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pro vyhledávání: '"Chihiro Fukuhara"'
Autor:
Yoshifumi Adachi, Chihiro Fukuhara
Publikováno v:
Analytical biochemistry. 431(1)
A simple, rapid, and efficient site-directed mutagenesis method using TA strategy with synthetic mutagenic oligonucleotides is described. Briefly, a 3' A-overhang vector was prepared by polymerase chain reaction (PCR) using a classical Taq polymerase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67e0a9c81ac7081be8da1539894faa50
https://pubmed.ncbi.nlm.nih.gov/22960560
https://pubmed.ncbi.nlm.nih.gov/22960560
