Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Chihiro Uematsu"'
Autor:
Junko Tanaka, Tatsuo Nakagawa, Kunio Harada, Chigusa Morizane, Hidenori Tanaka, Satoshi Shiba, Akihiro Ohba, Susumu Hijioka, Erina Takai, Shinichi Yachida, Yoshio Kamura, Takeshi Ishida, Takahide Yokoi, Chihiro Uematsu
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract A highly sensitive and highly multiplexed quantification technique for nucleic acids is necessary to predict and evaluate cancer treatment by liquid biopsy. Digital PCR (dPCR) is a highly sensitive quantification technique, but conventional
Externí odkaz:
https://doaj.org/article/64bb1301ddb449c4b50b7765f409a5e0
Autor:
Atsushi Takeda, Masatoshi Nakamura, Hiroaki Fujii, Chihiro Uematsu, Tatsuya Minamino, Paul A Adlard, Ashley I Bush, Haruna Tamano
Publikováno v:
PLoS ONE, Vol 9, Iss 12, p e115923 (2014)
We examined an idea that short-term cognition is transiently affected by a state of confusion in Zn2+ transport system due to a local increase in amyloid-β (Aβ) concentration. A single injection of Aβ (25 pmol) into the dentate gyrus affected dent
Externí odkaz:
https://doaj.org/article/7c95227c19064aaa9604ea25e7ef157d
Autor:
Narihito Ogawa, Shoya Toyoshima, Shinya Sekikawa, Masahiro Ishijima, Kyosuke Katagiri, Chihiro Uematsu, Tatsuya Hirano, Akihito Ootaka, Jun Suzuki
Publikováno v:
Journal of Pesticide Science. 48:11-16
Publikováno v:
Synlett. 32:2071-2074
This paper describes a stereoselective synthesis of (–)-heliannuol E through intramolecular cyclization of a phenol mesylate. The introduction of the aromatic group was achieved by α-selective propargyl substitution of a propargylic phosphate.
Autor:
Kazushige Sugie, Hideki Niimi, Tomohiro Ueno, Hideyuki Noda, Yuichi Uchiho, Shunsuke Kawabe, Masako Ishimaru, Hiroko Tada, Chihiro Uematsu, Fumiaki Hamazato, Isao Kitajima
We developed a novel pretreatment procedure that destroys the plasma membrane of platelets but not the bacterial cell wall, allowing viable bacterial ATP in septic blood samples to be measured with minimal human-derived ATP background. After the pret
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e8f8ab4c7ed03861e12d8a823a06f807
https://doi.org/10.21203/rs.3.rs-2081889/v1
https://doi.org/10.21203/rs.3.rs-2081889/v1
Publikováno v:
2021 IEEE 34th International Conference on Micro Electro Mechanical Systems (MEMS).
In this paper, we propose a combination of digital polymerase chain reaction (dPCR) and melting curve analysis for the highly multiplexed genotyping of cell-free DNA (cfDNA) with high sensitivity. A silicon chip with 7.7x104 high-aspect-ratio wells w
Autor:
Tatsuo, Nakagawa, Junko, Tanaka, Kunio, Harada, Akiko, Shiratori, Yuzuru, Shimazaki, Takahide, Yokoi, Chihiro, Uematsu, Yoshinobu, Kohara
Publikováno v:
Analytical chemistry. 92(17)
Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient P
Autor:
Yoshio Kamura, Kunio Harada, Junko Tanaka, Chihiro Uematsu, Akiko Shiratori, Takeshi Ishida, Tatsuo Nakagawa
Publikováno v:
Cancer Research. 81:579-579
Background Digital PCR (dPCR) is a promising method for liquid biopsies and is able to quantify nucleic acids more sensitively than real-time PCR. However, dPCR has a large fluctuation in the fluorescence intensity of the droplets or wells due to ins
Autor:
Tatsuo Nakagawa, Kunio Harada, Junko Tanaka, Akiko Shiratori, Masao Kamahori, Takahide Yokoi, Yoshinobu Kohara, Chihiro Uematsu, Yuzuru Shimazaki
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Scientific Reports
Scientific Reports
Digital PCR (dPCR) has been developed as a method that can quantify nucleic acids more sensitively than real-time PCR. However, dPCR exhibits large fluctuations in the fluorescence intensity of the compartment, resulting in low accuracy. The main cau
Autor:
Akiko Shiratori, Yuzuru Shimazaki, Tatsuo Nakagawa, Junko Tanaka, Kunio Harada, Chihiro Uematsu
Publikováno v:
Japanese Journal of Applied Physics. 60:037002
A dense array of surfactant-stabilized water droplets (20 μm in diameter) floating on the surface of fluorinated oil was prepared. More than 30000 droplets were planarly arrayed in an 8 mm squared area using the “droplet guide”, an assembly of t