Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Christian Schrøder Kaas"'
Autor:
Xiaoli Xu, Guisheng Zeng, Meagan Hamblin, Yue Wang, Rebecca S. Shapiro, James E. DiCarlo, Alexey V. Revtovich, Caroline B. M. Porter, Christian Schrøder Kaas, George M. Church, Natalia V. Kirienko, James J. Collins, Alejandro Chavez
Publikováno v:
Nature microbiology
Candida albicans is the leading cause of fungal infections; yet, complex genetic interaction analysis remains cumbersome in this diploid pathogen. Here, we developed a CRISPR-Cas9-based ‘gene drive array’ (GDA) platform to facilitate efficient ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9e08e301ffb81be660d602357adb149
https://doi.org/10.1038/s41564-017-0043-0
https://doi.org/10.1038/s41564-017-0043-0
Autor:
Ryan Cecchi, Christian Schrøder Kaas, Santiago Lopez Garnier, Yingleong Chan, Xiaoge Guo, George M. Church, Alejandro Chavez, Angela Tung, James J. Collins, James E. DiCarlo, Eric D. Kelsic, Max G. Schubert
Construction of genetic variant libraries with phenotypic measurement is central to advancing today’s functional genomics, and remains a grand challenge. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::785fd2e95c21fbe0b990a36013742124
https://doi.org/10.1101/195776
https://doi.org/10.1101/195776
Autor:
Xiaoge Guo, James J. Collins, Angela Tung, George M. Church, Ryan J Cecchi, Christian Schrøder Kaas, Yingleong Chan, Yi Yin, James E. DiCarlo, Max G. Schubert, Eric D. Kelsic, Alejandro Chavez, Santiago Lopez Garnier
Publikováno v:
Nature biotechnology
Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b46ef1e34a3f777a771161df5be999f
https://pubmed.ncbi.nlm.nih.gov/29786095
https://pubmed.ncbi.nlm.nih.gov/29786095
Autor:
Julian Brandl, Mikael Rørdam Andersen, Helene Faustrup Kildegaard, Claus Kristensen, Anne Mathilde Lund, Lasse Ebdrup Pedersen, Christian Schrøder Kaas
Publikováno v:
BMC Systems Biology
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', B M C Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', BMC Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', B M C Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', BMC Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4
Background Protein secretion is one of the most important processes in eukaryotes. It is based on a highly complex machinery involving numerous proteins in several cellular compartments. The elucidation of the cell biology of the secretory machinery
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94006e7c43ddde148668ebe5d0603d29
https://doi.org/10.1186/s12918-017-0414-4
https://doi.org/10.1186/s12918-017-0414-4
Autor:
Mikael Rørdam Andersen, Claus Kristensen, Helene Faustrup Kildegaard, Christian Schrøder Kaas, Dietmar Weilguny, Yuzhou Fan
Publikováno v:
Pharmaceutical Bioprocessing. 2:437-448
Bioprocessing of the important Chinese hamster ovary (CHO) cell lines used for the production of biopharmaceuticals stands at the brink of several redefining events. In 2011, the field entered the genomics era, which has accelerated omics-based pheno
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0cbc9d7fd7387359be40df907e1020e6
https://doi.org/10.4155/pbp.14.54
https://doi.org/10.4155/pbp.14.54
Publikováno v:
BMC Genomics
Kaas, C S, Kristensen, C, Betenbaugh, M J & Andersen, M R 2015, ' Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy ', B M C Genomics, vol. 16, no. 160 . https://doi.org/10.1186/s12864-015-1391-x
Kaas, C S, Kristensen, C, Betenbaugh, M J & Andersen, M R 2015, ' Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy ', B M C Genomics, vol. 16, no. 160 . https://doi.org/10.1186/s12864-015-1391-x
