Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Christian M. Winterflood"'
Autor:
Oleg O. Glebov, Rachel E. Jackson, Christian M. Winterflood, Dylan M. Owen, Ellen A. Barker, Patrick Doherty, Helge Ewers, Juan Burrone
Publikováno v:
Cell Reports, Vol 18, Iss 11, Pp 2715-2728 (2017)
The active zone (AZ) matrix of presynaptic terminals coordinates the recruitment of voltage-gated calcium channels (VGCCs) and synaptic vesicles to orchestrate neurotransmitter release. However, the spatial organization of the AZ and how it controls
Externí odkaz:
https://doaj.org/article/d9660dcd63ed4bb59528a905844a1e97
Publikováno v:
Biosensors, Vol 3, Iss 1, Pp 108-115 (2013)
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming
Externí odkaz:
https://doaj.org/article/440edc153d5f4b49a4970e447f569215
Autor:
Evgenia Platonova, Helge Ewers, Jan Faix, David Albrecht, Alexander Junemann, Christian M. Winterflood
Publikováno v:
Methods, 88
With the recent development of single-molecule localization-based superresolution microscopy, the imaging of cellular structures at a resolution below the diffraction-limit of light has become a widespread technique. While single fluorescent molecule
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea2e1f579c3215320045fcbf5f451a79
Publikováno v:
Biophysical Journal. 109:3-6
Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::957a7f8eadc79f5c4424633de7c0fe0b
https://doi.org/10.1016/j.bpj.2015.05.026
https://doi.org/10.1016/j.bpj.2015.05.026
Autor:
Thomas Gronemeyer, Philip Tinnefeld, Charlotte Kaplan, Jürgen J. Schmied, Amy S. Gladfelter, Andrew A. Bridges, Sören Grinhagens, Jonas Ries, Christian M. Winterflood, Patricia Occhipinti, Bo Jing, Helge Ewers
Publikováno v:
Nano Letters
We resolved the organization of subunits in cytoskeletal polymers in cells by light microscopy. Septin GTPases form linear complexes of about 32 nm length that polymerize into filaments. We visualized both termini of septin complexes by single molecu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a82b401c6e7cb0337b79a23506a95206
https://doi.org/10.1021/acs.nanolett.5b00693
https://doi.org/10.1021/acs.nanolett.5b00693
Publikováno v:
ACS Chemical Biology. 10:1411-1416
The recent development of single-molecule localization-based super-resolution techniques has afforded a resolution in the nanometer range in light microscopy. The ability to resolve biological structures on this scale by multicolor techniques faces s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ca8e12cd170e54cead470eb61e0e1a2
https://doi.org/10.1021/acschembio.5b00046
https://doi.org/10.1021/acschembio.5b00046
Publikováno v:
Methods and Applications in Fluorescence, 3 (2)
Single particle tracking is a powerful tool to investigate the function of biological molecules by following their motion in space. However, the simultaneous tracking of two different species of molecules is still difficult to realize without comprom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::777c8aae1935fb2b8de62524c453ad83
https://pubmed.ncbi.nlm.nih.gov/29148490
https://pubmed.ncbi.nlm.nih.gov/29148490
Autor:
Cathy Fernandes, Beatriz Rico, Andre Marques-Smith, Christian M. Winterflood, Alberto Sanchez-Aguilera, Laura Mantoan, Emilia Favuzzi, Rubén Deogracias, Patricia Maeso, Helge Ewers
Publikováno v:
Favuzzi, E, Marques-Smith, A, Deogracias, R, Winterflood, C M, Sánchez-Aguilera, A, Mantoan, L, Maeso, P, Fernandes, C, Ewers, H & Rico, B 2017, ' Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican ', Neuron, vol. 95, no. 3, E10, pp. 639-655 . https://doi.org/10.1016/j.neuron.2017.06.028
Activity-dependent neuronal plasticity is a fundamental mechanism through which the nervous system adapts to sensory experience. Several lines of evidence suggest that parvalbumin (PV+) interneurons are essential in this process, but the molecular me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ae9a352d2285e5c3d507cfddd5c24a4
https://kclpure.kcl.ac.uk/en/publications/b06696a1-48e6-4e49-9da3-abc2a8fd1f7a
https://kclpure.kcl.ac.uk/en/publications/b06696a1-48e6-4e49-9da3-abc2a8fd1f7a
Autor:
Helge Ewers, Christian M. Winterflood
Publikováno v:
ChemPhysChem. 15:3447-3451
We demonstrate the potential of the commonly used red fluorescent protein mCherry for single-molecule super-resolution imaging. mCherry can be driven into a light-induced dark state in the presence of a thiol from which it can recover spontaneously o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d9df7723450f66f646322e97df0c761
https://doi.org/10.1002/cphc.201402423
https://doi.org/10.1002/cphc.201402423
Autor:
Thomas Tschager, Helge Ewers, David Albrecht, Frank Noé, Mohsen Sadeghi, Christian M. Winterflood
Publikováno v:
The Journal of Cell Biology, 215 (1)
The Journal of Cell Biology
The Journal of Cell Biology
The axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal, and transmembrane molecules. During AIS establishment, a membrane diffusion barrier is formed between the axonal and somatodendritic domains. Recently, an axonal periodic p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c84ee22c75249d7bbd13c82e8a0547df
https://hdl.handle.net/20.500.11850/121543
https://hdl.handle.net/20.500.11850/121543