Zobrazeno 1 - 10
of 416
pro vyhledávání: '"Chris D Geddes"'
Autor:
Tonya M Santaus, Shan Li, Lahari Saha, Wilbur H Chen, Siya Bhagat, O Colin Stine, Chris D Geddes
Publikováno v:
PLoS ONE, Vol 14, Iss 7, p e0220102 (2019)
The ability for safe and rapid pathogenic sample transportation and subsequent detection is an increasing challenge throughout the world. Herein, we describe and use bead-beating, vortex, sonication, 903 protein saver cards, and Lyse-It methods, aimi
Externí odkaz:
https://doaj.org/article/2bafc6d4f6874f41af329aa36138b88d
Publikováno v:
PLoS ONE, Vol 14, Iss 12, p e0225475 (2019)
Rapid sample preparation is one of the leading bottlenecks to low-cost and efficient sample component detection. To overcome this setback, a technology known as Lyse-It has been developed to rapidly (less than 60 seconds) lyse Gram-positive and-negat
Externí odkaz:
https://doaj.org/article/2475d06aea8d4ff281fd7f2a8fc2ca09
Publikováno v:
PLoS ONE, Vol 14, Iss 9, p e0223008 (2019)
Nucleases are enzymes that can degrade genomic DNA and RNA that decrease the accuracy of quantitative measures of those nucleic acids. Here, we study conventional heating, standard microwave irradiation, and Lyse-It, a microwave-based lysing technolo
Externí odkaz:
https://doaj.org/article/9fd3b8e0dd2d4bc987b8409078f861f2
Autor:
Tonya M Santaus, Shan Li, Paula Ladd, Amanda Harvey, Shannon Cole, O Colin Stine, Chris D Geddes
Publikováno v:
PLoS ONE, Vol 13, Iss 7, p e0201070 (2018)
Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio chole
Externí odkaz:
https://doaj.org/article/159999236d7641b8b73b3532a3fd1760
Publikováno v:
ACS Omega, Vol 9, Iss 23, Pp 25181-25188 (2024)
Externí odkaz:
https://doaj.org/article/b759438b9077442d893781defada3c70
Publikováno v:
PLoS ONE, Vol 9, Iss 8, p e104334 (2014)
Clostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key ste
Externí odkaz:
https://doaj.org/article/073365667e444e4fa158cf6d7e04f31f
Autor:
Chiguang Feng, Nicholas M Stamatos, Anatoliy I Dragan, Andrei Medvedev, Melissa Whitford, Lei Zhang, Chang Song, Prasad Rallabhandi, Leah Cole, Quan M Nhu, Stefanie N Vogel, Chris D Geddes, Alan S Cross
Publikováno v:
PLoS ONE, Vol 7, Iss 4, p e32359 (2012)
We previously reported that neuraminidase (NA) pretreatment of human PBMCs markedly increased their cytokine response to lipopolysaccharide (LPS). To study the mechanisms by which this occurs, we transfected HEK293T cells with plasmids encoding TLR4,
Externí odkaz:
https://doaj.org/article/d1a75ee7f6f44000aa3d9aa8b1ba3a10
Publikováno v:
PLoS ONE, Vol 6, Iss 4, p e18700 (2011)
Certain serovars of Salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. A rapid, sensitive diagnostic test is needed to al
Externí odkaz:
https://doaj.org/article/4f1609f86a3e4d069f9349484aaf82bc
Publikováno v:
The Journal of Physical Chemistry C. 127:1126-1134
Publikováno v:
Plasmonics. 16:1765-1772
The deleterious effects of reactive oxygen species (ROS), including singlet oxygen (1O2), on biological systems have cultivated widespread interest in fields ranging from therapeutic techniques to sterilization materials. Researchers have, for exampl