Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Christian Ostermeier"'
Autor:
Roger M Benoit, Christian Ostermeier, Martin Geiser, Julia Su Zhou Li, Hans Widmer, Manfred Auer
Publikováno v:
PLoS ONE, Vol 11, Iss 4, p e0153158 (2016)
Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids. The efficiency of co-transformation cloning is howeve
Externí odkaz:
https://doaj.org/article/5bd0bf043a6f454f9a90f2d8e0a4cdef
Autor:
Andreas F. Haag, Christian Ostermeier
Publikováno v:
BioTechniques, Vol 46, Iss 6, Pp 453-457 (2009)
We describe the development of a novel positive-selection vector, RHP-AmpS, that is suitable for seamless cloning and high-level protein expression in Escherichia coli. In this vector, β-lactamase (Bla) was rendered nonfunctional by replacing the co
Externí odkaz:
https://doaj.org/article/c278540c2d8a44dd96849052c773bab7
Autor:
Christopher P Landowski, Anne Huuskonen, Ramon Wahl, Ann Westerholm-Parvinen, Anne Kanerva, Anna-Liisa Hänninen, Noora Salovuori, Merja Penttilä, Jari Natunen, Christian Ostermeier, Bernhard Helk, Juhani Saarinen, Markku Saloheimo
Publikováno v:
PLoS ONE, Vol 10, Iss 8, p e0134723 (2015)
The filamentous fungus Trichoderma reesei has tremendous capability to secrete proteins. Therefore, it would be an excellent host for producing high levels of therapeutic proteins at low cost. Developing a filamentous fungus to produce sensitive ther
Externí odkaz:
https://doaj.org/article/ed9e1da5f3cc47a1ad32b7da38c7f35f
Autor:
Ujjini H. Manjunatha, Natko Nuber, Cynthia L. Chappell, Wilbur H. Chen, Rajiv S. Jumani, Jay Parthiban Lakshman, Richa Chandra, Natasha Aziz, Dean Wetty, Thierry T. Diagana, Johanne Blais, Hanns-Christian Tillmann, Florencia Segal, Christian Ostermeier
Publikováno v:
ACS Infectious Diseases
Cryptosporidiosis is a leading cause of moderate-to-severe diarrhea in low- and middle-income countries, responsible for high mortality in children younger than two years of age, and it is also strongly associated with childhood malnutrition and grow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8baea2ea3108dd8cfa33ea2b1aa8ae2c
https://doi.org/10.1021/acsinfecdis.1c00057
https://doi.org/10.1021/acsinfecdis.1c00057
Autor:
Christian Ostermeier, Hartmut Michel
Publikováno v:
Transition Metals in Microbial Metabolism ISBN: 9781003211129
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c4bae8e54bc65a8ba0310f995acf2818
https://doi.org/10.1201/9781003211129-11
https://doi.org/10.1201/9781003211129-11
Autor:
Julia Klopp, Daniela Scherer-Becker, Aurélie Winterhalter, Christian Ostermeier, Rémy Gébleux, Alvar D. Gossert
Publikováno v:
Journal of biomolecular NMR. 71(4)
We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13C-methyl methionine labeling in rich media, where yields of up to a g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f0ff2350436e5c3de6cde8dee65474d
https://pubmed.ncbi.nlm.nih.gov/29779067
https://pubmed.ncbi.nlm.nih.gov/29779067
Publikováno v:
Professional Development in Education. 38:377-394
The study reported in this article draws upon data collected for the programme ‘Increasing the Efficiency of Mathematics and Science instruction’ (SINUS-Transfer), a professional development project in Germany. This programme’s approach require
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eabbf3518bc463c831c5d52a882935a8
https://doi.org/10.1080/19415257.2011.626582
https://doi.org/10.1080/19415257.2011.626582
Autor:
Rene Hemmig, Patrick Graff, Nicole-Claudia Meisner, Régis Cèbe, Manfred Auer, Roger Benoit, Hans Widmer, Joerg Kallen, Christian Ostermeier
Publikováno v:
Journal of Molecular Biology. 397:1231-1244
Hu-antigen R (HuR) is a ubiquitous RNA-binding protein that comprises three RNA recognition motifs (RRMs). The first two tandem RRMs are known to bind to AU-rich elements (AREs) in the 3'-untranslated region of many mRNAs. The third RRM is connected
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bddbb6ed21a31467216ad5a57bf30268
https://doi.org/10.1016/j.jmb.2010.02.043
https://doi.org/10.1016/j.jmb.2010.02.043
Autor:
Martin Klumpp, Michael Habig, David Beer, Sigmar Dressler, Viral Patel, Anke Blechschmidt, Christian Ostermeier, Andreas Billich, Barbara Hess
Publikováno v:
SLAS Discovery. 14:679-689
High-throughput screening often identifies not only specific, stoichiometrically binding inhibitors but also undesired com- pounds that unspecifically interfere with the targeted activity by nonstoichiometrically binding, unfolding, and/or inactivat-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74e76fb77ae7cddc198e91427e7912a1
https://doi.org/10.1177/1087057109336586
https://doi.org/10.1177/1087057109336586
Publikováno v:
International Journal of Science Education
This article presents an example of teacher professional development based on a perspective of situated learning and implemented on a large scale. We consider teacher professional development from three perspectives. First, teacher professional devel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f85c05196ca651cbfd9a2d37e5fbcafa
https://doi.org/10.1080/09500690802535942
https://doi.org/10.1080/09500690802535942