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pro vyhledávání: '"Choll Wan Kim"'
Publikováno v:
Journal of Molecular Biology. 231:960-981
Random sequencing of cDNA and genomic libraries has been used to study the genome of the hyperthermophile Thermotoga maritima. To date, 175 unique clones have been analyzed by comparing short sequence tags with known proteins in the PIR and GenBank d
Publikováno v:
Proteins: Structure, Function, and Genetics. 13:352-363
Amino acid substitution analysis within a highly conserved region of Escherichia coli thymidylate synthase (TS), using suppression of amber mutations by tRNA suppressors, has yielded a bank of 124 new mutationally altered TS proteins. These mutant pr
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 87(10)
By using site-directed oligonucleotide mutagenesis, amber nonsense stop codons (5'-TAG-3') have been introduced at 20 sites in the Escherichia coli thymidylate synthase gene. By transforming the thyA mutant plasmids into 13 strains, each of which har