Výsledky vyhledávání - "Chikashi, Toyoshima"

Autor: Ryuta, Kanai, Flemming, Cornelius, Bente, Vilsen, Chikashi, Toyoshima

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 119(15)

Cryoelectron microscopy (cryo-EM) was applied to Na+,K+-ATPase (NKA) to determine the structures of two E2P states, one (E2PATP) formed by ATP and Mg2+ in the forward reaction, and the other (E2PPi) formed by inorganic phosphate (Pi) and Mg2+ in the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c29e6b52c42fd305a6cbff94407e357c
https://pubmed.ncbi.nlm.nih.gov/35380894

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Cryoelectron microscopy of Na+,K+-ATPase in the two E2P states with and without cardiotonic steroids

Autor: Ryuta Kanai, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima

Publikováno v: Kanai, R, Cornelius, F, Vilsen, B & Toyoshima, C 2022, ' Cryoelectron microscopy of Na +,K +-ATPase in the two E2P states with and without cardiotonic steroids ', PNAS (Proceedings of the National Academy of Sciences of the United States of America), vol. 119, no. 15, e2123226119 . https://doi.org/10.1073/pnas.2123226119

Significance The E2P state of Na + ,K + -ATPase, in which the ATPase is phosphorylated and of low affinity to Na + with the extracellular gate opened, shows different biochemical properties depending on whether the phosphate is transferred from ATP i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c1b6e220a20ae95495f7eae7806b003
https://pure.au.dk/portal/da/publications/cryoelectron-microscopy-of-nakatpase-in-the-two-e2p-states-with-and-without-cardiotonic-steroids(336736ba-7afe-41e3-b4ff-7afcc9b0b5dd).html

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What ATP binding does to the Ca 2+ pump and how nonproductive phosphoryl transfer is prevented in the absence of Ca 2+

Autor: Chikashi Toyoshima, Rie Nakajima, Haruo Ogawa, Yoshiki Kabashima

Publikováno v: Proceedings of the National Academy of Sciences. 117:18448-18458

Significance During phosphoryl transfer from ATP in P-type ion translocating ATPases, very large-scale and complex structural changes take place, resulting in occlusion of ions to be translocated. In the present report, we describe a new crystal stru

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7f6935c4c225d25bdd3c78b218acca97
https://doi.org/10.1073/pnas.2006027117

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Akademický článek

ATPase-dependent auto-phosphorylation of the open condensin hinge diminishes DNA binding

Autor: Yuko Akai, Ryuta Kanai, Norihiko Nakazawa, Masahiro Ebe, Chikashi Toyoshima, Mitsuhiro Yanagida

Publikováno v: Open Biology, Vol 4, Iss 12 (2014)

Condensin, which contains two structural maintenance of chromosome (SMC) subunits and three regulatory non-SMC subunits, is essential for many chromosomal functions, including mitotic chromosome condensation and segregation. The ATPase domain of the

Externí odkaz: https://doaj.org/article/251823daae0c424282b9f4fb6979f0d4

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An intramolecular scrambling path controlled by a gatekeeper in Xkr8 phospholipid scramblase

Autor: Takaharu Sakuragi, Shigekazu Nagata, Ryuta Kanai, E. Onishi, Akihisa Tsutsumi, Akiko Nakagawa, Chikashi Toyoshima, H. Narita, Masahide Kikkawa, Takeshi Baba, T. Miyazaki

Xkr8-Basigin is a phospholipid scramblase at plasma membranes that is activated by kinase or caspase. We investigated its structure at a resolution of 3.8Å. Its membrane-spanning region had a cuboid-like structure stabilized by salt bridges between

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8767d20af161aeb3925e97ca2c2324db
https://doi.org/10.1101/2021.05.06.442885

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Binding of cardiotonic steroids to Na+,K+-ATPase in the E2P state

Autor: Ryuta Kanai, Flemming Cornelius, Kanna Motoyama, Bente Vilsen, Haruo Ogawa, Chikashi Toyoshima

Publikováno v: Kanai, R, Cornelius, F, Ogawa, H, Motoyama, K, Vilsen, B & Toyoshima, C 2021, ' Binding of cardiotonic steroids to Na +,K +-ATPase in the E2P state ', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 1, 2020438118 . https://doi.org/10.1073/pnas.2020438118
Kanai, R, Cornelius, F, Ogawa, H, Motoyama, K, Vilsen, B & Toyoshima, C 2021, ' Binding of cardiotonic steroids to Na +,K +-ATPase in the E2P state ', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 1, e2020438118 . https://doi.org/10.1073/pnas.2020438118

The sodium pump (Na+, K+-ATPase, NKA) is vital for animal cells, as it actively maintains Na+ and K+ electrochemical gradients across the cell membrane. It is a target of cardiotonic steroids (CTSs) such as ouabain and digoxin. As CTSs are almost uni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::521d5eef2fd6c207f669b9901d57cd01
https://pure.au.dk/portal/da/publications/binding-of-cardiotonic-steroids-to-nakatpase-in-the-e2p-state(2e957c44-03f9-4c8f-8283-f06307775924).html

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Binding of cardiotonic steroids to Na

Autor: Ryuta, Kanai, Flemming, Cornelius, Haruo, Ogawa, Kanna, Motoyama, Bente, Vilsen, Chikashi, Toyoshima

Publikováno v: Proc Natl Acad Sci U S A

The sodium pump (Na(+), K(+)-ATPase, NKA) is vital for animal cells, as it actively maintains Na(+) and K(+) electrochemical gradients across the cell membrane. It is a target of cardiotonic steroids (CTSs) such as ouabain and digoxin. As CTSs are al

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6842cd69c8d2a81c0285e21c002d88ae
https://pubmed.ncbi.nlm.nih.gov/33318128

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