Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Chris De Wilde"'
Publikováno v:
Molecular Plant-Microbe Interactions, Vol 13, Iss 6, Pp 658-665 (2000)
Using the Cre/lox recombination system, we analyzed the extent to which T-DNA transfer to the plant cell and T-DNA integration into the plant genome determine the transformation and cotransformation frequencies of Arabidopsis root cells. Without sele
Externí odkaz:
https://doaj.org/article/a7916279fa424c14bc3362592c7f53c8
Autor:
Ingrid Peck, Anna Depicker, Gordana Marjanac, Annelies De Paepe, Jonah Nolf, Sylvie De Buck, Chris De Wilde
Publikováno v:
PLANT PHYSIOLOGY
We investigated whether complex T-DNA loci, often resulting in low transgene expression, can be resolved efficiently into single copies by CRE/loxP-mediated recombination. An SB-loxP T-DNA, containing two invertedly oriented loxP sequences located in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0287a59d0cce3e116e1f9367cddef0fe
https://doi.org/10.1104/pp.107.104067
https://doi.org/10.1104/pp.107.104067
Autor:
Katrien Lievens, Pierre Lejeune, Christophe Reuzeau, Wim van Camp, Valerie Frankard, Joris de Wolf, Ernst Vranken, Rindert Peerbolte, Anabel Sanz, Yves Hatzfeld, Willem F. Broekaert, Chris De Wilde
Publikováno v:
Plant Genetic Resources. 4:20-24
The improvement of quality and quantitative traits in industrial crops is among the most important goals in plant breeding. Many traits of interest are controlled by multiple genes and improvements have so far only been obtained through conventional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::92fdfde9b1cbe5b85ef2ba628978f7c1
https://doi.org/10.1079/pgr2005104
https://doi.org/10.1079/pgr2005104
Publikováno v:
Molecular Breeding. 9:271-282
To use transgenic potato tubers (Solanum tuberosum cv. Desiree) for bulk production of recombinant antibodies, constructs were engineered for accumulating full-size IgGs and Fab fragments in the plant cell apoplast or endoplasmic reticulum (ER). An i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91f693b9518edc89b43a02f220ffbc82
https://doi.org/10.1023/a:1020306917914
https://doi.org/10.1023/a:1020306917914
Publikováno v:
European Journal of Biochemistry. 268:4251-4260
To further improve antibody production in plants, constructs were designed to minimize transgene silencing and to retain a F(ab) fragment within the secretory pathway of transgenic Arabidopsis thaliana plants. The levels of antibody accumulation sugg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::923f6d38b0942fad50ae3506ac5e2603
https://doi.org/10.1046/j.1432-1327.2001.02340.x
https://doi.org/10.1046/j.1432-1327.2001.02340.x
Publikováno v:
Plant Molecular Biology. 43:419-428
Immunomodulation is a molecular technique that allows the interference with cellular metabolism or pathogen infectivity by the ectopic expression of genes encoding antibodies or antibody fragments. In recent years, several reports have proven the val
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2f3c8634893b51d7771d154756a7421
https://doi.org/10.1023/a:1006471528756
https://doi.org/10.1023/a:1006471528756
Autor:
Geert De Jaeger, Anna Depicker, Sylvie De Buck, Helena Van Houdt, Chris De Wilde, Geert Angenon
Publikováno v:
Plant Molecular Biology. 43:347-359
Plants are particularly attractive as large-scale production systems for proteins intended for therapeutical or industrial applications: they can be grown easily and inexpensively in large quantities that can be harvested and processed with the avail
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::621eddd0ff09cc80e91128234c8ca9cd
https://doi.org/10.1023/a:1006464304199
https://doi.org/10.1023/a:1006464304199
Publikováno v:
Molecular Breeding. 6:459-468
Transgenic Arabidopsis and tobacco plants (125) derived from seven Agrobacterium-mediated transformation experiments were screened by polymerase chain reaction and DNA gel blot analysis for the presence of vector `backbone' sequences. The percentage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::44c7c94408a07482a02d18a1621f1838
https://doi.org/10.1023/a:1026575524345
https://doi.org/10.1023/a:1026575524345
Autor:
Chris De Wilde, Geert Angenon, Tom Gerats, Anna Depicker, Geert De Jaeger, Emmanuel S. Buys, Anni Jacobs, Marc Van Montagu, Dominique Eeckhout, Jyoti Kapila
Publikováno v:
European Journal of Biochemistry. 259:426-434
The accumulation of five murine single-chain variable fragments, binding to dihydroflavonol 4-reductase, was analyzed in transgenic Petunia hybrida plants. The five scFv-encoding sequences were cloned in an optimized plant transformation vector for e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb8a9a32d233b46e30be999ac8fd743d
https://doi.org/10.1046/j.1432-1327.1999.00060.x
https://doi.org/10.1046/j.1432-1327.1999.00060.x
Autor:
Geert De Jaeger, Anne-Marie Bruyns, Anna Depicker, Myriam De Neve, Chris De Wilde, Gilbert Engler, Riet De Rycke, Marc Van Montagu
Publikováno v:
Plant Science. 114:233-241
The in planta localization of a full-size immunoglobulin G (IgG) antibody and of its derived F ab fragment were determined using immunogold electron microscopy on transgenic Arabidopsis thaliana plants. Although it is widely assumed that antibodies,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eb2c4e406a84ddc6113b6b2948df51ac
https://doi.org/10.1016/0168-9452(96)04331-2
https://doi.org/10.1016/0168-9452(96)04331-2