Author Correction: High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2.

Autor: Chan CWF; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Shatin, Hong Kong SAR, China., Wang B; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Shatin, Hong Kong SAR, China., Nan L; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Advanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, Hong Kong SAR, China., Huang X; State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Mao T; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Advanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, Hong Kong SAR, China., Chu HY; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Shatin, Hong Kong SAR, China., Luo C; State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chu H; State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. hinchu@hku.hk.; Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science Park, Shatin, Hong Kong SAR, China. hinchu@hku.hk.; Department of Infectious Disease and Microbiology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China. hinchu@hku.hk., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. gigichoi@hku.hk.; Centre for Oncology and Immunology, Hong Kong Science Park, Shatin, Hong Kong SAR, China. gigichoi@hku.hk.; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. gigichoi@hku.hk., Shum HC; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. ashum@hku.hk.; Advanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, Hong Kong SAR, China. ashum@hku.hk., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. aslw@hku.hk.; Centre for Oncology and Immunology, Hong Kong Science Park, Shatin, Hong Kong SAR, China. aslw@hku.hk.

Publikováno v: Nature biomedical engineering [Nat Biomed Eng] 2024 Mar; Vol. 8 (3), pp. 336.

A synthetic transcription platform for programmable gene expression in mammalian cells.

Autor: Chen WCW; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. William.Chen@usd.edu.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. William.Chen@usd.edu.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. William.Chen@usd.edu.; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, 02114, USA. William.Chen@usd.edu.; Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USA. William.Chen@usd.edu., Gaidukov L; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Lai Y; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Wu MR; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, 02215, USA., Cao J; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Gutbrod MJ; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA, 02139, USA., Choi GCG; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China., Utomo RP; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Biochemistry, Wellesley College, Wellesley, MA, 02481, USA., Chen YC; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan., Wroblewska L; Pfizer Inc., Cambridge, MA, 02139, USA., Kellis M; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA, 02139, USA., Zhang L; Pfizer Inc., Andover, MA, 01810, USA., Weiss R; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Lu TK; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. tim@lugroup.org.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. tim@lugroup.org.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. tim@lugroup.org.

Publikováno v: Nature communications [Nat Commun] 2022 Oct 18; Vol. 13 (1), pp. 6167. Date of Electronic Publication: 2022 Oct 18.

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination.

Autor: Yuen CTL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Thean DGL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chan BKC; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China., Zhou P; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Kwok CCS; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chu HY; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China., Cheung MSH; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Wang B; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Chan YM; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China., Mak SYL; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China., Leung AY; Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China.; Division of Haematology, Department of Medicine, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; The Jockey Club Centre for Clinical Innovation and Discovery, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China., Zheng Z; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong SAR, China.; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China.; Biotechnology and Health Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2022 Feb 22; Vol. 50 (3), pp. 1650-1660.

High-throughput 5' UTR engineering for enhanced protein production in non-viral gene therapies.

Autor: Cao J; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Novoa EM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Genomic Regulation (CRG), Barcelona, Spain., Zhang Z; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA., Chen WCW; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA., Liu D; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA., Choi GCG; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.; School of Biomedical Sciences, University of Hong Kong, Hong Kong, China., Wong ASL; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.; School of Biomedical Sciences, University of Hong Kong, Hong Kong, China., Wehrspaun C; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA., Kellis M; Broad Institute of MIT and Harvard, Cambridge, MA, USA. manoli@mit.edu.; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA. manoli@mit.edu.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA. manoli@mit.edu., Lu TK; Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA. timlu@mit.edu.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. timlu@mit.edu.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA. timlu@mit.edu.; Broad Institute of MIT and Harvard, Cambridge, MA, USA. timlu@mit.edu.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA. timlu@mit.edu.

Publikováno v: Nature communications [Nat Commun] 2021 Jul 06; Vol. 12 (1), pp. 4138. Date of Electronic Publication: 2021 Jul 06.

High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis.

Autor: Wan YK; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China., Choi GCG; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China., Wong ASL; Laboratory of Combinatorial Genetics and Synthetic Biology, School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China. aslw@hku.hk.; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong SAR, China. aslw@hku.hk.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2199, pp. 3-12.