Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Chisato Sekikawa"'
Autor:
Sakayu Shimizu, Chisato Sekikawa, Tatsuo Tsunoda, Hideaki Togashi, Fujio Mizukami, Nobuyuki Horinouchi, Yo-hei Suzuki, Jun Ogawa, Miki Egami, Isao Masuda, Takayuki Y. Nara, Seigo Ono
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 68:181-186
Deoxyriboaldolase from Klebsiella pneumonia (KpDERA) was immobilized on interparticle pore type mesoporous silica (IMS), which consists of aggregates of hexagonally close-packed silica nanoparticles. Incubation of the free KpDERA in 300 mM acetaldehy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::16850aa56787b71662af12b510ec81cc
https://doi.org/10.1016/j.molcatb.2010.10.008
https://doi.org/10.1016/j.molcatb.2010.10.008
Autor:
Kanako Hisamatsu, Chisato Sekikawa, Fujio Mizukami, Hideaki Togashi, Tatsuo Tsunoda, Takayuki Y. Nara, Keita Inoh, Kengo Sakaguchi
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 64:107-112
Alanine racemase from Geobacillus stearothermophilus was physically immobilized on folded-sheet mesoporous silica (FSM) with pore diameters of 4 or 8.5 nm, and the activity, thermal stability, and reusability of the racemase were examined. The FSM wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2f19e5fa4123a5fb58a4984867bcb366
https://doi.org/10.1016/j.molcatb.2010.02.011
https://doi.org/10.1016/j.molcatb.2010.02.011
Autor:
Chisato Sekikawa, Fujio Mizukami, Hideaki Togashi, Takayuki Y. Nara, Kengo Sakaguchi, Tatsuo Tsunoda
Publikováno v:
Biotechnology Progress. 25:1071-1077
We established a 96-well-plate-based refolding screening system using zeolite. In this system, protein denatured and solubilized with 6 M guanidine hydrochloride is adsorbed onto zeolite placed in a 96-well plate. The refolding conditions can be test
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35a0f0b004cd05249a0091d32786c3a6
https://doi.org/10.1002/btpr.221
https://doi.org/10.1002/btpr.221
Autor:
Chisato Sekikawa, Fujio Mizukami, Masayuki Kawakami, Takayuki Y. Nara, Hideaki Togashi, Tatsuo Tsunoda, Nakatsugu Yaginuma, Kengo Sakaguchi
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 68:68-73
Zeolites are microporous crystalline aluminosilicates with a highly ordered structure. Using zeolite beta as an adsorbent, denatured/reduced hen egg lysozyme was refolded to the active form at high concentrations. The denatured/reduced lysozyme was a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::173f9f2023a7fd2caa81c3c0eea8baef
https://doi.org/10.1016/j.colsurfb.2008.09.012
https://doi.org/10.1016/j.colsurfb.2008.09.012
Autor:
Masayuki Kawakami, Fujio Mizukami, Tatsuo Tsunoda, Hideaki Togashi, Chisato Sekikawa, Nakatsugu Yaginuma, Takayuki Y. Nara, Kengo Sakaguchi
Publikováno v:
Biotechnology Progress. 25:200-206
We used zeolite beta as an adsorbing matrix to refold recombinant lactate dehydrogenase (LDH) protein collected as an insoluble aggregate from a bacterial expression system. The adsorption isotherm revealed that 1 g of zeolite adsorbed 200 mg of dena
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5f5350b7cb3f30af11479bfaa0f4d94
https://doi.org/10.1002/btpr.107
https://doi.org/10.1002/btpr.107
Autor:
Yukiya Hakuta, Chisato Sekikawa, Yo-hei Suzuki, Tsunoda Tatsuro, Naofumi Nagai, Fujio Mizukami, Takayuki Y. Nara
Publikováno v:
Journal of Materials Chemistry. 22:3234
The texture of porous films prepared from boehmite nanofibres changed from the lengthwise orientation to the random orientation with the addition of ammonia, and with this change, the shape of the pores in the films turned from slit-like into mesh-li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d342485607b39e232514f8e39c923e68
https://doi.org/10.1039/c2jm15704g
https://doi.org/10.1039/c2jm15704g