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pro vyhledávání: '"Christian L. M. Vermote"'
Publikováno v:
Nucleic Acids and Molecular Biology ISBN: 9783642779527
Type II restriction/modification (R/M) systems, such as EcoRV, consist of two enzymes that act at the same DNA sequence; a modification methyltransferase and a restriction endonuclease (Bennett and Halford 1989; Wilson and Murray 1991). For EcoRV, th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ccbc2bb83a0d5df117f1ee6298f1b6e
https://doi.org/10.1007/978-3-642-77950-3_4
https://doi.org/10.1007/978-3-642-77950-3_4
Publikováno v:
Biochemistry. 31(26)
A genetic system was constructed for the mutagenesis of the EcoRV restriction endonuclease and for the overproduction of mutant proteins. The system was used to make two mutants of EcoRV, with Ala in place of either Asn185 or Asn188. In the crystal s
Publikováno v:
Biochemistry. 31(26)
In the absence of magnesium ions, the EcoRV restriction endonuclease binds all DNA sequences with equal affinity but cannot cleave DNA. In the presence of Mg2+, the EcoRV endonuclease cleaves DNA at one particular sequence, GATATC, at least a million
Publikováno v:
Biochemistry. 29(48)
The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this re