5β-reduced neuroactive steroids as modulators of growth and viability of postnatal neurons and glia.

Autor: Munawar Cheema M; Laboratory of Molecular Neurobiology, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague 4, Czech Republic., Macakova Kotrbova Z; CZ-OPENSCREEN: National Infrastructure for Chemical Biology, Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, 14220 Prague 4, Czech Republic., Hrcka Krausova B; Laboratory of Cellular Neurophysiology, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague 4, Czech Republic., Adla SK; Dept. of Neurosteroids, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic., Slavikova B; Dept. of Neurosteroids, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic., Chodounska H; Dept. of Neurosteroids, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic., Kratochvil M; Dept. of Bioinformatics, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic., Vondrasek J; Dept. of Bioinformatics, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic., Sedlak D; CZ-OPENSCREEN: National Infrastructure for Chemical Biology, Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, 14220 Prague 4, Czech Republic., Balastik M; Laboratory of Molecular Neurobiology, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague 4, Czech Republic. Electronic address: martin.balastik@fgu.cas.cz., Kudova E; Dept. of Neurosteroids, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic. Electronic address: kudova@uochb.cas.cz.

Publikováno v: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2024 May; Vol. 239, pp. 106464. Date of Electronic Publication: 2024 Jan 19.

Pregnane-based steroids are novel positive NMDA receptor modulators that may compensate for the effect of loss-of-function disease-associated GRIN mutations.

Autor: Kysilov B; Institute of Physiology CAS, Prague 4, Czech Republic.; Third Faculty of Medicine, Charles University in Prague, Prague 10, Czech Republic., Hrcka Krausova B; Institute of Physiology CAS, Prague 4, Czech Republic., Vyklicky V; Institute of Physiology CAS, Prague 4, Czech Republic., Smejkalova T; Institute of Physiology CAS, Prague 4, Czech Republic., Korinek M; Institute of Physiology CAS, Prague 4, Czech Republic., Horak M; Institute of Physiology CAS, Prague 4, Czech Republic., Chodounska H; Institute of Organic Chemistry and Biochemistry CAS, Prague 6, Czech Republic., Kudova E; Institute of Organic Chemistry and Biochemistry CAS, Prague 6, Czech Republic., Cerny J; Institute of Physiology CAS, Prague 4, Czech Republic., Vyklicky L; Institute of Physiology CAS, Prague 4, Czech Republic.

Publikováno v: British journal of pharmacology [Br J Pharmacol] 2022 Aug; Vol. 179 (15), pp. 3970-3990. Date of Electronic Publication: 2022 Apr 08.

Effects of Pregnanolone Glutamate and Its Metabolites on GABA A and NMDA Receptors and Zebrafish Behavior.

Autor: Abramova V; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic.; Charles University, Third Faculty of Medicine, Ruska 87, 100 00 Prague 10,Czech Republic., Leal Alvarado V; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Hill M; Institute of Endocrinology, Narodni 8, 116 94 Prague 1, Czech Republic., Smejkalova T; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Maly M; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Vales K; Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic.; National Institute of Mental Health, Topolova 748, 250 67 Klecany, Czech Republic., Dittert I; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Bozikova P; Institute of Biotechnology CAS, Prumyslova 595, 252 50 Vestec, Czech Republic., Kysilov B; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Hrcka Krausova B; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Vyklicky V; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Balik A; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Fili K; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic.; Charles University, Third Faculty of Medicine, Ruska 87, 100 00 Prague 10,Czech Republic., Korinek M; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Chodounska H; Institute of Organic Chemistry and Biochemistry CAS, Flemingovo nam. 2, 166 10 Prague 2, Czech Republic., Kudova E; Institute of Organic Chemistry and Biochemistry CAS, Flemingovo nam. 2, 166 10 Prague 2, Czech Republic., Ciz D; IT4Innovations National Supercomputing Center, Studentska 6231/1B, 708 00 Ostrava-Poruba, Czech Republic., Martinovic J; IT4Innovations National Supercomputing Center, Studentska 6231/1B, 708 00 Ostrava-Poruba, Czech Republic., Cerny J; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Bartunek P; Institute of Molecular Genetics CAS, Videnska 1083, 142 20 Prague, Czech Republic.; CZ-OPENSCREEN, Institute of Molecular Genetics CAS, Videnska 1083, 142 20 Prague 4, Czech Republic., Vyklicky L; Laboratory of Cellular Neurophysiology, Institute of Physiology CAS, Videnska 1083, 142 20 Prague 4, Czech Republic.

Publikováno v: ACS chemical neuroscience [ACS Chem Neurosci] 2023 May 17; Vol. 14 (10), pp. 1870-1883. Date of Electronic Publication: 2023 May 01.

The Neuroactive Steroid Pregnanolone Glutamate: Anticonvulsant Effect, Metabolites and Its Effect on Neurosteroid Levels in Developing Rat Brains.

Autor: Kudova E; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 16000 Prague, Czech Republic., Mares P; Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic., Hill M; Institute of Endocrinology, Narodni 8, 11694 Prague, Czech Republic., Vondrakova K; Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.; Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic., Tsenov G; Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.; Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic.; Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Strada le Grazie 8, 37134 Verona, Italy., Chodounska H; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 16000 Prague, Czech Republic., Kubova H; Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic., Vales K; Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.; Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic.

Publikováno v: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2021 Dec 30; Vol. 15 (1). Date of Electronic Publication: 2021 Dec 30.

Pitfalls of NMDA Receptor Modulation by Neuroactive Steroids. The Effect of Positive and Negative Modulation of NMDA Receptors in an Animal Model of Schizophrenia.

Autor: Holubova K; National Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic., Chvojkova M; National Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic., Hrcka Krausova B; Institute of Physiology CAS, Videnska 1083, 14220 Prague, Czech Republic., Vyklicky V; Institute of Physiology CAS, Videnska 1083, 14220 Prague, Czech Republic., Kudova E; Institute of Organic Chemistry and Biochemistry CAS, Flemingovo namesti 542/2, 16000 Prague, Czech Republic., Chodounska H; Institute of Organic Chemistry and Biochemistry CAS, Flemingovo namesti 542/2, 16000 Prague, Czech Republic., Vyklicky L; Institute of Physiology CAS, Videnska 1083, 14220 Prague, Czech Republic., Vales K; National Institute of Mental Health, Topolova 748, 25067 Klecany, Czech Republic.; Institute of Physiology CAS, Videnska 1083, 14220 Prague, Czech Republic.

Publikováno v: Biomolecules [Biomolecules] 2021 Jul 14; Vol. 11 (7). Date of Electronic Publication: 2021 Jul 14.