Background The DHFR negative CHO DXB11 cell line (also known as DUX-B11 and DUKX) was historically the first CHO cell line to be used for large scale production of heterologous proteins and is still used for production of a number of complex proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f73fd6542ba489425ec6087096dd796b
http://europepmc.org/articles/PMC4359788
http://europepmc.org/articles/PMC4359788
Autor:
Gert Bolt, Jens Jacob Hansen, Christian Schrøder Kaas, Mikael Rørdam Andersen, Claus Kristensen
Publikováno v:
Kaas, C S, Bolt, G, Hansen, J J, Andersen, M R & Kristensen, C 2015, ' Deep sequencing reveals different compositions of mRNA transcribed from the F8 gene in a panel of FVIII-producing CHO cell lines ', Biotechnology Journal, vol. 10, no. 7, pp. 1082-1089 . https://doi.org/10.1002/biot.201400667
Coagulation factor VIII (FVIII) is one of the most complex biopharmaceuticals due to the large size, poor protein stability and extensive post-translational modifications. As a consequence, efficient production of FVIII in mammalian cells poses a maj
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1acb3c78e4c2218a8000e048f5d589c1
https://orbit.dtu.dk/en/publications/9c8c6758-0da6-42f9-bbd4-a8b4230291b9
https://orbit.dtu.dk/en/publications/9c8c6758-0da6-42f9-bbd4-a8b4230291b9
Autor:
Kelley M. Heffner, Deniz Baycin-Hizal, Michael J. Betenbaugh, Christian Schrøder Kaas, Amit Kumar
Publikováno v:
Cell Engineering ISBN: 9783319103198
The genetic sequencing of Chinese hamster ovary cells has initiated a systems biology era for biotechnology applications. In addition to genomics, critical ‘omics data sets also include proteomics, transcriptomics and metabolomics. Recently, the us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f7ba8fef324328275cc6d5c593f367c6
https://doi.org/10.1007/978-3-319-10320-4_19
https://doi.org/10.1007/978-3-319-10320-4_19
Autor:
Anne Mathilde Lund, Christian Schrøder Kaas, Helene Faustrup Kildegaard, Claus Kristensen, Mikael Rørdam Andersen
Publikováno v:
Lund, A M, Kaas, C S, Kildegaard, H F, Kristensen, C & Andersen, M R 2013, ' RNA-seq based expression analysis of the CHO cell protein secretion pathway ' 3rd Annual Next Generation Sequencing Asia Congress, Singapore, Singapore, 08/10/2013-09/10/2013, .
Technical University of Denmark Orbit
Technical University of Denmark Orbit
The Chinese hamster ovary (CHO) cell-line is the predominant mammalian industrial cell line being used to produce recombinant therapeutic proteins. Although CHO cells have been used for more than 25 years, the genome sequence was first published in 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::da0cef51381b435992c3fdfa3ce3a147
http://orbit.dtu.dk/en/publications/rnaseq-based-expression-analysis-of-the-cho-cell-protein-secretion-pathway(11526c30-1839-4e41-9edf-dfc09c0e9eeb).html
http://orbit.dtu.dk/en/publications/rnaseq-based-expression-analysis-of-the-cho-cell-protein-secretion-pathway(11526c30-1839-4e41-9edf-dfc09c0e9eeb).html
Autor:
Lizbeth Hedstrom, Jens Christian Frisvad, Jakob Blæsbjerg Nielsen, Xin E. Sun, Christian Schrøder Kaas, Uffe Hasbro Mortensen, Hans Jasper Genee, Bjarne Gram Hansen
Publikováno v:
Hansen, B G, Sun, X E, Genee, H J, Kaas, C S, Nielsen, J B, Frisvad, J C & Hedstrom, L 2012, ' Adaptive evolution of drug targets in producer and non-producer organisms ', Biochemical Journal, vol. 441, no. 1, pp. 219-226 . https://doi.org/10.1042/BJ20111278
MPA (mycophenolic acid) is an immunosuppressive drug produced by several fungi in Penicillium subgenus Penicillium. This toxic metabolite is an inhibitor of IMPDH (IMP dehydrogenase). The MPA-biosynthetic cluster of Penicillium brevicompactum contain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c562d6271efa1340c75029ec15542412
https://pubmed.ncbi.nlm.nih.gov/21916847
https://pubmed.ncbi.nlm.nih.gov/21916847