Výsledky vyhledávání - "Christilyn P Graff"

Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library

Autor: Robert W. Siegel, Yik Andy Yeung, Peter Heinzelman, James R. Coleman, Christilyn P Graff, Lee K. Opresko, H. Steven Wiley, Jennifer R. Cochran, K. Dane Wittrup, David W. Colby, Michael J. Feldhaus, Jeffrey S. Swers, Jane M. Weaver Feldhaus

Publikováno v: Nature Biotechnology. 21:163-170

A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb8d834cab315446b8045484a6c1711f
https://doi.org/10.1038/nbt785

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Engineering antibody affinity by yeast surface display

Autor: David W, Colby, Brenda A, Kellogg, Christilyn P, Graff, Yik A, Yeung, Jeffrey S, Swers, K Dane, Wittrup

Publikováno v: Methods in enzymology. 388

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b3a175f25a2cd1e30fd43270bf6ca236
https://pubmed.ncbi.nlm.nih.gov/15289082

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Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C

Autor: K. Dane Wittrup, Kerry A. Chester, Richard H. J. Begent, Christilyn P Graff

Publikováno v: Protein engineering, designselection : PEDS. 17(4)

An scFv has been engineered to bind carcinoembryonic antigen (CEA) with a dissociation half-time >4 days at 37 degrees C. Two mutations responsible for this affinity increase were isolated by screening yeast surface-displayed mutant libraries by flow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93a0050f933ede972f65161b70b441dc
https://pubmed.ncbi.nlm.nih.gov/15115853

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Engineering Antibody Affinity by Yeast Surface Display

Autor: Yik Andy Yeung, Christilyn P Graff, K. Dane Wittrup, Jeffrey S. Swers, Brenda A. Kellogg, David W. Colby

Publisher Summary This chapter elaborates the engineering antibody affinity by yeast surface display (YSD). YSD is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. The methods for disp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0d3a91cbe0253072d199b78ba41f399c
https://doi.org/10.1016/s0076-6879(04)88027-3

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Theoretical analysis of antibody targeting of tumor spheroids: importance of dosage for penetration, and affinity for retention

Autor: Christilyn P, Graff, K Dane, Wittrup

Publikováno v: Cancer research. 63(6)

The interplay among antibody/antigen binding kinetics, antibody diffusion, and antigen metabolic turnover together determines the depth of penetration of antitumor antibodies into prevascular tumor spheroid cell clumps. A sharp boundary between an ou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::76c913adc6f2aa72e8aed9166726e084
https://pubmed.ncbi.nlm.nih.gov/12649189

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