Sustained delivery of the bone morphogenetic proteins BMP-2 and BMP-7 for cartilage repair and regeneration in osteoarthritis

Autor: Ciara Whitty, Christian Pernstich, Charlotte Marris, Andrew McCaskie, Michael Jones, Frances Henson

Publikováno v: Osteoarthritis and Cartilage Open, Vol 4, Iss 1, Pp 100240- (2022)

Objective: Attempts to utilise growth factors (GF) such as bone morphogenic proteins (BMPs) to treat osteoarthritis (OA) in the clinic have not secured widespread adoption. However, the novel crystalline GF formulation called PODS offers new perspect

Externí odkaz: https://doaj.org/article/712a594bd389472f8e8230903c42a495

Sustained Neurotrophin Release from Protein Nanoparticles Mediated by Matrix Metalloproteinases Induces the Alignment and Differentiation of Nerve Cells

Autor: Yuka Matsuzaki, Rina Maruta, Keiko Takaki, Eiji Kotani, Yasuko Kato, Ryoichi Yoshimura, Yasuhisa Endo, Ciara Whitty, Christian Pernstich, Raj Gandhi, Michael Jones, Hajime Mori

Publikováno v: Biomolecules, Vol 9, Iss 10, p 510 (2019)

The spatial and temporal availability of cytokines, and the microenvironments this creates, is critical to tissue development and homeostasis. Creating concentration gradients in vitro using soluble proteins is challenging as they do not provide a se

Externí odkaz: https://doaj.org/article/2163037a146d44baa5ede03fb9200469

Sustained Neurotrophin Release from Protein Nanoparticles Mediated by Matrix Metalloproteinases Induces the Alignment and Differentiation of Nerve Cells

Autor: Yasuhisa Endo, Keiko Takaki, Raj Gandhi, Eiji Kotani, Ryoichi Yoshimura, Yasuko Kato, Rina Maruta, Matsuzaki Yuka, Christian Pernstich, Michael Jones, Hajime Mori, Ciara Whitty

Publikováno v: Biomolecules
Biomolecules, Vol 9, Iss 10, p 510 (2019)
Volume 9
Issue 10

The spatial and temporal availability of cytokines, and the microenvironments this creates, is critical to tissue development and homeostasis. Creating concentration gradients in vitro using soluble proteins is challenging as they do not provide a se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fa0d963d1d675df43a73e4f8a76c079
http://europepmc.org/articles/PMC6843502

DNA looping by FokI: the impact of twisting and bending rigidity on protein-induced looping dynamics

Autor: Stephen E. Halford, David A. Rusling, Ineke Brouwer, Niels Laurens, Christian Pernstich, Gijs J.L. Wuite

Publikováno v: Laurens, N, Rusling, D A, Pernstich, C, Brouwer, I, Halford, S E & Wuite, G J L 2012, ' DNA looping by FokI: the impact of twisting and bending rigidity on protein-induced looping dynamics ', Nucleic Acids Research, vol. 40, no. 11, pp. 4988-4997 . https://doi.org/10.1093/nar/gks184
Nucleic Acids Research, 40(11), 4988-4997
Nucleic Acids Research
Nucleic Acids Research, 40(11), 4988-4997. Oxford University Press

Protein-induced DNA looping is crucial for many genetic processes such as transcription, gene regulation and DNA replication. Here, we use tethered-particle motion to examine the impact of DNA bending and twisting rigidity on loop capture and release

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2999bb8b391aa747b6743fa011c456c1
https://research.vu.nl/en/publications/9367e63f-d619-46b1-bc04-0598654cf152

Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes

Autor: Kara van Aelst, Christian Pernstich, Kayarat Saikrishnan, Mahesh Kumar Chand, Neha Nirwan, Manasi Kulkarni, Mark D. Szczelkun, Fiona M. Diffin

Publikováno v: Chand, M K, Nirwan, N, Diffin, F M, van Aelst, K, Kulkarni, M, Pernstich, C, Szczelkun, M D & Saikrishnan, K 2015, ' Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes ', Nature Chemical Biology, vol. 11, pp. 870-877 . https://doi.org/10.1038/nchembio.1926
Nature chemical biology

Endonucleolytic double-strand DNA break production requires separate strand cleavage events. Although catalytic mechanisms for simple dimeric endonucleases are available, there are many complex nuclease machines which are poorly understood in compari

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c15cb90b723d81554205d1ebf06b2ea
https://research-information.bris.ac.uk/ws/files/47930130/15402_2_supp_196134_nt8ftt_convrt.pdf