Assessment of the percentage of full recombinant adeno-associated virus particles in a gene therapy drug using CryoTEM

Autor: Mathieu Colomb-Delsuc, Roman Raim, Christian Fiedler, Stefan Reuberger, Johannes Lengler, Rickard Nordström, Martin Ryner, Ioana Mihaela Folea, Barbara Kraus, Juan A. Hernandez Bort, Ida-Maria Sintorn

Publikováno v: PLoS ONE, Vol 17, Iss 6 (2022)

In spite of continuous development of gene therapy vectors with thousands of drug candidates in clinical drug trials there are only a small number approved on the market today stressing the need to have characterization methods to assist in the valid

Externí odkaz: https://doaj.org/article/ef333d3a4b1c483fa17c63bba0d3cba0

The Role of PAR2 in TGF-β1-Induced ERK Activation and Cell Motility

Autor: Hendrik Ungefroren, David Witte, Christian Fiedler, Thomas Gädeken, Roland Kaufmann, Hendrik Lehnert, Frank Gieseler, Bernhard H. Rauch

Publikováno v: International Journal of Molecular Sciences, Vol 18, Iss 12, p 2776 (2017)

Background: Recently, the expression of proteinase-activated receptor 2 (PAR2) has been shown to be essential for activin receptor-like kinase 5 (ALK5)/SMAD-mediated signaling and cell migration by transforming growth factor (TGF)-β1. However, it is

Externí odkaz: https://doaj.org/article/58d652909d714758b3e1c23dabc7e272

Identification of cysteine thiol‐based linkages in ADAMTS13 in support of a non‐proteolytic regulation of von Willebrand factor

Autor: Birgit K. Seyfried, Friedrich Scheiflinger, Barbara Plaimauer, X. Long Zheng, Christian Fiedler, Stefan Kaufmann, Hanspeter Rottensteiner, Jing-fei Dong

Publikováno v: Journal of Thrombosis and Haemostasis

Background ADAMTS13, a plasma metalloprotease, cleaves von Willebrand factor (VWF) to regulate its function. Additionally, ADAMTS13 is thought to regulate lateral association of VWF multimers to form fibrillar structures through its free thiols. Obje

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d223ddd6eb626937e35558731fd8938
http://europepmc.org/articles/PMC6916347

Practical and Rigorous Uncertainty Bounds for Gaussian Process Regression

Autor: Christian Fiedler, Carsten W. Scherer, Sebastian Trimpe

Publikováno v: The Thirty-Fifth AAAI Conference on Artificial Intelligence, the Thirty-Third Conference on Innovative Applications of Artificial Intelligence, the Eleventh Symposium on Educational Advances in Artificial Intelligence

Gaussian Process Regression is a popular nonparametric regression method based on Bayesian principles that provides uncertainty estimates for its predictions. However, these estimates are of a Bayesian nature, whereas for some important applications,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de04f087927655879ff1ffa593e4777c
http://arxiv.org/abs/2105.02796

RAC1B Induces SMAD7 via USP26 to Suppress TGFβ1-Dependent Cell Migration in Mesenchymal-Subtype Carcinoma Cells

Autor: Hendrik Ungefroren, Melina Musfeldt, Jens-Uwe Marquardt, Christian Fiedler, Hendrik Lehnert, Anuradha Kumarasinghe

Publikováno v: Cancers
Volume 12
Issue 6
Cancers, Vol 12, Iss 1545, p 1545 (2020)

The small GTPase RAC1B has been shown to act as a powerful inhibitor of the transforming growth factor (TGF)&beta
type I receptor ALK5 and TGF&beta
1/ALK5-induced epithelial&ndash
mesenchymal transition and cell motility. However, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93ae2fd92dc44292d69547f91019c8f2