Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Christel Hempen"'
Publikováno v:
Analytica Chimica Acta. 560:35-40
A new liquid chromatography/atmospheric pressure chemical ionization-tandem mass spectrometry (LC/APCI-MS/MS) method with on-line sample clean-up for the determination of telmisartan in human blood plasma is presented. This technique is compared to a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95468e9d88b45cdaa2fba84e62a7563f
https://doi.org/10.1016/j.aca.2005.12.036
https://doi.org/10.1016/j.aca.2005.12.036
Autor:
Christel Hempen, Uwe Karst
Publikováno v:
Analytical and bioanalytical chemistry, 384(3), 572-583. Springer
Different labeling strategies for enzymatic assays and immunoassays are reviewed. Techniques which make use of direct detection of a label, e.g. radioimmunoassays, are discussed, as are techniques in which the label is associated with inherent signal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75f22550b5470a40b009250d87f177da
https://doi.org/10.1007/s00216-005-3392-0
https://doi.org/10.1007/s00216-005-3392-0
Publikováno v:
Analytical and Bioanalytical Chemistry. 382:234-238
Mass spectrometric evidence was obtained to confirm that the main reaction product of the horseradish peroxidase (POD)-catalyzed oxidation of o-phenylenediamine (OPD) by hydrogen peroxide is 2,3-diaminophenazine. Although this reaction is one of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bdf6d47d11d32843d4a84be0aabe9e7
https://doi.org/10.1007/s00216-005-3178-4
https://doi.org/10.1007/s00216-005-3178-4
Publikováno v:
Analytica chimica acta, 543(1-2), 137-142. Elsevier
Fluorescence and mass spectrometric detection schemes are developed and compared for the simultaneous activity determination of two enzymes in solution. As model system, the following reactions are used: The alkaline phosphatase catalyzed reaction wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9c10a822426020a53de7d7d3fd5a031
https://doi.org/10.1016/j.aca.2005.04.038
https://doi.org/10.1016/j.aca.2005.04.038
Autor:
Christel Hempen, Uwe Karst
Publikováno v:
Analytica chimica acta, 521(2), 117-122. Elsevier
A detection scheme for the simultaneous evaluation of two bioassays based on fluorescence spectroscopy is presented. For the determination of hydrogen peroxide-generating enzymes or peroxidases, the non-fluorescent 4-(N-methylhydrazino)-7-nitro-2,1,3
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d7e8db09fb3d230c580e85a653e246f
https://doi.org/10.1016/j.aca.2004.06.007
https://doi.org/10.1016/j.aca.2004.06.007
Publikováno v:
Journal of chromatography. B: Analytical technologies in the biomedical and life sciences, 830(1), 47-53. Elsevier
Journal of Chromatography B, 830(1), 47-53. Elsevier
Haselberg, R, Hempen, C M, van Leeuwen, SM, Vogel, M & Karst, U 2006, ' Analysis of microperoxidases using liquid chromatography, post-column substrate conversion and fluorescence detection ', Journal of Chromatography B, vol. 830, no. 1, pp. 47-53 . https://doi.org/10.1016/j.jchromb.2005.10.013
Journal of Chromatography B, 830(1), 47-53. Elsevier
Haselberg, R, Hempen, C M, van Leeuwen, SM, Vogel, M & Karst, U 2006, ' Analysis of microperoxidases using liquid chromatography, post-column substrate conversion and fluorescence detection ', Journal of Chromatography B, vol. 830, no. 1, pp. 47-53 . https://doi.org/10.1016/j.jchromb.2005.10.013
A liquid chromatographic method with on-line activity determination for microperoxidases has been developed. After enzymatic digestion of a cytochrome, possibly under formation of microperoxidases, the product mixture is separated by reversed-phase l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67526ce50cf1152e23c824cbbf5cd70b
https://pubmed.ncbi.nlm.nih.gov/16257275
https://pubmed.ncbi.nlm.nih.gov/16257275