Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Chittoor R Narahari"'
Autor:
Douglas D. Frey, Chittoor R. Narahari, Sanchayita Ghose, Ryan K. Swanson, Hui Guo, Ronald Bates, Sevda Deldari, Zheng Jian Li, Yang Liu
Publikováno v:
Journal of Chromatography A. 1568:108-122
Chromatofocusing is investigated as an alternative to protein A chromatography for the initial capture step in a purification process for several monoclonal antibodies and antibody fusion products. For comparison, this work also investigates the use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::426c286407494ead23f306f3aef6b6e0
https://doi.org/10.1016/j.chroma.2018.07.028
https://doi.org/10.1016/j.chroma.2018.07.028
Publikováno v:
AIChE Journal. 48:561-571
An efficient, general strategy was developed for numerically solving the system of equations that describe a pH gradient under local-equilibrium conditions when the elution buffer contains arbitrary numbers of both adsorbed and unadsorbed buffering s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b22e4fedb06459dc0db70e62eddfe5c8
https://doi.org/10.1002/aic.690480313
https://doi.org/10.1002/aic.690480313
Publikováno v:
Journal of Chromatography A. 825:115-126
The preparative-scale separation of two proteins into adjoined, pure bands was accomplished using a novel, hybrid chromatography method which employs chromatofocusing using a self-sharpening pH front and displacement development. The method eliminate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10a1f04e7f58fadde7ab26b1380d97d0
https://doi.org/10.1016/s0021-9673(98)00718-3
https://doi.org/10.1016/s0021-9673(98)00718-3
Autor:
Lisa Randers-Eichhorn, Douglas D. Frey, Govind Rao, Natraj Ramasubramanyan, John Strong, Chittoor R. Narahari
Publikováno v:
Biotechnology progress. 17(1)
Green fluorescent protein (GFP), which fluoresces in the green region of the visible spectrum and is widely used as a reporter for gene expression and regulation, was overexpressed in the JM105 strain of Escherichia coli transformed with pBAD-GFP. A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c917e1ff82ecd522e54096670170989
https://pubmed.ncbi.nlm.nih.gov/11170493
https://pubmed.ncbi.nlm.nih.gov/11170493