeutic Peptide Plectasin Using Molecular Dynamics Simulations in Combination with NMR ', The Journal of Physical Chemistry Part B . https://doi.org/10.1021/acs.jpcb.9b02370
Plectasin is a small, cysteine-rich peptide antibiotic which belongs to the class of antimicrobial peptides and has potential antibacterial activity against various Gram-positive bacteria. In the current study, the effect of pH and ionic strength (Na
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b14acb1f23ea6f33800a4d1bda45a96
https://pubmed.ncbi.nlm.nih.gov/31099578
https://pubmed.ncbi.nlm.nih.gov/31099578
Autor:
Åsmund Rinnan, Werner Streicher, Alina Kulakova, Hristo L. Svilenov, Sujata Mahapatra, Dillen Augustijn, Christin Pohl
Publikováno v:
Augustijn, D, Mahapatra, S, Streicher, W, Svilenov, H, Kulakova, A, Pohl, C & Rinnan, Å 2019, ' Novel non-linear curve fitting to resolve protein unfolding transitions in intrinsic fluorescence differential scanning fluorimetry ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 142, pp. 506-517 . https://doi.org/10.1016/j.ejpb.2019.06.001
European Journal of Pharmaceutics and Biopharmaceutics
Augustijn, D, Mahapatra, S, Streicher, W, Svilenov, H, Kulakova, A, Pohl, C & Rinnan, Å 2019, ' Novel Non-linear Curve Fitting to Resolve Protein Unfolding Transitions in Intrinsic Fluorescence Differential Scanning Fluorimetry ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 142, pp. 506-517 . https://doi.org/10.1016/j.ejpb.2019.06.001
European Journal of Pharmaceutics and Biopharmaceutics
Augustijn, D, Mahapatra, S, Streicher, W, Svilenov, H, Kulakova, A, Pohl, C & Rinnan, Å 2019, ' Novel Non-linear Curve Fitting to Resolve Protein Unfolding Transitions in Intrinsic Fluorescence Differential Scanning Fluorimetry ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 142, pp. 506-517 . https://doi.org/10.1016/j.ejpb.2019.06.001
In biotherapeutic protein research, an estimation of the studied protein’s thermal stability is one of the important steps that determine developability as a function of solvent conditions. Differential Scanning Fluorimetry (DSF) can be applied to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc5ba02489bbe425ce7d88ba23af6863
https://curis.ku.dk/portal/da/publications/novel-nonlinear-curve-fitting-to-resolve-protein-unfolding-transitions-in-intrinsic-fluorescence-differential-scanning-fluorimetry(60971cf8-4f9f-48ac-b36b-adb3001143b2).html
https://curis.ku.dk/portal/da/publications/novel-nonlinear-curve-fitting-to-resolve-protein-unfolding-transitions-in-intrinsic-fluorescence-differential-scanning-fluorimetry(60971cf8-4f9f-48ac-b36b-adb3001143b2).html
Autor:
Günther H.J. Peters, Sowmya Indrakumar, Alexander P. Golovanov, Pernille Harris, Inas El Bialy, Werner Streicher, Matja Zalar, Allan Noergaard, Wolfgang Friess, Christin Pohl
Publikováno v:
Molecular Pharmaceutics
Pohl, C A, Zalar, M, Bialy, I E, Indrakumar, S, Peters, G H J, Friess, W, Golovanov, A P, Streicher, W W, Noergaard, A & Harris, P 2020, ' The Effect of Point Mutations on the Biophysical Properties of an Antimicrobial Peptide : Development of a Screening Protocol for Peptide Stability Screening ', Molecular Pharmaceutics, vol. 17, no. 9, pp. 3298-3313 . https://doi.org/10.1021/acs.molpharmaceut.0c00406
Pohl, C A, Zalar, M, Bialy, I E, Indrakumar, S, Peters, G H J, Friess, W, Golovanov, A P, Streicher, W W, Noergaard, A & Harris, P 2020, ' The Effect of Point Mutations on the Biophysical Properties of an Antimicrobial Peptide : Development of a Screening Protocol for Peptide Stability Screening ', Molecular Pharmaceutics, vol. 17, no. 9, pp. 3298-3313 . https://doi.org/10.1021/acs.molpharmaceut.0c00406
Therapeutic peptides and proteins show enormous potential in the pharmaceutical market, but high costs in discovery and development are limiting factors so far. Single or multiple point mutations are commonly introduced in protein drugs to increase t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dacc5f6de055b16d70fda2e85673a8c