Výsledky vyhledávání - "Christi L. Clogston"

Structure of the active core of human stem cell factor and analysis of binding to its receptor Kit

Autor: Ogan Gurel, Rashid Syed, Wayne A. Hendrickson, Hsieng Sen Lu, Xuliang Jiang, Elizabeth A. Mendiaz, Osslund Timothy D, Keith Langley, Christi L. Clogston, George Stearns

Publikováno v: The EMBO Journal. 19:3192-3203

Stem cell factor (SCF) is an early-acting hematopoietic cytokine that elicits multiple biological effects. SCF is dimeric and occurs in soluble and membrane-bound forms. It transduces signals by ligand- mediated dimerization of its receptor, Kit, whi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::231d8b3808d4c43332de25374952404c
https://doi.org/10.1093/emboj/19.13.3192

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Purification and biologic characterization of plasma-derived megakaryocyte growth and development factor

Autor: Susan Swift, Hsieng Sen Lu, Jim Skrine, P Hunt, Christi L. Clogston, Janet L. Nichol, Martha M. Hokom, Yanli Li, Jakob M. Bogenberger, A Hornkohl

Publikováno v: Blood. 86:540-547

The isolation and cloning of the ligand for the cytokine receptor, Mpl, have been recently described. In this report we present details of the purification of this novel cytokine (megakaryocyte growth and development factor [MGDF]) from aplastic cani

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b8fd8b5a9c3a966e66a7ed020ce6415d
https://doi.org/10.1182/blood.v86.2.540.bloodjournal862540

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Soluble kit receptor in human serum

Autor: Meredith G. Schwartz, Christi L. Clogston, Vann P. Parker, Larry Bennett, Jette Wypych, Hsieng Sen Lu, Keith Langley, Timothy D. Bartley, Virginia C. Broudy

Publikováno v: Blood. 85:66-73

c-kit encodes the transmembrane receptor tyrosine kinase (Kit) for the recently described ligand stem cell factor (SCF). We have developed an enzyme-linked immunosorbent assay for measuring soluble human Kit and we have used the assay to show high le

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6e9bedd261a7607beb3f47c3a9bf2da8
https://doi.org/10.1182/blood.v85.1.66.bloodjournal85166

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Peptide Map Analysis of Recombinant Human Granulocyte Colony Stimulating Factor: Elimination of Methionine Modification and Nonspecific Cleavages

Autor: Christi L. Clogston, Hsieng Sen Lu, Lee Anne Merewether, Michael D. Jones

Publikováno v: Analytical Biochemistry. 216:135-146

Procedures for HPLC peptide map analysis of recombinant human granulocyte colony stimulating factor include reduction and S -carboxymethylation of the denatured protein, as well as protease digestion with Staphyloccus aureus endoproteinase Glu-C foll

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5659594470f83ca800690befa72e937
https://doi.org/10.1006/abio.1994.1017

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Isolation and Characterization of Three Recombinant Human Granulocyte Colony Stimulating Factor His → Gln Isoforms Produced in Escherichia coli

Autor: A.C. Herman, Christi L. Clogston, Hsieng Sen Lu, L.S. Sotos, P.R. Fausset, K.S. Stoney, Michael F. Rohde

Publikováno v: Protein Expression and Purification. 4:465-472

This report demonstrates that three variant isoforms of recombinant methionyl human granulocyte colony stimulating factor are present in small quantities in the crude preparation solubilized from Escherichia coli inclusion bodies. These isoforms were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28b9e164bd5c4fe3df898147c8498857
https://doi.org/10.1006/prep.1993.1061

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Post-translational processing of membrane-associated recombinant human stem cell factor expressed in Chinese hamster ovary cells

Autor: Hsieng Sen Lu, Subhash B. Karkare, Sylvia Hu, Xiao-Dong Liu, Krisztina M. Zsebo, Steven G. Ogden, Kristina Swiderek, Christi L. Clogston, Vann P. Parker, Terry D. Lee, David C. Chang, Keith Langley, Avantika C. Patel, David W. Brankow, Robert F. Baltera, Jette Wypych

Publikováno v: Archives of Biochemistry and Biophysics. 298:150-158

This report describes the structure of soluble human stem cell factor isolated from the conditioned medium of Chinese hamster ovary (CHO) cells transfected with stem cell factor (SCF) cDNA, which encodes a leader sequence plus 248 additional amino ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8d103a777a2bcae34133e2f02a7d7ba
https://doi.org/10.1016/0003-9861(92)90106-7

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Detection and quantitation of recombinant granulocyte colony-stimulating factor charge isoforms: Comparative analysis by cationic-exchange chromatography, isoelectric focusing gel electrophoresis, and peptide mapping

Autor: Thomas C. Boone, Hsieng Sen Lu, Yueh-Rong Hsu, Christi L. Clogston

Publikováno v: Analytical Biochemistry. 202:375-383

Routine quantitation of recombinant human granulocyte colony-stimulating factor charge isoforms in the purified protein product requires development of a reliable analytical method. In this report, isoelectric focusing gel electrophoresis, peptide ma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::508e38402391089659b72c0088d04e94
https://doi.org/10.1016/0003-2697(92)90121-m

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Folding and oxidation of recombinant human granulocyte colony stimulating factor produced in Escherichia coli. Characterization of the disulfide-reduced intermediates and cysteine—-serine analogs

Autor: Linda O. Narhi, L A Merewether, Christi L. Clogston, W R Pearl, Thomas C. Boone, Hsieng Sen Lu

Publikováno v: Journal of Biological Chemistry. 267:8770-8777

The folding and oxidation of recombinant human granulocyte colony-stimulating factor solubilized from Escherichia coli inclusion bodies was investigated. During the folding process, two intermediates, I1 and I2, were detected by kinetic studies using

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::de92ba3bb78a8d1f0ea4dbac5459e4e3
https://doi.org/10.1016/s0021-9258(19)50345-3

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Glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure

Autor: J.W. Lary, Krisztina M. Zsebo, D.A. Yphantis, Linda O. Narhi, S J Prestrelski, Christi L. Clogston, Hsieng Sen Lu, Jette Wypych, Tsutomu Arakawa, Elizabeth A. Mendiaz

Publikováno v: Journal of Biological Chemistry. 266:18942-18948

We have recently described the identification, isolation, and characterization of a factor, termed stem cell factor (SCF), which acts on primitive hematopoietic progenitors of the marrow. A soluble form of the factor was isolated from the conditioned

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0ef9a4d22c70ed4da33ab8042e9bbb03
https://doi.org/10.1016/s0021-9258(18)55154-1

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