Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Christian J. Koehler"'
Publikováno v:
Molecules, Vol 26, Iss 17, p 5330 (2021)
(1) Background: Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter ion-based isobaric labeling methods
Externí odkaz:
https://doaj.org/article/c25af50cca964f88993013d9abdde290
Publikováno v:
Separations, Vol 8, Iss 5, p 66 (2021)
This paper describes smart sampling paper to be used for bottom-up protein analysis. Four different manners to immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized paper (with and without post-immobiliza
Externí odkaz:
https://doaj.org/article/05d0203e849741bdb401f01a8a97ef5e
Autor:
Christian J. Koehler, Bernd Thiede
Publikováno v:
Journal of Biological Inorganic Chemistry
Proteolytic digestion prior to LC–MS analysis is a key step for the identification of proteins. Digestion of proteins is typically performed with trypsin, but certain proteins or important protein sequence regions might be missed using this endopro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f833d92ee5d1f8d389acb449b51840a2
https://doi.org/10.1007/s00775-019-01733-7
https://doi.org/10.1007/s00775-019-01733-7
Autor:
Christian J, Koehler, Bernd, Thiede
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2228
Isobaric peptide termini labeling (IPTL) is an approach for quantitative proteomics based on crosswise isotopic labeling of peptides at the N- and C-terminus. The labeling reagents are chosen in isotopic variations that the resulting mass of all labe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7b0aaa0c33804320ca0a91c5bd80bb24
https://pubmed.ncbi.nlm.nih.gov/33950488
https://pubmed.ncbi.nlm.nih.gov/33950488
Publikováno v:
Separations; Volume 8; Issue 5; Pages: 66
Separations, Vol 8, Iss 66, p 66 (2021)
Separations, Vol 8, Iss 66, p 66 (2021)
This paper describes smart sampling paper to be used for bottom-up protein analysis. Four different manners to immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized paper (with and without post-immobiliza
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68d32f5ede270cbdae98d580dbfd0c61
http://hdl.handle.net/10852/89981
http://hdl.handle.net/10852/89981
Autor:
Bernd Thiede, Christian J. Koehler
Publikováno v:
Methods in Molecular Biology ISBN: 9781071610237
Isobaric peptide termini labeling (IPTL) is an approach for quantitative proteomics based on crosswise isotopic labeling of peptides at the N- and C-terminus. The labeling reagents are chosen in isotopic variations that the resulting mass of all labe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ae2c3b01ea9d160263f8051a68ddf8a
https://doi.org/10.1007/978-1-0716-1024-4_10
https://doi.org/10.1007/978-1-0716-1024-4_10
Publikováno v:
Molecules
Molecules, Vol 26, Iss 5330, p 5330 (2021)
Volume 26
Issue 17
Molecules, Vol 26, Iss 5330, p 5330 (2021)
Volume 26
Issue 17
(1) Background: Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter ion-based isobaric labeling methods
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb7f9b1a6e92d2b6fd42f0b2d54290b3
http://hdl.handle.net/10852/90254
http://hdl.handle.net/10852/90254
Autor:
Bernd Thiede, Ravi Chand Bollineni, Christian J. Koehler, Randi Elin Gislefoss, Jan Haug Anonsen
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
Scientific Reports
Scientific Reports
Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics. We present an approach for automated annotation of intact glycopeptide mass spectra. The steps in adopting the Mascot search engine for intact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8bfd320c906d47476e31b348fb7a455
http://link.springer.com/article/10.1038/s41598-018-20331-2
http://link.springer.com/article/10.1038/s41598-018-20331-2
Autor:
Siri Dørum, Magnus Ø Arntzen, Shuo-Wang Qiao, Anders Holm, Christian J Koehler, Bernd Thiede, Ludvig M Sollid, Burkhard Fleckenstein
Publikováno v:
PLoS ONE, Vol 5, Iss 11, p e14056 (2010)
BACKGROUND: Celiac disease is a T-cell mediated chronic inflammatory disorder of the gut that is induced by dietary exposure to gluten proteins. CD4+ T cells of the intestinal lesion recognize gluten peptides in the context of HLA-DQ2.5 or HLA-DQ8 an
Externí odkaz:
https://doaj.org/article/2dca723513514852b58ae10362dd2d2f
Autor:
Trung T. Tran, Christian J. Koehler, Bernd Thiede, Margarita Strozynski, Ravi Chand Bollineni
Publikováno v:
Journal of Proteome Research. 16:2571-2578
Alternative splicing is a mechanism in eukaryotes by which different forms of mRNAs are generated from the same gene. Identification of alternative splice variants requires the identification of peptides specific for alternative splice forms. For thi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef7725ebd55a359269a6181b2a2f2520
https://doi.org/10.1021/acs.jproteome.7b00126
https://doi.org/10.1021/acs.jproteome.7b00126