Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Chimeric nuclease"'
Publikováno v:
eLife, Vol 10 (2021)
eLife
eLife
Mobile genetic elements, elements that can move horizontally between genomes, have profound effects on their host's fitness. The phage-inducible chromosomal island-like element (PLE) is a mobile element that integrates into the chromosome of Vibrio c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e452ae8ffe2774e78f25866d17852c2
https://escholarship.org/uc/item/8qm3j8kz
https://escholarship.org/uc/item/8qm3j8kz
Mobile genetic elements, elements that can move horizontally between genomes, have profound effects on their hosts fitness. The PLE is a mobile element that integrates into the chromosome of Vibrio cholerae and parasitizes the bacteriophage ICP1 to m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68229309dc0d821cba68dbfddc42e1e1
https://doi.org/10.1101/2021.02.21.432181
https://doi.org/10.1101/2021.02.21.432181
Autor:
Linghui Hou, Jixi Li, Chengdong Zhang, Hongmao Liu, Hongyan Wang, Feng Lan, Jingjing Wei, Dong Liu, Daru Lu, Wang Shuai, Yongming Wang, Hu Ziying, Miaomiao Li
The compact CRISPR/Cas9 system, which can be delivered by adeno-associated virus (AAV), is a promising platform for therapeutic applications. However, current compact Cas9 nucleases have limited activity, targeting scope and specificity. Here, we ide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a422ca519e851924d855303f42a2e45c
https://doi.org/10.1101/2020.09.29.316661
https://doi.org/10.1101/2020.09.29.316661
Autor:
Sonya J. Franklin, Joel T. Welch
Publikováno v:
Comments on Inorganic Chemistry. 26:127-164
De novo design is a powerful tool to investigate the active site of enzymatic metalloproteins, in a smaller, defined model system. It is also a way to build or combine activity and selectivity in unique ways, not seen biologically. We are utilizing p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5462c29d5c6083875e400b24758b2a03
https://doi.org/10.1080/02603590500201188
https://doi.org/10.1080/02603590500201188
Publikováno v:
Methods in Molecular Biology ISBN: 9781627037600
Gene targeting by homologous recombination at chromosomal endogenous loci has traditionally been considered a low-efficiency process. However, the effectiveness of such so-called genome surgery or genome editing has recently been drastically improved
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f9f36691d891016d56c7669d2c2de5bc
https://doi.org/10.1007/978-1-62703-761-7_21
https://doi.org/10.1007/978-1-62703-761-7_21
Autor:
William T. Garrard, Chien Ping Liang
Publikováno v:
Methods. 17:95-103
In the past decade, site-specific chromosomal DNA cleavage mediated by DNA endonucleases has been used to examine diverse aspects of chromosome structure and function in eukaryotes, such as DNA topology, replication, transcription, recombination, and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c58f93a5902e4b4bba5765d0025921e
https://doi.org/10.1006/meth.1998.0721
https://doi.org/10.1006/meth.1998.0721
Publikováno v:
Insect biochemistry and molecular biology. 43(1)
Engineered nucleases are proteins that are able to cleave DNA at specified sites in the genome. These proteins have recently been used for gene targeting in a number of organisms. We showed earlier that zinc finger nucleases (ZFNs) can be used for ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81e508b41adad2cdf62a679dc6f05c0e
https://pubmed.ncbi.nlm.nih.gov/23142190
https://pubmed.ncbi.nlm.nih.gov/23142190
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 1, Iss C (2012)
Molecular therapy. Nucleic acids
Molecular therapy. Nucleic acids
Researchers have long recognized that the random introduction of new DNA into the genome could result in unpredictable genetic effects due to bilateral influences between transgenic and endogenous sequences. Hence, precise editing or replacement of m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d616de5b459c6b4701963ed41d8f543d
http://www.sciencedirect.com/science/article/pii/S2162253116300634
http://www.sciencedirect.com/science/article/pii/S2162253116300634