Zobrazeno 1 - 10 of 10 pro vyhledávání: '"Christian Kleusch"'
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Akademický článek
Fluorescent Lipids: Functional Parts of Fusogenic Liposomes and Tools for Cell Membrane Labeling and Visualization
Autor: Christian Kleusch, Bernd Hoffmann, Nils Hersch, Agnes Csiszár, Rudolf Merkel
Publikováno v: Molecules, Vol 17, Iss 1, Pp 1055-1073 (2012)
In this paper a rapid and highly efficient method for controlled incorporation of fluorescent lipids into living mammalian cells is introduced. Here, the fluorescent molecules have two consecutive functions: First, they trigger rapid membrane fusion
Externí odkaz: https://doaj.org/article/9b46bde116724c5a86be6d08bd17979f
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2
Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
Autor: Corinna Popp, Ralf Wagner, Maximilian G. Plach, David Peterhoff, Christian Kleusch, Philipp Schramm, Christopher Battle
Vaccines are on the front line-of-defense against infectious diseases, ranging from threats we are familiar with, including polio, tuberculosis, or HIV, to novel and emerging threats such as SARS-CoV-2. Successful development of new protein-based vac
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bb46ad387a1bbfc2df779c03bdd3c204
https://doi.org/10.1101/2020.11.30.403873
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3
Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites
Autor: Christian, Kleusch, Cornelia, Monzel, Krishna Chander, Sridhar, Bernd, Hoffmann, Agnes, Csiszár, Rudolf, Merkel
Publikováno v: International journal of molecular sciences 21(21), 8149-(2020). doi:10.3390/ijms21218149
International Journal of Molecular Sciences
Volume 21
Issue 21
International Journal of Molecular Sciences, Vol 21, Iss 8149, p 8149 (2020)
Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::67bd554fc9612a60a1a3b60b671be91a
https://juser.fz-juelich.de/record/885997
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4
Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis
Autor: Christian Kleusch, Katarzyna Walkiewicz, Dirk Daelemans, Yves A. Muller, Tanja Bartoschik, Sebastian Weigert, Stefanie Galinec, Thomas Vercruysse, Dennis Breitsprecher, Changjiang You, Nuska Tschammer, Jacob Piehler
Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
BASE-Bielefeld Academic Search Engine
Scientific Reports
MicroScale Thermophoresis (MST) is a frequently used method for the quantitative characterization of intermolecular interactions with several advantages over other technologies. One of these is its capability to determine equilibrium constants in sol
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a249c4c3637297165df419484a504b4
http://link.springer.com/article/10.1038/s41598-018-23154-3
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5
Deciphering the Functional Composition of Fusogenic Liposomes
Autor: Csiszár, Rejhana Kolašinac, Christian Kleusch, Tobias Braun, Rudolf Merkel, Agnes
Publikováno v: International Journal of Molecular Sciences; Volume 19; Issue 2; Pages: 346
Cationic liposomes are frequently used as carrier particles for nucleic acid delivery. The most popular formulation is the equimolar mixture of two components, a cationic lipid and a neutral phosphoethanolamine. Its uptake pathway has been described
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=multidiscipl::28a61400adc6150c9be5a500dcde0b1e
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6
A bioanalytical assay to distinguish cellular uptake routes for liposomes
Autor: Christian Kleusch, Rudolf Merkel, Elena Naumovska, Tobias Braun, Agnes Csiszár
Publikováno v: Cytometry Part A. 89:301-308
Lipid-based nanoparticles are frequently used for drug or DNA delivery into mammalian cells. However it is difficult to determine whether such particles are taken up via endocytosis or fusion to the plasma membrane. Here, we propose a simple and reli
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4530df7bf138f69775afe0fd74ff3052
https://doi.org/10.1002/cyto.a.22792
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7
High-throughput-feasible screening tool for determining enzyme stabilities against organic solvents directly from crude extracts
Autor: Severin Wedde, Christian Kleusch, Harald Gröger, Daniel Bakonyi
Biotransformations in organic chemistry frequently suffer from limitations caused by low water-solubility of substrates and product inhibition. Both, usually are addressed by the addition of organic co-solvents, which often accompanies at the expense
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed14620a114e0dd2d30be6d55951b716
https://pub.uni-bielefeld.de/record/2914659
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8
Resveratrol Encapsulated in Novel Fusogenic Liposomes Activates Nrf2 and Attenuates Oxidative Stress in Cerebromicrovascular Endothelial Cells From Aged Rats
Autor: Christian Kleusch, Agnes Csiszár, John T. Pinto, Bernd Hoffmann, Zoltan Ungvari, Tripti Gautam, Zsuzsanna Tucsek, Peter Toth, William E. Sonntag, Anna Csiszar
Publikováno v: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 70:303-313
Resveratrol (3,4',5-trihydroxystilbene) is a plant-derived polyphenolic trans-stilbenoid, which exerts multifaceted antiaging effects. Here, we propose a novel delivery system for resveratrol, which significantly increases its cellular uptake into ag
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cccef24aa853d0a475304c4ab1bcb2f
https://doi.org/10.1093/gerona/glu029
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9
A bioanalytical assay to distinguish cellular uptake routes for liposomes
Autor: Tobias, Braun, Christian, Kleusch, Elena, Naumovska, Rudolf, Merkel, Agnes, Csiszár
Publikováno v: Cytometry. Part A : the journal of the International Society for Analytical Cytology. 89(3)
Lipid-based nanoparticles are frequently used for drug or DNA delivery into mammalian cells. However it is difficult to determine whether such particles are taken up via endocytosis or fusion to the plasma membrane. Here, we propose a simple and reli
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d5e1ec3eb7918919a9e75dfb0db47d41
https://pubmed.ncbi.nlm.nih.gov/26551759
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10
Fluorescent lipids: functional parts of fusogenic liposomes and tools for cell membrane labeling and visualization
Autor: Rudolf Merkel, Agnes Csiszár, Nils Hersch, Bernd Hoffmann, Christian Kleusch
Publikováno v: Molecules 17, 1055-1073 (2012). doi:10.3390/molecules17011055
Molecules
Molecules, Vol 17, Iss 1, Pp 1055-1073 (2012)
Molecules; Volume 17; Issue 1; Pages: 1055-1073
In this paper a rapid and highly efficient method for controlled incorporation of fluorescent lipids into living mammalian cells is introduced. Here, the fluorescent molecules have two consecutive functions: First, they trigger rapid membrane fusion
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90e328dd358a079962a7a1851d78c54d
https://hdl.handle.net/2128/11532
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