Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Chris Burbage"'
Publikováno v:
Marine pollution bulletin. 149
Microplastics in wastewater treatment plant (WWTP) effluent have been identified and quantified, but few studies have examined the microplastics in advanced treatment systems. A new method for isolating, quantifying, and determining the polymer type
Autor:
Kshitiz Uprety, Chris Burbage, Bill Balzer, Katya Bilyk, Ron Latimer, Charles Bott, Kevin Parker
Publikováno v:
Proceedings of the Water Environment Federation. 2012:3747-3771
Autor:
Arthur E. Frankel, Philip D. Hall, Robert J. Kreitman, Kapil N. Bhalla, Joseph Vesely, Mark C. Willingham, Chris Burbage
Publikováno v:
Blood. 90:3654-3661
It has previously been shown that human granulocyte-macrophage colony-stimulating factor (GM-CSF) can be fused to a truncated diphtheria toxin (DT) to produce a recombinant fusion toxin that kills GM-CSF receptor–bearing cells. We now report that D
Publikováno v:
Leukemia. 11:22-30
Interleukin-2 (IL2) fused to ricin B chain (RTB) with modifications of amino acid residues in each of three galactose-binding subdomains (1alpha, 1beta and 2gamma) was expressed in insect cells, purified by immunoaffinity chromatography and reassocia
Autor:
Arthur E. Frankel, Edward P. Tagge, Greg Hancock, Mark C. Willingham, John Chandler, Joseph Vesely, Chris Burbage
Publikováno v:
Bioconjugate Chemistry. 7:30-37
DNA encoding ricin B chain was modified by site-directed mutagenesis, and eight separate mutant RTB cDNAs including four novel mutants were ligated into the baculovirus transfer vector, pAcGP67A. Cotransfection of S. frugiperda Sf9 cells with BaculoG
Publikováno v:
Biochemistry. 35:14749-14756
Ricin toxin, the heterodimeric 65 kDa glycoprotein synthesized in castor bean seeds, consists of a cell binding lectin subunit (RTB) disulfide linked to an rRNA N-glycosidase protein synthesis inactivating subunit (RTA). While X-ray crystallography a
Publikováno v:
"Protein Engineering, Design and Selection". 9:913-919
Fusion toxins are hybrid proteins consisting of peptide ligands linked through amide bonds to polypeptide toxins. The ligand directs the molecule to the surface of target cells and the toxin enters the cytosol and induces cell death. Ricin is an exce
Autor:
Mark C. Willingham, Greg Hancock, Joseph Vesely, Chris Burbage, Arthur E. Frankel, Edward P. Tagge, John Chandler
Publikováno v:
Bioconjugate Chemistry. 6:666-672
Fusion toxins consist of peptide ligands linked through amide bonds to polypeptide toxins. The ligand directs the molecule to the surface of target cells and the toxin enters the cytosol and induces cell death. Ricin toxin is an excellent candidate f
Autor:
Philip D. Hall, Arthur E. Frankel, Edward P. Tagge, Joseph Vesely, Chris Burbage, Billie Harris, Mark C. Willingham
Publikováno v:
Bioconjugate chemistry. 8(5)
We performed genetic engineering to fuse enhanced green fluorescent protein (EGFP) to the N terminus of RTA, expressed the fusion protein in Escherichia coli, purified and reassociated EGFP-RTA with plant RTB, and purified EGFP-ricin by size exclusio
Autor:
Tao Fu, Chris Burbage, Edward P. Tagge, Thomas Brothers, Mark C. Willingham, Arthur E. Frankel
Publikováno v:
International journal of immunopharmacology. 18(12)
Ricin intoxication of mammalian cells is initiated by B chain (RTB) binding to cell surface galactosides. Recombinant insect-derived RTB mutants with modifications in lectin-site subdomains 2 gamma, 1 alpha/2 gamma, and 1 alpha/1 beta/2 gamma were re